A5006330388- RNA Research and Splicing
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Silk-based biomaterials and applications
- Single-cell and spatial transcriptomics
- Neurobiology and Insect Physiology Research
- Genomics and Chromatin Dynamics
- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Hippo pathway signaling and YAP/TAZ
- Environmental DNA in Biodiversity Studies
- Molecular Biology Techniques and Applications
- Insect Resistance and Genetics
- Protist diversity and phylogeny
- DNA Repair Mechanisms
- Cancer Cells and Metastasis
- Ubiquitin and proteasome pathways
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
Whitehead Institute for Biomedical Research
2021-2025
Howard Hughes Medical Institute
2021-2024
Massachusetts Institute of Technology
2022-2023
University of Michigan–Ann Arbor
2014-2020
Michigan United
2019
Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here, we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study. With over 44,000 nuclei 6000 cells analyzed, identification rare types,...
Intron gigantism, where genes contain megabase-sized introns, is observed across species, yet little known about its purpose or regulation. Here we identify a unique gene expression program utilized for the proper of with intron gigantism. We find that two Drosophila kl-3 and kl-5, are transcribed in spatiotemporal manner over course spermatocyte differentiation, which spans ~90 hours. The introns these megabases simple satellite DNA repeats comprise 99% loci, satellite-DNA containing...
ABSTRACT Drosophila sperm development is characterized by extensive post-transcriptional regulation whereby thousands of transcripts are preserved for translation during later stages. A key step in initiation the binding eukaryotic factor 4E (eIF4E) to 5′ mRNA cap. In addition canonical eIF4E-1, has multiple eIF4E paralogs, including four (eIF4E-3, -4, -5, and -7) that highly expressed testis. Among these, only eIF4E-3 been genetically. Here, using CRISPR/Cas9 mutagenesis, we determined...
SUMMARY In many organisms including Drosophila and mammals, male germ cells, particularly spermatocytes, have one of the most complex transcriptomes, where a large fraction genome noncoding DNA are transcribed. However, significance this widespread transcription remains enigmatic. Through study satellite in spermatocytes as representational model for transcription, we propose that is required to remodel chromatin preparation sperm packaging. We identified HP2, known heterochromatin protein,...
Cytoplasmic cilia, a specialized type of cilia in which the axoneme resides within cytoplasm rather than ciliary compartment, are proposed to allow for efficient assembly very long cilia. Despite being found diversely male gametes (e.g., Plasmodium falciparum microgametocytes and human Drosophila melanogaster sperm), little is known about cytoplasmic assembly. Here, we show that novel RNP granule containing mRNAs axonemal dynein motor proteins becomes highly polarized distal end during...
Abstract DNA damage by ultraviolet (UV) light poses a risk for mutagenesis and potential hindrance cell cycle progression. Cells cope with UV-induced through two general strategies to repair the damaged nucleotides promote progression in presence of UV-damaged DNA. Defining genetic pathways understanding how they function together enable effective tolerance UV remains an important area research. The structural maintenance chromosomes (SMC) proteins form distinct complexes that maintain...
Abstract In animals, PIWI-interacting RNAs (piRNAs) direct PIWI proteins to silence complementary targets such as transposons. Drosophila and other species with a maternally specified germline, piRNAs deposited in the egg initiate piRNA biogenesis progeny. However, Y chromosome loci cannot participate chain of intergenerational inheritance. How then can Y-linked be initiated? Here, using Suppressor Stellate ( Su(Ste) ), melanogaster locus model, we show that are made early male germline via...
Although introns are typically tens to thousands of nucleotides, there notable exceptions. In flies as well humans, a small number genes contain that more than 1000 times larger typical introns, exceeding hundreds kilobases (kb) megabases (Mb). It remains unknown why gigantic exist and how cells overcome the challenges associated with their transcription RNA processing. The Drosophila Y chromosome contains some largest identified date: multiple exceed 4Mb, accounting for over 99% gene span....
Abstract Cytoplasmic cilia, a specialized type of cilia in which the axoneme resides within cytoplasm rather than ciliary compartment, are proposed to allow efficient assembly very long cilia. Despite being found diversely male gametes (e.g. Plasmodium microgametocytes and human Drosophila sperm), little is known about cytoplasmic assembly. Here we show that novel RNP granule containing mRNAs for axonemal dynein motor proteins becomes highly polarized distal end during ciliogenesis sperm....
Abstract Although introns are typically tens to thousands of nucleotides, there notable exceptions. In flies as well humans, a small number genes contain that more than 1000 times larger typical introns, exceeding hundreds kilobases (kb) megabases (Mb). It remains unknown why gigantic exist and how cells overcome the challenges associated with their transcription RNA processing. The Drosophila Y chromosome contains some largest identified date: multiple exceed 4Mb, accounting for over 99%...
Abstract Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study (Li et al ., 2022). With over 44,000 nuclei 6,000 cells analyzed,...
Intron gigantism, where genes contain megabase-sized introns, is observed across species, yet little known about its purpose or regulation. Here we identify a unique gene expression program utilized for the proper of with intron gigantism. We find that two Drosophila kl-3 and kl-5, are transcribed in spatiotemporal manner over course spermatocyte differentiation, which spans ~90 hours. The introns these megabases simple satellite DNA repeats comprise 99% loci, satellite-DNA containing...
Abstract Drosophila sperm development is characterized by extensive post-transcriptional regulation whereby thousands of transcripts are preserved for translation during later stages. A key step in initiation the binding eukaryotic factor 4E (eIF4E) to 5’ mRNA cap. has multiple paralogs eIF4E, including four (eIF4E-3, -4, -5, and -7) that highly expressed testis. Other than eIF4E-3, none these been genetically. Here, using CRISPR/Cas9 mutagenesis, we determined eIF4E-5 essential male...