A5001221114- Developmental Biology and Gene Regulation
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Cellular Mechanics and Interactions
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurobiology and Insect Physiology Research
- Cancer therapeutics and mechanisms
- Genomics and Chromatin Dynamics
- Invertebrate Immune Response Mechanisms
- Plant Molecular Biology Research
- Animal Genetics and Reproduction
- Axon Guidance and Neuronal Signaling
- Genetics, Aging, and Longevity in Model Organisms
- Silk-based biomaterials and applications
- DNA Repair Mechanisms
- Marine Biology and Environmental Chemistry
- DNA and Nucleic Acid Chemistry
- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Plant Disease Resistance and Genetics
- Marine Ecology and Invasive Species
- Insect symbiosis and bacterial influences
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
University of Pennsylvania
2015-2025
California Institute for Regenerative Medicine
2008-2025
University of California, Los Angeles
2005
University of Cambridge
2005
Rockefeller University
1990-2001
California University of Pennsylvania
2001
European Molecular Biology Organization
1999
Stanford University
1993
University of Colorado Boulder
1988
University of California, San Francisco
1984-1988
A temperature-sensitive DNA topoisomerase II mutant of the yeast Saccharomyces cerevisiae has been identified. Genetic analysis shows that a single recessive nuclear mutation is responsible for both growth and enzymatic activity. Thus, essential viability most probably in structural gene. Experiments with synchronized cells show at nonpermissive temperature can undergo one, only round replication. These are arrested medial division. Analysis 2-microns plasmid from these it to be form...
For more than 100 years, the fruit fly
Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here, we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study. With over 44,000 nuclei 6000 cells analyzed, identification rare types,...
Abstract We describe 83 recessive autosomal male-sterile mutations, generated by single P element mutagenesis in Drosophila melanogaster. Each mutation has been localized to a lettered subdivision of the polytene map. Reversion analyses, as well complementation tests using available chromosomal deficiencies, indicate that insertions are responsible for mutant phenotypes. These mutations represent 63 groups, 58 which required spermatogenesis. Phenotypes spermatogenesis mutants were analyzed...
Mutations in top, the structural gene for Escherichia coli DNA topoisomerase I, have been identified and mapped at 28 min on chromosome, near cysB. Strains carrying deletions of top are viable. The mutations, however, do exert pleiotropic effects transcription transposition. Mutants lacking I a more rapid rate induction higher level catabolite-sensitive enzymes including tryptophanase beta-galactosidase. This general activation by mutations can be attributed to an increase negative...
We are examining the development of segmental body pattern Drosophila by immunolocalization engrailed, a developmental regulatory protein that maintains subdivisions in embryo, and is expressed spatially restricted (striped) manner persists while being established refined. A network among pair-rule segmentation genes establishes striped engrailed expression. In general, mutations particular affect either even- or odd-numbered stripes. For example, fushi tarazu odd-paired delete even-numbered...
Spermatogenesis relies on the function of germ-line stem cells, as a continuous supply differentiated spermatids is produced throughout life. In Drosophila, there must also be somatic cells that produce cyst accompany germ spermatogenesis. By lineage tracing, we demonstrate existence such and confirm cells. The likely correspond to ultrastructurally described progenitor for both are anchored around hub non-dividing located at testis tip. We then address whether regulate behavior progenitors...
Stem cells divide asymmetrically, regenerating a parental stem cell and giving rise to daughter with distinct fate. In many lineages, this undergoes several amplificatory mitoses, thus generating more that embark on the differentiation program specific for given lineage. Spermatogenesis in Drosophila is model system identify molecules regulating lineages. Mutations at two previously identified loci, bag-of-marbles (bam) benign gonial neoplasm (bgcn), prevent progression through...
Abstract The ability to obtain single cell transcriptomes for stable types and dynamic states is ushering in a new era biology. We created the Tabula Drosophilae , atlas of adult fruit fly which includes 580k cells from 15 individually dissected sexed tissues as well entire head body. Over 100 researchers community contributed annotations >250 distinct across all tissues. provide an in-depth analysis type-related gene signatures transcription factor markers, sexual dimorphism, whole...
The pair-rule gene, odd-paired (opa), is essential for parasegmental subdivision of the Drosophila embryo. In addition to its previously defined role in activation wingless (wg) odd parasegments, we find that opa required timely wg remaining parasegments and engrailed (en) all parasegments. encodes a zinc finger protein with fingers homologous those segment polarity gene ciD, human glioblastoma GLI Caenorhabditis elegans sex determination tra-1. Previous work showed activity was...
Summary Drosophila melanogaster has emerged as an important model system for the study of both stem cell biology and aging. Much is known about how molecular signals from somatic niche regulate adult cells in germline, a variety environmental factors well single point mutations have been shown to affect lifespan. Relatively little known, however, aging affects specific populations cells, particularly that may be susceptible aging‐related damage. Here we show male germline (GSCs) are lost...
Mutants of the yeast, Saccharomyces cerevisiae, deficient in DNA topoisomerase I activity have been identified. One mutant has normal when assayed at 25 degrees C and about 20% 36 C. Strains with this mutation grow normally all temperatures tested. The mapped to MAK1, a gene required for maintenance killer RNA. Three previously isolated mak1 mutants exhibit less than 1% our assay, but yet they normally. implications these results role cell are discussed.