A5030989787- Ion Channels and Receptors
- Ion channel regulation and function
- Ion Transport and Channel Regulation
- Magnesium in Health and Disease
- Trace Elements in Health
- Amino Acid Enzymes and Metabolism
- Electrolyte and hormonal disorders
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Metal Extraction and Bioleaching
- Genetic and Kidney Cyst Diseases
- Protein Kinase Regulation and GTPase Signaling
- Connexins and lens biology
- Genetics and Neurodevelopmental Disorders
- Orbital Angular Momentum in Optics
- Potassium and Related Disorders
- Biomedical Research and Pathophysiology
- Parathyroid Disorders and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Cardiac electrophysiology and arrhythmias
- Kidney Stones and Urolithiasis Treatments
- Extraction and Separation Processes
- Genetic Syndromes and Imprinting
- Receptor Mechanisms and Signaling
First Affiliated Hospital of Kunming Medical University
2024
Kunming Medical University
2024
University of Alabama at Birmingham
2013-2023
Pediatric Nephrology of Alabama
2013-2023
Guangdong Provincial Occupational Disease Prevention Hospital
2023
Anhui Normal University
2015
Chinese Academy of Sciences
1999-2012
St. Vincent's Birmingham
2012
University of Alabama
2010
University of Birmingham
2007
Calcium is a major component of the mineral phase bone and serves as key intracellular second messenger. Postnatally, all bodily calcium must be absorbed from diet through intestine. Here we report properties transport protein (CaT1) cloned rat duodenum using an expression cloning strategy in Xenopus laevis oocytes, which likely plays role intestinal uptake calcium. CaT1 shows homology (75% amino acid sequence identity) to apical channel ECaC recently vitamin D-responsive cells rabbit kidney...
We report the phenotype of mice with targeted disruption Trpv6 (Trpv6 KO) epithelial calcium channel. The exhibit disordered Ca(2+) homeostasis, including defective intestinal absorption, increased urinary excretion, decreased BMD, deficient weight gain, and reduced fertility. Although our KO affects closely adjacent EphB6 gene, reported here is not related to dysfunction.
We examined the expression of calcium transporter 1 (CaT1) and epithelial channel (ECaC) mRNA in duodenum kidney mice. Intestinal CaT1 level increased 30-fold at weaning, coincident with induction calbindin-D(9k) expression. In contrast, renal ECaC was equal until weaning when is induced levels fall 70%. Long- short-term adaptation to changes dietary (Ca) 1,25 dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] injection strongly regulated duodenal calbindin D(9k) mRNA. Following a single dose...
To study the role of epithelial calcium channel transient receptor potential vanilloid type 6 (TRPV6) and calcium-binding protein calbindin-D9k in intestinal absorption, TRPV6 knockout (KO), KO, TRPV6/calbindin-D9k double-KO (DKO) mice were generated. DKO have serum levels similar to those wild-type (WT) (∼10 mg Ca2+/dl). In KO mice, however, there is a 1.8-fold increase PTH (P < 0.05 compared with WT). Active transport was measured using everted gut sac method. Under low dietary...
The extracellular calcium-sensing receptor (CaR; alternate gene names, CaR or Casr) is a membrane-spanning G protein–coupled receptor. highly expressed in the parathyroid gland, and activated by calcium (Ca2+o). Mice homozygous for null mutations (CaR–/–) die shortly after birth because of effects severe hyperparathyroidism hypercalcemia. A wide variety functions have been attributed to CaR. However, lethal CaR-deficient phenotype has made it difficult dissect direct effect deficiency from...
Active absorption of calcium from the intestine and reabsorption kidney are major determinants whole body homeostasis. Two recently cloned proteins, CaT1 ECaC, have been postulated to mediate apical uptake by rat rabbit kidney, respectively. By screening a cortex library with probe, we isolated cDNA encoding protein (CaT2) 84.2 73.4% amino acid identities ECaC CaT1, Unlike CaT2 is kidney-specific in was not detected intestine, brain, adrenal gland, heart, skeletal muscle, liver, lung,...
Yeast membrane proteins SMF1, SMF2, and SMF3 are homologues of the DCT1 metal ion transporter family. Their functional characteristics implications these <i>in vivo</i> have not yet been reported. Here we show that SMF1 expressed in <i>Xenopus</i> oocytes mediates H<sup>+</sup>-dependent Fe<sup>2+</sup> transport uncoupled Na<sup>+</sup> flux. SMF1-mediated Fe<sup>2+</sup>transport exhibited saturation kinetics (<i>K</i> <sub>m</sub> = 2.2 μm), whereas flux did not, although both processes...
Maternal-fetal calcium (Ca(2+)) transport is crucial for fetal Ca(2+) homeostasis and bone mineralization. In this study, the physiological significance of transient receptor potential, vanilloid 6 (TRPV6) channel in maternal-fetal was investigated using Trpv6 knockout mice. The concentration blood amniotic fluid significantly lower fetuses than wildtypes. activity radioactive ((45)Ca) from mother to 40% ash weight also compared with wildtype fetuses. TRPV6 mRNA protein were mainly localized...
The recently discovered apical calcium channels CaT1 (TRPV6) and ECaC (TRPV5) belong to a family of six members called the ‘TRPV family’. Unlike other four which are nonselective cation functioning as heat or osmolarity sensors in body, remarkably calcium-selective serve entry mechanisms absorptive secretory tissues. is highly expressed proximal intestine, placenta exocrine tissues, whereas expression most prominent distal convoluted connecting tubules kidney. intestine responsive...
The epithelial Ca(2+) channel TRPV5 serves as a gatekeeper for active reabsorption in the distal convoluted tubule and connecting of kidney. WNK4, protein serine/threonine kinase with gene mutations that cause familial hyperkalemic hypertension (FHH), including subtype hypercalciuria, is also localized nephron. To understand role WNK4 modulation reabsorption, we evaluated effect on TRPV5-mediated transport Xenopus laevis oocytes. Coexpression resulted twofold increase uptake. uptake was due...
Mutations in with‐no‐lysine (K) kinase 4 (WNK4) and a ubiquitin E3 ligase complex component kelch‐like 3 (KLHL3) both cause pseudohypoaldosteronism II (PHAII), hereditary form of hypertension. We determined whether WNK4 or its effector is regulated by KLHL3 Xenopus oocytes. inhibited the positive effect on Na + –Cl − cotransporter (NCC) decreasing protein abundance without that NCC downstream OSR1 directly. Ubiquitination degradation were induced KLHL3. The was blocked dominant negative...
Among the multiple organ disorders caused by severe acute respiratory syndrome coronavirus (SARS-CoV), lung failure following atypical pneumonia is most serious and often fatal event. We hypothesized that two of hydrophilic structural coronoviral proteins (S E) would regulate alveolar fluid clearance decreasing cell surface expression activity amiloride-sensitive epithelial sodium (Na(+)) channels (ENaC), rate-limiting protein in transepithelial Na(+) vectorial transport across distal cells....
The non-β endocrine cells in pancreatic islets play an essential counterpart and regulatory role to the insulin-producing β-cells regulation of blood-glucose homeostasis. While significant progress has been made towards understanding β-cell regeneration adults, very little is known about such as glucagon-producing α-cells somatostatin producing δ-cells. Previous studies have noted increase α-cell composition diabetes patients animal models. It thus our hypothesis that non-β-cells δ-cells...