A5076973759- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- Hippo pathway signaling and YAP/TAZ
- Lipid Membrane Structure and Behavior
- Wnt/β-catenin signaling in development and cancer
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Liver physiology and pathology
- Cancer Cells and Metastasis
- Renal and related cancers
- Caveolin-1 and cellular processes
- Bacterial biofilms and quorum sensing
- Genetic and Kidney Cyst Diseases
- Barrier Structure and Function Studies
- Signaling Pathways in Disease
- Blood properties and coagulation
- Neonatal Respiratory Health Research
- Vibrio bacteria research studies
- Cancer-related gene regulation
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
University of California, San Francisco
2013-2022
University of San Francisco
1995-2015
Institute of Molecular Biology and Biophysics
1997-2012
University of California, Berkeley
1997-2011
Beijing Normal University
2006
Kansas State University
2006
Northeast Normal University
2006
National Museum of Natural Science
2006
Nanjing Medical University
2006
University of Iowa
2006
Four transgenic Nicotiana tabacum plants were generated that expressed a murine monoclonal antibody kappa chain, hybrid immunoglobulin A-G heavy joining and rabbit secretory component, respectively. Successive sexual crosses between these filial recombinants resulted in all four protein chains simultaneously. These assembled into functional, high molecular weight recognized the native streptococcal antigen I/II cell surface adhesion molecule. In plants, single cells are able to assemble...
Classically, the polymeric immunoglobulin receptor and its ligand, IgA, are thought to be sorted from basolateral early endosomes into transcytotic vesicles that directly fuse with apical plasma membrane. In contrast, we have found in MDCK cells IgA is delivered only then cell surface. When internalized surface of accumulate under membrane a compartment accessible two apically added markers: anti-secretory component Fab fragments, avidin biotinylated pole cell. This accumulation occurs...
Epithelial cells polarize and orient polarity in response to cell-cell cell-matrix adhesion. Although there has been much recent progress understanding the general polarizing machinery of epithelia, it is largely unclear how this controlled by extracellular environment. To explore signals from interactions that control orientation cell polarity, we have used three-dimensional culture systems which Madin-Darby canine kidney (MDCK) form polarized, lumen-containing structures. We show...
We have analyzed conserved domains in t-SNAREs [soluble N -ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptors the target membrane], proteins that are believed to be involved fusion of transport vesicles with their membrane. By using a sensitive computer method, generalized profile we were able identify new homology domain is common two families previously identified act as t-SNAREs, syntaxin and SNAP-25 (synaptosome-associated 25 kDa) families, which therefore...
Pseudomonas aeruginosa, an opportunistic pathogen, is capable of establishing both chronic and acute infections in compromised hosts. Previous studies indicated that P. aeruginosa displays either a cytotoxic or invasive phenotype corneal epithelial cells. In this study, we used polarized MDCK cells for vitro infection confirmed isolates can be broadly differentiated into two groups, expressing phenotype. vivo were performed to determine if strains displayed differential pathology. Invasion...
The endosomal compartment of polarized epithelial cells is a major crossroads for membrane traffic. Proteins entering this from the cell surface are sorted transport to one several destinations: recycling original surface, targeting lysosomes degradation, or transcytosis opposite surface. polymeric immunoglobulin receptor (pIgR), which normally transcytosed basolateral apical was used as model dissect signals that mediate sorting event. When exogenous expressed in Madin-Darby Canine Kidney...