A5055017522- Phosphodiesterase function and regulation
- Receptor Mechanisms and Signaling
- CAR-T cell therapy research
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Congenital heart defects research
- Endoplasmic Reticulum Stress and Disease
- Cancer Cells and Metastasis
- Phagocytosis and Immune Regulation
- Cholinesterase and Neurodegenerative Diseases
- Cancer Genomics and Diagnostics
- Biochemical Analysis and Sensing Techniques
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Biochemical and Molecular Research
- Ion Channels and Receptors
- FOXO transcription factor regulation
McMaster University
2022-2024
Discovery Centre
2024
Cleveland Clinic Lerner College of Medicine
2017-2023
Institute of Biophysics
2012-2022
Chinese Academy of Sciences
2012-2022
University of Chinese Academy of Sciences
2021-2022
Affiliated Hospital of Zunyi Medical College
2021
Zunyi Medical University
2021
Université Paris-Sud
2012-2014
Laboratoire d'Excellence en Recherche sur le Médicament et l'Innovation Thérapeutique
2012-2014
Genetic drivers of cancer can be dysregulated through epigenetic modifications DNA. Although the critical role DNA 5-methylcytosine (5mC) in regulation transcription is recognized, functions other non-canonical remain obscure. Here, we report identification novel N6-methyladenine (N6-mA) human tissues and implicate this mark disease, specifically highly malignant brain glioblastoma. Glioblastoma markedly upregulated N6-mA levels, which co-localized with heterochromatic histone modifications,...
Highlights•High pericyte coverage compromises chemotherapeutic efficacy in GBM patients•Targeting GSC-derived pericytes disrupts the blood-tumor barrier (BTB)•Inhibiting BMX kinase with ibrutinib and BTB•Ibrutinib enhances effusion activity of drugs poor BTB penetrationSummaryThe (BTB) is a major obstacle for drug delivery to malignant brain tumors such as glioblastoma (GBM). Disrupting therefore highly desirable but complicated by need maintain normal blood-brain (BBB). Here we show that...
The interplay between glioma stem cells (GSCs) and the tumor microenvironment plays crucial roles in promoting malignant growth of glioblastoma (GBM), most lethal brain tumor. However, molecular mechanisms underlying this crosstalk are incompletely understood. Here, we show that GSCs secrete Wnt-induced signaling protein 1 (WISP1) to facilitate a pro-tumor by survival both tumor-associated macrophages (TAMs). WISP1 is preferentially expressed secreted GSCs. Silencing markedly disrupts GSC...
Targeting DNA-PK induces glioma stem cell differentiation by destabilizing SOX2 to inhibit tumor growth in mouse glioblastoma models.
Article30 October 2020Open Access Transparent process SATB2 drives glioblastoma growth by recruiting CBP to promote FOXM1 expression in glioma stem cells Weiwei Tao Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA Search for more papers this author Aili Zhang Kui Zhai Zhi Huang Haidong Wenchao Zhou Qian Xiaoguang Fang orcid.org/0000-0002-3368-4133 Briana C Prager Division Regenerative Medicine, University California, San Diego, CA, Pathology, Case...
The epicardium promotes neovascularization and cardiomyocyte regeneration by generating vascular smooth muscle cells (SMCs) producing regenerative factors after adult heart infarction. It is therefore a potential cell resource for repair of the injured heart. However, also participates in fibrosis scarring heart, complicating its use medicine. In this study, we report coexpression TBX18 WT1 majority epicardial during mouse embryonic development. Furthermore, describe convenient chemically...
Bitter tastants can induce relaxation in precontracted airway smooth muscle by activating big-conductance potassium channels (BKs) or inactivating voltage-dependent L-type Ca2+ (VDLCCs). In this study, a new pathway for bitter tastant-induced was defined and investigated. We found nifedipine-insensitive tastant chloroquine-sensitive epithelium-denuded mouse tracheal rings (TRs) with acetylcholine (ACH). the presence of nifedipine (10 µM), ACH induced cytosolic elevation cell shortening...
Abstract Background The tumorigenic potential of glioma stem cells (GSCs) is associated with multiple reversible molecular alternations, but the role posttranslational protein sumoylation in GSCs has not been elucidated. development GSC-targeting drugs relies on discovery GSC-preferential modifications and relevant signaling pathways. In this work, we investigated status, major sumoylated substrate, key regulatory enzyme to explore therapeutic disrupting for glioblastoma (GBM) treatment....
Bitter taste receptors (TAS2Rs) are traditionally thought to be expressed exclusively on the buds of tongue. However, accumulating evidence has indicated that this receptor family performs non-gustatory functions outside mouth in addition taste. Here, we examined role TAS2Rs human and mouse detrusor smooth muscle (DSM). We showed mRNA for various TAS2R subtypes was both (DSM) at distinct levels. Chloroquine (CLQ), an agonist TAS2Rs, concentration-dependently relaxed carbachol- KCl-induced...
Brain metastases (BM) are mainly treated palliatively with an expected survival of less than 12 months after diagnosis. In many solid tumors, the human neural stem cell marker glycoprotein CD133 is a tumor-initiating population that contributes to therapy resistance, relapse, and metastasis.
The participation of large-conductance Ca2+ activated K+ channels (BKs) in chloroquine (chloro)-induced relaxation precontracted airway smooth muscle (ASM) is currently undefined. In this study we found that iberiotoxin (IbTx, a selective inhibitor BKs) and chloro both completely blocked spontaneous transient outward currents (STOCs) single mouse tracheal cells, which suggests might block BKs. We further inhibited sparks caffeine-induced global increases. Moreover, can directly BK from the...
This study examined the role of main vascular cAMP-hydrolysing phosphodiesterases (cAMP-PDE) in regulation basal tone and relaxation rat aorta mediated by β-adrenoceptors, following heart failure (HF).Twenty-two weeks after proximal aortic stenosis, to induce HF, or SHAM surgery rats, we evaluated expression, activity function cAMP-PDE descending thoracic aorta.HF aortas exhibited signs endothelial dysfunction, with alterations NO pathway, alteration PDE3 PDE4 subtype without changing total...
We investigated the role of cyclic nucleotide phosphodiesterases (PDEs) in spatiotemporal control intracellular cAMP concentrations rat aortic smooth muscle cells (RASMCs).
ABSTRACT The stretching of smooth muscle tissue modulates contraction through augmentation Ca2+ transients, but the mechanism underlying stretch-induced transients is still unknown. We found that mechanical and maintenance mouse urinary bladder strips single myocytes at 30% 18% beyond initial length, respectively, resulted in oscillations. Experiments indicated remarkably increased production nitric oxide (NO) as well amplitude duration contraction. Stretch-induced oscillations contractility...
<div>AbstractPurpose:<p>Brain metastases (BM) are mainly treated palliatively with an expected survival of less than 12 months after diagnosis. In many solid tumors, the human neural stem cell marker glycoprotein CD133 is a tumor-initiating population that contributes to therapy resistance, relapse, and metastasis.</p>Experimental Design:<p>Here, we use variant our previously described binder generate second-generation CD133-specific chimeric antigen receptor T cells...
Chrysin (5,7-dihydroxyflavone) is a natural flavone commonly found in many plants including PASSIFLORA COERULEA L. Researchers have performed extensive and detailed investigations on the behavioral pharmacological effects of chrysin IN VIVO, but there was little information available effect nociception. Therefore, present study undertaken to investigate nociceptive threshold using tail-immersion test. Intraperitoneal ( I. P.) injection (10, 25, 50, 75, 100 mg/kg) dose- time-dependently...