A5062022173- Parathyroid Disorders and Treatments
- Ion Transport and Channel Regulation
- Magnesium in Health and Disease
- Ion channel regulation and function
- Neuroscience of respiration and sleep
- Neonatal Respiratory Health Research
- Regulation of Appetite and Obesity
- Erythrocyte Function and Pathophysiology
- Neuroscience and Neuropharmacology Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Asthma and respiratory diseases
- Bone health and treatments
- Congenital Diaphragmatic Hernia Studies
- Biochemical Analysis and Sensing Techniques
- Metabolism, Diabetes, and Cancer
- Bone health and osteoporosis research
- Iron Metabolism and Disorders
- Heavy Metal Exposure and Toxicity
- Biomedical Research and Pathophysiology
- Heme Oxygenase-1 and Carbon Monoxide
- Nutrition and Health in Aging
- Bone Metabolism and Diseases
- Renal function and acid-base balance
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Hemoglobin structure and function
Cardiff University
2014-2025
Medical University of Vienna
2020
King's College London
2020
Woolcock Institute of Medical Research
2020
The University of Sydney
2020
University of Manchester
1999-2020
Universidade de São Paulo
2015-2020
University of Oxford
2020
University of Copenhagen
2020
Monash University
2020
The maintenance of a stable extracellular concentration ionized calcium depends on the integrated function number specialized cells (e.g., parathyroid and certain kidney epithelial cells). We recently identified another G protein-coupled receptor (BoPCaRI) from bovine that responds to changes in Ca2+ within millimolar range provides key mechanism for regulating secretion hormone. Using an homology-based strategy, we now report isolation cDNA encoding Ca2+/polyvalent cation-sensing (RaKCaR)...
Modulation of calcium-sensitive potassium (BK) channels by oxygen is important in several mammalian tissues, and the carotid body it crucial to respiratory control. However, identity sensor remains unknown. We demonstrate that hemoxygenase-2 (HO-2) part BK channel complex enhances activity normoxia. Knockdown HO-2 expression reduced activity, carbon monoxide, a product rescued this loss function. Inhibition hypoxia was dependent on augmented stimulation. Furthermore, cells demonstrated...
We investigated the direct effects of changes in free ionized extracellular calcium concentrations ([Ca 2+ ] o ) on osteoblast function and involvement calcium-sensing receptor (CaR) mediating these responses. CaR mRNA protein were detected models, freshly isolated fetal rat calvarial cells murine clonal osteoblastic 2T3 cells, frozen, undecalcified preparations human mandible femur. In elevating [Ca treatment with gadolinium, a nonpermeant agonist, resulted phosphorylation signal-regulated...
We previously identified transcripts encoding a G protein-coupled, extracellular calcium/polyvalent cation-sensing receptor, RaKCaR, in rat kidney (D. Riccardi, J. Park, W.-S. Lee, G. Gamba, E. M. Brown, and S. C. Hebert. Proc. Natl. Acad. Sci. USA 92: 131–135, 1994), which was proposed to provide the mechanism for modulating variety of renal functions response changes Ca 2+ (E. Brown. In: Handbook Physiology. Bethesda, MD: Am. Physiol. Soc., 1992, sect. 8, vol. 2, chapt. 39, p. 1841–1916;...
Using a strategy based on homology to the bovine parathyroid Ca(2+)-sensing receptor previously identified by us (5), we have recently isolated an extracellular, G protein-coupled Ca2+/ polyvalent cation-sensing receptor, RaKCaR (22), from rat kidney. The localization and physiological role(s) of this in kidney are not well understood. In present study, assessed distribution mRNAs for hormone/parathyroid hormone-related protein (PTH/PTHrP) along nephron situ hybridization reverse...
The extracellular calcium ([Formula: see text])-sensing receptor (CaR) plays vital roles in [Formula: text] homeostasis, but no data are available on its expression small and large intestine. Polymerase chain reaction products amplified from reverse-transcribed duodenal RNA using CaR-specific primers showed >99% homology with the rat kidney CaR. Northern analysis a cRNA probe demonstrated 4.1- 7.5-kb transcripts all intestinal segments. Immunohistochemistry antisera clear basal staining...
Vascular calcification (VC) is highly correlated with increased morbidity and mortality in advanced chronic kidney disease (CKD) patients. Allosteric modulation of the calcium-sensing receptor (CaR) by calcimimetics inhibits VC animal models CKD. Here, we investigated expression CaR vasculature tested ability to prevent vascular smooth muscle cell (VSMC) vitro. Immunohistochemical staining demonstrated that protein present VSMC normal, non-calcified human arteries. In contrast, low levels...
Calcilytics reduce airway hyperresponsiveness and inflammation may represent effective asthma therapeutics.
Cachexia affects about 80 percent of gastrointestinal cancer patients. This multifactorial syndrome resulting in involuntary and continuous weight loss is accompanied by systemic inflammation immune cell infiltration various tissues. Understanding the interactions between tumor, cells peripheral tissues could help attenuating inflammation. Therefore, we investigated subcutaneous adipose tissue stable cachectic patients with same diagnosis, order to establish correlations tumor...
The balance between the concentrations of free ionized Ca2+ and bicarbonate in pancreatic juice is critical importance preventing formation calcium carbonate stones. How pancreas regulates ionic composition level saturation an alkaline environment such as not known. Because tight cause-effect relationship concentration lithogenicity, because hypercalcemia proposed etiologic factor for several diseases, we have investigated whether tissues express a Ca2+-sensing receptor (CaR) similar to that...
We have demonstrated that the kidney plays an important role in iron balance and metabolically significant reabsorption of this ion occurs loop Henle collecting ducts [Wareing M, Ferguson CJ, Green R, Riccardi D, Smith CP. J Physiol (Lond) 524: 581–586, 2000]. To test possibility divalent metal transporter DMT1 (Gunshin H, Mackenzie B, Berger UV, Gunshin Y, Romero MF, Boron WF, Nussberger S, Gollan JL, Hediger MA. Nature 388: 482–488, 1997) could represent apical route for entry kidney, we...
Thiazide diuretics are used worldwide as a first-choice drug for patients with uncomplicated hypertension. In addition to their antihypertensive effect, thiazides increase bone mineral density and reduce the prevalence of fractures. Traditionally, these effects have been attributed increased renal calcium reabsorption that occurs secondary inhibition thiazide-sensitive sodium chloride cotransporter (NCC) in distal tubule. The aim current study was determine whether exert direct bone-forming...