A5071036191- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Milk Quality and Mastitis in Dairy Cows
- Mycobacterium research and diagnosis
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- Glioma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Pluripotent Stem Cells Research
- Antimicrobial Resistance in Staphylococcus
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- SARS-CoV-2 detection and testing
- Corneal surgery and disorders
- Monoclonal and Polyclonal Antibodies Research
- Biosimilars and Bioanalytical Methods
- Neuroinflammation and Neurodegeneration Mechanisms
- T-cell and B-cell Immunology
Nanjing Medical University
2018-2024
University of Toronto
2011-2024
Asan Medical Center
2024
Dali University
2024
Second People's Hospital of Yunnan Province
2024
Affiliated Hospital of Southwest Medical University
2024
Dalian Medical University
2024
First Affiliated Hospital of Dalian Medical University
2024
McGill University
2009-2023
Occupational Cancer Research Centre
2022
In mammalian small intestine, a H+-coupled peptide transporter is responsible for the absorption of peptides arising from digestion dietary proteins. Recently cDNA clone encoding H+/peptide cotransporter has been isolated rabbit intestinal library (Fei, Y. J., Kanai, Y., Nussberger, S., Ganapathy, V., Leibach, F. H., Romero, M. F., Singh, S. K., Boron, W. and Hediger, A.(1994) Nature 368, 563-566). Screening human with probe derived resulted in identification which when expressed HeLa cells...
Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise regenerative medicine. For understanding pluripotency lineage differentiation mechanisms, a critical first step involves systematically cataloging essential genes (EGs) that are indispensable hPSC fitness, defined as cell reproduction in this study. To map genetic determinants of we performed genome-scale loss-of-function screens inducible Cas9 H1 line cultured on feeder laminin to identify...
Glioblastoma (GBM) patients suffer from a dismal prognosis, with standard of care therapy inevitably leading to therapy-resistant recurrent tumors. The presence cancer stem cells (CSCs) drives the extensive heterogeneity seen in GBM, prompting need for novel therapies specifically targeting this subset tumor-driving cells. Here, we identify CD70 as potential therapeutic target GBM CSCs.
Vasa (Vas) is a DEAD-box RNA-binding protein required in Drosophila at several steps of oogenesis and for primordial germ cell (PGC) specification. Vas associates with eukaryotic initiation factor 5B (eIF5B), this interaction has been implicated translational activation gurken mRNA the oocyte. expressed all ovarian germline cells, aspects vas -null phenotype suggest function regulating balance between stem cells (GSCs) their fate-restricted descendants. We used biochemical approach to...
Embryonic stem cells are maintained in a self-renewing and pluripotent state by multiple regulatory pathways. Pluripotent-specific transcriptional networks sequentially reactivated as somatic reprogram to achieve pluripotency. How epigenetic regulators modulate this process contribute cell reprogramming is not clear. Here we performed functional RNAi screen identify the earliest required for reprogramming. We identified components of SAGA histone acetyltransferase complex, particular Gcn5,...
Abstract Population scale sweeps of viral pathogens, such as SARS-CoV-2, require high intensity testing for effective management. Here, we describe “Systematic Parallel Analysis RNA coupled to Sequencing Covid-19 screening” (C19-SPAR-Seq), a multiplexed, scalable, readily automated platform SARS-CoV-2 detection that is capable analyzing tens thousands patient samples in single run. To address strict requirements control assay parameters and output demanded by clinical diagnostics, employ...
Abstract Loss of heterozygosity (LOH) was examined at 86 loci distributed on every chromosomal arm in 50 human ovarian tumors. Frequent allele losses were observed chromosomes 13q (42%), 17p 17q (45%), and Xp (41%). Deletion mapping chromosome 17 revealed a candidate gene the long distal to D17S41/S74 for cancer which is distant from locus early onset breast cancer. LOH found be concordant with 3p, 13q, suggesting that it may an event neoplastic development. These findings indicate multiple...
The first committed step in triterpenoid biosynthesis is the cyclization of oxidosqualene to polycyclic alcohols or ketones C30H50O. It catalyzed by single cyclase (OSC) enzymes that can carry out varying numbers carbocation rearrangements and, thus, generate triterpenoids with diverse carbon skeletons. OSCs from plant species have been cloned and characterized, large majority them catalyzing relatively few rearrangement steps. was recently predicted special must exist form friedelin,...
Phenotypic differences in drug responses have been associated with known pharmacogenomic loci, but many remain to be characterized. Therefore, we developed next‐generation sequencing (NGS) panels enable broad and unbiased inspection of genes that are involved pharmacokinetics (PKs) pharmacodynamics (PDs). These feature repetitively optimized probes capture up 114 PK/PD‐related high coverage (99.6%) accuracy (99.9%). Sequencing a Korean cohort ( n = 376) the enabled profiling actionable...
In recent years, circular RNAs (circRNAs) have been shown to critical regulatory roles in the resistance anti-cancer drugs. However, contributions of circRNAs sorafenib hepatocellular carcinoma (HCC) remain largely unknown. The present study aims explore involvement circFN1 and how is associated with miR-1205/E2F1 pathway, which demonstrated mediate this HCC cells. We investigated expression five paired sorafenib-sensitive HepG2 cells sorafenib-resistant (SR)-HepG2 by microarray analysis....
ABSTRACT Drosophila sperm development is characterized by extensive post-transcriptional regulation whereby thousands of transcripts are preserved for translation during later stages. A key step in initiation the binding eukaryotic factor 4E (eIF4E) to 5′ mRNA cap. In addition canonical eIF4E-1, has multiple eIF4E paralogs, including four (eIF4E-3, -4, -5, and -7) that highly expressed testis. Among these, only eIF4E-3 been genetically. Here, using CRISPR/Cas9 mutagenesis, we determined...
Summary Approximately 45 Holstein cows that were Mycobacterium paratuberculosis -positive on the basis of fecal culture results maintained at any one time in a 210-cow dairy herd. Farm management participated New York State Paratuberculosis Eradication Program. Paratuberculosis-positive grouped separately from paratuberculosis-negative cows, but they otherwise managed identically. During 1-year study, 180 paratuberculosisnegative and 113 clinically normal paratuberculosis-positive...
Octamer-binding transcription factor 4 (OCT4) was closely related to pancreatic cancer progression, but its regulation in by microRNA (miRNA) is not fully clear. OCT4-positive and OCT4-negative cells were isolated flow cytometry, it found that are enriched transplanted compared with the primary ones showed increasing proliferation sphere formation. The data of miRNA array assay miR-335 lower than negative ones. results confirmed tissue cell lines. Through expression analysis, underexpressed...
This study was carried out to evaluate the prevalence and clinical characterizations of gastric Helicobacter spp. infection dogs cats in Korea. The determined by urease test 78.4% 64%, respectively, although genus-specific PCR assay showed that it 82.3% 84%. Urease mapping results based on total positive rate tested tissues from clinically abnormal significantly higher than normal (p=0.0018; Odds ratio = 6.118; 95% Confidence Interval 1.96-19.103). These findings were consistent with which...
A one step reverse transcription PCR (RT-PCR) combined nested was set up to increase efficiency in the diagnosis of canine distemper virus (CDV) infection after developement PCR. Two primer sets were designed based on sequence nucleocapsid gene CDV Onderstepoort strain. One-step RT-PCR with outer pair revealed detect 10(2) PFU/ml. The sensitivity increased hundredfold using one-step Specificity also confirmed other related and peripheral blood mononuclear cells (PBMC) body secretes healthy...
Twelve strains of the methicillin-resistant Staphylococcus aureus (MRSA) recovered from hospitalized dogs were analyzed for in vitro antimicrobial susceptibility and virulence, genetically characterized by pulsed-field gel electrophoresis (PFGE). Antibiotic test showed that nearly all isolates resistant to β-lactam antibiotics tested fully susceptible glycopeptides. There no inhibitory activities among aminoglycosides. The 50% lethal dose (LD50) was determined intraperitoneal injection cell...