A5055233748- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Lipid Membrane Structure and Behavior
- Amino Acid Enzymes and Metabolism
- Neuroscience and Neuropharmacology Research
- Drug Transport and Resistance Mechanisms
- Protein Structure and Dynamics
- RNA modifications and cancer
- Molecular Sensors and Ion Detection
- Nanopore and Nanochannel Transport Studies
- Ion channel regulation and function
- Cancer, Hypoxia, and Metabolism
- Metabolism and Genetic Disorders
- Enzyme Structure and Function
- DNA and Nucleic Acid Chemistry
- Mass Spectrometry Techniques and Applications
- Ion Transport and Channel Regulation
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Advanced biosensing and bioanalysis techniques
- Biological Research and Disease Studies
- Metabolomics and Mass Spectrometry Studies
- Genetics, Bioinformatics, and Biomedical Research
University of Stuttgart
2016-2025
Ludwig-Maximilians-Universität München
1998-2005
Institut für Urheber- und Medienrecht
2002
Harvard University
1994-1997
Brigham and Women's Hospital
1994-1997
Harvard University Press
1994-1996
Yale University
1996
Czech Academy of Sciences, Institute of Analytical Chemistry
1996
Northeastern University
1996
Kyorin University
1994
Abstract The shape of biological matter is central to cell function at different length scales and determines how cellular components recognize, interact respond one another. However, their shapes are often transient hard reprogramme. Here we construct a synthetic model composed signal-responsive DNA nanorafts, biogenic pores giant unilamellar vesicles (GUVs). We demonstrate that reshaping rafts the nanoscale can be coupled GUVs microscale. nanorafts collectively undergo reversible...
Translocation of nuclear-encoded preproteins across the outer membrane mitochondria is mediated by multicomponent transmembrane TOM complex. We have isolated core complex Neurospora crassa removing receptors Tom70 and Tom20 from holo treatment with detergent dodecyl maltoside. It consists Tom40, Tom22, small Tom components, Tom6 Tom7. This was also purified directly after solubilization The has characteristics general insertion pore; it contains high-conductance channels binds preprotein in...
Active ion-coupled glutamate transport is of critical importance for excitatory synaptic transmission, normal cellular function, and epithelial amino acid metabolism. We previously reported the cloning rabbit intestinal high affinity transporter EAAC1 (Kanai, Y., Hediger, M. A.(1992) Nature 360, 467-471), which [Medline] expressed in numerous tissues including intestine, kidney, liver, heart, brain. Here, we report a detailed stoichiometric kinetic analysis Xenopus laevis oocytes. Uptake...
Tom40 is the main component of preprotein translocase outer membrane mitochondria (TOM complex). We have isolated Neurospora crassa by removing receptor Tom22 and small Tom components Tom6 Tom7 from purified TOM core complex. organized in a high molecular mass complex ∼350 kD. It forms conductance channel. Mitochondrial presequence peptides interact specifically with reconstituted into planar lipid membranes decrease ion flow through pores voltage-dependent manner. The secondary structure...
High affinity transport of glutamate across plasma membranes brain neurons and epithelia is mediated by a Na+-and K'-coupled electrogenic transporter.Here we report the primary structure functional characterization human high transporter (HEAACl).A unique characteristic HEAAC1mediated that for maximal rate are strongly dependent on membrane potential.Our data provide new insights into individual steps show mechanism distinct from y-aminobutyric acid GAT-1 Na+/glucose SGLTl.Under voltage...
Abstract Background Poly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival the cells absence suitable sources. Formation subcellular localization PHB was previously assumed to occur randomly cytoplasm accumulating bacteria. However, contradictionary results on Ralstonia eutropha were published, recently. Results Here, we provide evidence by transmission electron microscopy that localized close contact nucleoid...
Three-dimensional (3D) bioprinting promises to change future lifestyle and the way we think about aging, field of medicine, clinicians treat ailing patients. In this brief review, attempt give a glimpse into how recent developments in 3D are going impact vast research ranging from complex functional organ transplant toxicology studies printed organ-like spheroids. The techniques were successfully applied reconstructed tissue for implantation, application-based high-throughput (HTP) platforms...
Protein stability is a key factor in successful structural and biochemical research. However, the approaches for systematic comparison of protein are limited by sample consumption or compatibility with buffer components. Here we describe how miniaturized measurement intrinsic tryptophan fluorescence (NanoDSF assay) combination simplified description unfolding can be used to interrogate sample. We demonstrate that improved measures, such as apparent Gibbs free energy unfolding, rather than...
1. The intestinal H(+)-coupled peptide transporter PepT1, displays a broad substrate specificity and accepts most charged neutral di- tripeptides. To study the proton-to-peptide stoichiometry dependence of kinetic parameters on extracellular pH (pHo), rabbit PepT1 was expressed in Xenopus laevis oocytes used for uptake studies radiolabelled dipeptides, voltage-clamp analysis intracellular measurements. 2. did not display substrate-gated anion conductances that have been found to be...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpectroscopic characterization of three different monomeric forms the main chlorophyll a/b binding protein from chloroplast membranesStephan Nussberger, Jan P. Dekker, Werner Kuehlbrandt, Bauke M. van Bolhuis, Rienk Grondelle, and Herbert AmerongenCite this: Biochemistry 1994, 33, 49, 14775–14783Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December...
The intrinsic pathway of apoptotic cell death is mainly mediated by the BCL-2-associated X (BAX) protein through permeabilization mitochondrial outer membrane (MOM) and concomitant release cytochrome c into cytosol. In healthy, non-apoptotic cells, BAX predominantly localized in cytosol exhibits a dynamic shuttle cycle between mitochondria. Thus, initial association with mitochondria represents critical regulatory step enabling to insert MOMs, promoting ultimately resulting apoptosis....
The TOM complex is the main entry point for precursor proteins (preproteins) into mitochondria. Preproteins containing targeting sequences are recognized by and imported We have determined structure of core from
Precursor proteins of the solute carrier family and channel forming Tim components are imported into mitochondria in two main steps. First, they translocated through TOM complex outer membrane, a process assisted by Tim9/Tim10 complex. They passed on to TIM22 complex, which facilitates their insertion inner membrane. In present study, we have analyzed function translocation substrates across membrane mitochondria. The purified core was reconstituted lipid vesicles entrapped. precursor...
Ion-coupled solute transporters exhibit pre-steady-tate currents that resemble those of voltage-dependent ion channels. These were assumed to be mostly due binding and dissociation the coupling near extracellular transporter surface. Little attention was given analogous events may occur at intracellular To address this issue, we performed voltage clamp studies Xenopus oocytes expressing intestinal H+-coupled peptide cotransporter PepT1 recorded dependence transient charge movements in...
Abstract Control of extrasynaptic glutamate concentration in the central nervous system is an important determinant neurotransmission and excitotoxicity. Mechanisms that modulate transporter function are therefore critical factors these processes. The redox modulation uptake was examined by measuring transporter‐mediated electrical currents radiolabelled amino acid influx voltage‐clamped Xenopus oocytes expressing human neuronal EAAC1. Up down changes response to treatment with...
Transport of nuclear encoded proteins into mitochondria is mediated by multisubunit translocation machineries in the outer and inner membranes mitochondria. The TOM complex contains receptor pore components that facilitate recognition preproteins their transfer through membrane. In addition, a set small proteins. Tom7 Tom6 have been found Neurospora yeast, Tom5 has so far only latter organism. present study, we identified analyzed its function comparison to yeast Tom5, which proposed play...