A5075930863- Spinal Cord Injury Research
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- CAR-T cell therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Spinal Dysraphism and Malformations
- Glioma Diagnosis and Treatment
- Spinal Hematomas and Complications
- Axon Guidance and Neuronal Signaling
- Stress Responses and Cortisol
- Cancer Cells and Metastasis
- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced Neuroimaging Techniques and Applications
- Immune cells in cancer
- Organ Donation and Transplantation
- Tryptophan and brain disorders
- Advancements in Semiconductor Devices and Circuit Design
- Neuroscience of respiration and sleep
- Immune Cell Function and Interaction
- Prion Diseases and Protein Misfolding
- Nerve Injury and Rehabilitation
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Liver Disease Diagnosis and Treatment
- Microbial infections and disease research
McMaster University
2015-2024
Medical University of Lublin
2015-2019
St. Joseph’s Healthcare Hamilton
2019
Concern Worldwide US
2019
Hamilton Health Sciences
2011
University of Wisconsin–Madison
1997-1999
University of Guelph
1992-1995
Engineering T cells with chimeric antigen receptors (CARs) is an effective method for directing to attack tumors, but may cause adverse side effects such as the potentially lethal cytokine release syndrome. Here authors show that cell coupler (TAC), a receptor co-opts endogenous TCR, induces more efficient anti-tumor responses and reduced toxicity when compared past-generation CARs. TAC-engineered induce robust antigen-specific production cytotoxicity in vitro, strong activity variety of...
The pathogenesis of spinal cord injury (SCI) remains poorly understood and treatment limited. Emerging evidence indicates that post-SCI inflammation is severe but the role reactive astrogliosis not well given its implication in ongoing as damaging or neuroprotective. We have completed an extensive systematic study with MRI, histopathology, proteomics ELISA analyses designed to further define protracted after SCI a rat model. identified 3 distinct phases SCI: acute (first 2 days),...
Ligands for the NKG2D receptor are overexpressed on tumors, making them interesting immunotherapy targets. To assess tumoricidal properties of T cells directed to attack ligands, we engineered murine with two distinct NKG2D-based chimeric antigen receptors (CARs): (i) a fusion between and CD3ζ chain (ii) conventional second-generation CAR, where extracellular domain was fused CD28 CD3ζ. enhance CAR surface expression, also coexpress DAP10. In vitro functionality expression levels all three...
Medulloblastoma (MB), the most common malignant paediatric brain tumor, is currently treated using a combination of surgery, craniospinal radiotherapy and chemotherapy. Owing to MB stem cells (MBSCs), subset patients remains untreatable despite standard therapy. CD133 used identify MBSCs although its functional role in tumorigenesis has yet be determined. In this work, we showed enrichment Group 3 associated with increased rate metastasis poor clinical outcome. The signal transducers...
The master regulatory gene Bmi1 modulates key stem cell properties in neural precursor cells (NPCs), and has been implicated brain tumorigenesis. We previously identified a population of CD133+ tumor possessing properties, known as initiating (BTICs). Here, we characterize the expression role primary minimally cultured human glioblastoma (GBM) patient isolates CD133- sorted populations. find that is increased cells, protein transcript are highest during intermediate stages differentiation...
Increased liver de novo lipogenesis (DNL) is a hallmark of nonalcoholic steatohepatitis (NASH). A key enzyme controlling DNL upregulated in NASH ATP citrate lyase (ACLY). In mice, inhibition ACLY reduces steatosis, ballooning, and fibrosis inhibits activation hepatic stellate cells. Glucagon-like peptide-1 receptor (GLP-1R) agonists lower body mass, insulin resistance, steatosis without improving fibrosis. Here, we find that combining an inhibitor ACLY, bempedoic acid, the GLP-1R agonist...
Abstract Myelin‐derived molecules inhibit axonal regeneration in the CNS. The Long–Evans Shaker rat is a naturally occurring dysmyelinated mutant, which although able to express components of myelin lacks functional adulthood. Given that breakdown exposes axons are inhibitory regeneration, we sought determine whether injured dorsal column would exhibit regenerative response absent normally myelinated (control) rats. Although did not regenerate beyond lesion, they remained at caudal end crush...
Creatine monohydrate (CrM) supplementation appears to be relatively safe based on data from short-term and intermediate-term human studies results several therapeutic trials. The purpose of the current study was characterize pathological changes after long-term CrM in mice [healthy control SOD1 (G93A) transgenic] rats (prednisolone nonprednisolone treated). Histological assessment (18-20 organs/tissues) performed G93A 159 days, Sprague-Dawley 365 (2% wt/wt) compared with feed. Liver...
Abstract Triple-negative breast cancers (TNBCs) represent a subset of tumors that are highly aggressive and metastatic, responsible for disproportionate number cancer-related deaths. Several studies have postulated role the epithelial-to-mesenchymal transition (EMT) program in increased aggressiveness metastatic propensity TNBCs. Although EMT is essential early vertebrate development wound healing, it frequently co-opted by cancer cells during tumorigenesis. One prominent signaling pathway...
Spinal cord injury (SCI) results in massive secondary damage characterized by a prolonged inflammation with phagocytic macrophage invasion and tissue destruction. In prior work, sustained subdural infusion of anti-inflammatory compounds reduced neurological deficits pro-inflammatory cell at the site leading to improved outcomes. We hypothesized that implantation hydrogel loaded an immune modulating biologic drug, Serp-1, for delivery after crush-induced SCI would have effective...
Our understanding of myelination has been greatly enhanced via the study spontaneous mutants that harbor a defect in gene encoding one major myelin proteins (myelin mutants). In this study, we describe unique genetic new mutant called Long Evans shaker ( les ) rat causes severe dysmyelination CNS. Myelin deficits result from disruption basic protein Mbp caused by insertion an endogenous retrotransposon [early transposons (ETn) element] into noncoding region (intron 3) gene. The ETn element...
Brain metastases (BM) are mainly treated palliatively with an expected survival of less than 12 months after diagnosis. In many solid tumors, the human neural stem cell marker glycoprotein CD133 is a tumor-initiating population that contributes to therapy resistance, relapse, and metastasis.
AMA Kwiecien J, Jarosz B, Urdzikova L, Rola R, Dabrowski W. Original articleSubdural infusion of dexamethasone inhibits leukomyelitis after acute spinal cord injury in a rat model. Folia Neuropathologica. 2015;53(1):41-51. doi:10.5114/fn.2015.49973. APA Kwiecien, J., Jarosz, B., Urdzikova, L., Rola, R., & Dabrowski, (2015). Neuropathologica, 53(1), 41-51. https://doi.org/10.5114/fn.2015.49973 Chicago Jacek M., Bozena Lucia Machova Radoslaw and Wojciech Dabrowski. 2015. "Original model"....
ABSTRACT Streptococcus pneumoniae is a leading cause of invasive bacterial infections, with nasal colonization an important first step in disease. While cigarette smoking strong risk factor for pneumococcal disease, the underlying mechanisms remain unknown. This partly due to lack clinically relevant animal models investigating context smoke exposure. We present model smoke-exposed mice and document, time, that predisposes infection mortality model. Cigarette increased bacteremia meningitis...
The goal of this study was to determine the contribution distal nerve sheath sensory protection. Following tibial transection, rats were assigned one following groups: (1) saphenous-to-tibial neurorrhaphy; (2) saphenous-to-gastrocnemius neurotization; (3) unprotected controls (tibial transection); or (4) immediate common peroneal-to-tibial neurorrhaphy. After a 6-month denervation period and motor reinnervation, ultrastructural, histologic, morphometric analyses performed on gastrocnemius...
AMA Kwiecien J. Original articleCellular mechanisms of white matter regeneration in an adult dysmyelinated rat model. Folia Neuropathologica. 2013;51(3):189-202. doi:10.5114/fn.2013.37703. APA Kwiecien, (2013). Neuropathologica, 51(3), 189-202. https://doi.org/10.5114/fn.2013.37703 Chicago Jacek M. 2013. "Original model". Neuropathologica 51 (3): Harvard pp.189-202. MLA model." vol. 51, no. 3, 2013, pp. Vancouver