A5078842111- Amino Acid Enzymes and Metabolism
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Ion Channels and Receptors
- Trace Elements in Health
- Neuroscience and Neuropharmacology Research
- Drug Transport and Resistance Mechanisms
- Iron Metabolism and Disorders
- Pancreatic function and diabetes
- Metabolism and Genetic Disorders
- Vitamin C and Antioxidants Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Pregnancy and preeclampsia studies
- Magnesium in Health and Disease
- Vitamin D Research Studies
- Molecular Sensors and Ion Detection
- Hemoglobinopathies and Related Disorders
- Heavy Metal Exposure and Toxicity
- Electrolyte and hormonal disorders
- Vitamin K Research Studies
- Birth, Development, and Health
- Neurobiology and Insect Physiology Research
- Bioactive Compounds and Antitumor Agents
- Diet and metabolism studies
University of Bern
2015-2024
University Hospital of Bern
2015-2024
University Children’s Hospital Bern
2020
Brigham and Women's Hospital
1998-2007
Harvard University
1998-2007
University of Cologne
2006
ETH Zurich
1978-2006
Harvard University Press
1993-2003
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2001-2002
National Institutes of Health
2002
Three glutamate transporters have been identified in rat, including astroglial GLAST and GLT-1 a neuronal transporter EAAC1. Here we demonstrate that inhibition of the synthesis each subtype using chronic antisense oligonucleotide administration, vitro vivo, selectively specifically reduced protein expression function transporters. The loss glial or produced elevated extracellular levels, neurodegeneration characteristic excitotoxicity, progressive paralysis. EAAC1 did not elevate striatum...
The ability of intestinal mucosa to absorb dietary ferric iron is attributed the presence a brush-border membrane reductase activity that displays adaptive responses status. We have isolated complementary DNA, Dcytb (for duodenal cytochrome b), which encoded putative plasma di-heme protein in mouse mucosa. shared between 45 and 50% similarity b561 family reductases, was highly expressed enterocytes, induced when Xenopus oocytes cultured cells. Duodenal expression levels messenger RNA were...
Calcium is a major component of the mineral phase bone and serves as key intracellular second messenger. Postnatally, all bodily calcium must be absorbed from diet through intestine. Here we report properties transport protein (CaT1) cloned rat duodenum using an expression cloning strategy in Xenopus laevis oocytes, which likely plays role intestinal uptake calcium. CaT1 shows homology (75% amino acid sequence identity) to apical channel ECaC recently vitamin D-responsive cells rabbit kidney...
The major reabsorptive mechanism for D-glucose in the kidney is known to involve a low affinity high capacity Na+/glucose cotransporter, which located early proximal convoluted tubule segment S1, and has Na+ glucose coupling ratio of 1:1. Here we provide first molecular evidence this renal mechanism. We report characterization previously cloned human cDNA that codes protein with 59% identity cotransporter (SGLT1). Using expression studies Xenopus laevis oocytes demonstrate (termed SGLT2)...
Electrically silent Na(+)-(K+)-Cl- transporter systems are present in a wide variety of cells and serve diverse physiological functions. In chloride secretory absorbing epithelia, these cotransporters provide the entry mechanism crucial for transcellular transport. We have isolated cDNAs encoding two major electroneutral sodium-chloride transporters mammalian kidney, bumetanide-sensitive Na(+)-K(+)-Cl- symporter thiazide-sensitive Na(+)-Cl- cotransporter, characterized their functional...
In mammalian small intestine, a H+-coupled peptide transporter is responsible for the absorption of peptides arising from digestion dietary proteins. Recently cDNA clone encoding H+/peptide cotransporter has been isolated rabbit intestinal library (Fei, Y. J., Kanai, Y., Nussberger, S., Ganapathy, V., Leibach, F. H., Romero, M. F., Singh, S. K., Boron, W. and Hediger, A.(1994) Nature 368, 563-566). Screening human with probe derived resulted in identification which when expressed HeLa cells...
In all living cells, coordination of solute and water movement across cell membranes is critical importance for osmotic balance. The current concept that these processes are distinct biophysical nature. Here we report the expression cloning a liver cDNA encoding unique promiscuous channel (AQP9) confers high permeability both solutes water. AQP9 mediates passage wide variety non-charged including carbamides, polyols, purines, pyrimidines in phloretin- mercury-sensitive manner, whereas amino...
Electroneutral Na+:Cl- cotransport systems are involved in a number of important physiological processes including salt absorption and secretion by epithelia cell volume regulation. One group cotransporters is specifically inhibited the benzothiadiazine (thiazide) class diuretic agents can be distinguished from Na+:K+:2Cl- based on lack K+ requirement insensitivity to sulfamoylbenzoic acid diruetics like bumetanide. We report here isolation cDNA encoding thiazide-sensitive, electroneutral...