A5012225555- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Hospital for Sick Children
2023
Canada Research Chairs
2023
University of Toronto
2023
ABSTRACT Drosophila sperm development is characterized by extensive post-transcriptional regulation whereby thousands of transcripts are preserved for translation during later stages. A key step in initiation the binding eukaryotic factor 4E (eIF4E) to 5′ mRNA cap. In addition canonical eIF4E-1, has multiple eIF4E paralogs, including four (eIF4E-3, -4, -5, and -7) that highly expressed testis. Among these, only eIF4E-3 been genetically. Here, using CRISPR/Cas9 mutagenesis, we determined...