A5038218325- Meningioma and schwannoma management
- Glioma Diagnosis and Treatment
- Ion Transport and Channel Regulation
- Traumatic Brain Injury and Neurovascular Disturbances
- Intracranial Aneurysms: Treatment and Complications
- Pituitary Gland Disorders and Treatments
- Head and Neck Surgical Oncology
- Sepsis Diagnosis and Treatment
- Cerebrospinal fluid and hydrocephalus
- Ion channel regulation and function
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Electrolyte and hormonal disorders
- Neuroscience and Neuropharmacology Research
- Moyamoya disease diagnosis and treatment
- Intracerebral and Subarachnoid Hemorrhage Research
- Cerebrovascular and Carotid Artery Diseases
- Spinal Dysraphism and Malformations
- Traumatic Brain Injury Research
- Acute Ischemic Stroke Management
- Chronic Disease Management Strategies
- Cancer Genomics and Diagnostics
- Facial Trauma and Fracture Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Machine Learning in Healthcare
McMaster University
2022-2024
St. Michael's Hospital
2018-2024
Deleted Institution
2022
University of Toronto
2018-2022
St Michael’s Hospital
2019
Salford Royal NHS Foundation Trust
2013-2018
University of Manchester
2016-2018
University of California, San Francisco
2006-2017
Royal Manchester Children's Hospital
2017
Farr Institute
2017
Abstract The glial water channel aquaporin‐4 (AQP4) has been hypothesized to modulate and potassium fluxes associated with neuronal activity. In this study, we examined the seizure phenotype of AQP4 −/− mice using in vivo electrical stimulation electroencephalographic (EEG) recording. were found have dramatically prolonged stimulation‐evoked seizures after hippocampal compared wild‐type controls (33 ± 2 s vs. 13 s). addition, a higher threshold (167 17 μA 114 10 μA). To assess potential...
OBJECTIVE Bypass surgery for brain aneurysms is evolving from extracranial-intracranial (EC-IC) to intracranial-intracranial (IC-IC) bypasses that reanastomose parent arteries, revascularize efferent branches with in situ donor arteries or reimplantation, and reconstruct bifurcated anatomy grafts are entirely intracranial. We compared results these newer IC-IC conventional EC-IC bypasses. METHODS During a 10-year period, 82 patients underwent bypass as part of their aneurysm management. A...
Aquaporin-4 (AQP4) is a water transport protein expressed in glial cell plasma membranes, including foot processes lining the blood-brain barrier. AQP4 deletion mice reduces cytotoxic brain edema produced by different pathologies. To determine whether rate-limiting for accumulation and altered expression, as occurs various pathologies, could have functional importance, we generated that overexpressed cells transgenic approach using fibrillary acid promoter. Overexpression of ∼2.3-fold did...
Tumor-associated macrophages (TAMs) constitute up to 50% of tumor bulk in glioblastoma (GBM) and play an important role maintenance progression. The recently discovered differences between invading tumour periphery hypoxic core implies that macrophage biology is also distinct by location. This may provide further insight into the observed treatment resistance immune modulation. We hypothesize activation occurs through processes are versus core. therefore investigated regional TAM recruitment...
The treatment of dolichoectatic basilar trunk aneurysms has been ineffectual or morbid due to nonsaccular morphology, deep location, and involvement brainstem perforators. Treatment with bypass surgery advocated eliminate malignant hemodynamics stabilize aneurysm growth.
Aquaporin-4 (AQP4) is a water-selective transport protein expressed in glial cells throughout the central nervous system. AQP4 deletion mice produces alterations several neuroexcitation phenomena, including hearing, vision, epilepsy, and cortical spreading depression. Here, we report defective olfaction electroolfactogram responses AQP4-null mice. Immunofluorescence indicated strong expression supportive of nasal olfactory epithelium. The epithelium had identical appearance, but did not...
Brain edema following traumatic brain injury (TBI) is associated with considerable morbidity and mortality. Prior indirect evidence has suggested the involvement of astrocyte water channel aquaporin-4 (AQP4) in pathogenesis TBI. Here, focal TBI was produced wild type (AQP4+/+) knockout (AQP4−/−) mice by controlled cortical impact (CCI) craniotomy dura intact (parameters: velocity 4.5 m/sec, depth 1.7 mm, dwell time 150 msec). AQP4-deficient showed a small but significant reduction volume...
Sepsis remains a complex medical problem and major challenge in healthcare. Diagnostics outcome predictions are focused on physiological parameters with less consideration given to patients' background. Given the aging population, not only diseases becoming increasingly prevalent but occur more frequently combinations ("multimorbidity"). We hypothesized existence of patient subgroups critical care distinct multimorbidity states. further hypothesize that certain states associate higher rates...
Cerebral edema contributes significantly to morbidity and mortality after brain injury stroke. Aquaporin-4 (AQP4), a water channel expressed in astrocytes, plays key role homeostasis. Genetic variants other aquaporin family members have been associated with disease phenotypes. However, human AQP4, only one non-synonymous single-nucleotide polymorphism (nsSNP) has reported, no characterization of protein function or phenotype. We analyzed DNA from an ethnically diverse cohort 188 individuals...
Seizures are important neurological complications after traumatic brain injury (TBI) and reported for up to 50% of patients with TBI. Despite several studies, no drug strategy has been able alter the biological events leading epileptogenesis. The glial water channel, aquaporin-4 (AQP4), was shown facilitate cytotoxic cell swelling in ischemia scar formation stab wound injury. In this study, we examined post-traumatic seizure susceptibility AQP4-deficient mice (AQP4–/–) injection...
BACKGROUND: Rotational vertebral artery syndrome (RVAS) is a rare entity about which previously published studies are mostly limited to individual case reports. OBJECTIVE: To report our decade-long experience with this in 9 patients compression ranging from the occiput C6. METHODS: We utilized posterior approach for lesions rostral C4 and an anterior at or caudal C4. Furthermore, we demonstrated feasibility efficacy of minimally invasive cervical approach. Patient profile, operative...
Traumatic brain injury remains a global health problem. Understanding the relative importance of outcome predictors helps optimize our treatment strategies by informing assessment protocols, clinical decisions and trial designs. In this study we establish ranking for based on receiver operating indices to identify key create simple predictive models. We then explore associations between using Bayesian networks gain further insight into predictor importance.We analyzed corticosteroid...
Abstract Traumatic brain injury (TBI) is a complex condition where heterogeneity impedes the advancement of care. Understanding diverse presentations TBI crucial for personalized medicine. Our study aimed to identify clinically relevant patient endotypes in using latent class analysis based on comorbidity data. We used Medical Information Mart Intensive Care III database, which includes 2,629 adult patients. identified five stable characterized by specific profiles: Heart Failure and...
OBJECTIVE Monitoring of brain tissue oxygen tension is increasingly being used to monitor patients after severe traumatic injury and guide therapies aimed at maintaining above threshold levels. The new Licox PMO combined temperature catheter (Integra LifeSciences, Plainsboro, NJ) combines measurements in a single probe inserted through bolt mechanism. In this study, we sought evaluate the accuracy under controlled laboratory conditions assess automated card calibration system. We also...
The subtemporal approach for a superficial temporal artery-to-superior cerebellar artery bypass requires significant superior retraction that can injure the lobe, compromise veins, and cause edema postoperatively. In contrast, pretemporal posterolateral seems to be less injurious lobe better tolerated clinically. We hypothesized provides ample exposure, more gentle retraction, clinical results than approach.Standard orbitozygomatic-pterional approaches were performed on both sides of 4...
Diffusion in brain extracellular space (ECS) is important for nonsynaptic intercellular communication, ionic buffering, and delivery of drugs metabolites. We measured macromolecular diffusion normally light-inaccessible regions mouse by microfiberoptic epifluorescence photobleaching, which a fiberoptic with micron-size tip introduced deep tissue. In cortex, the noninteracting molecule [fluorescein isothiocyanate (FITC)-dextran, 70 kDa] was slowed 4.5 +/- 0.5-fold compared its water (D(o)/D),...
The astrocyte water channel aquaporin-4 (AQP4) regulates extracellular space (ECS) K+ concentration ([K+]e) and volume dynamics following neuronal activation. Here, we investigated how AQP4-mediated changes in [K+]e ECS affect the velocity, frequency, amplitude of cortical spreading depression (CSD) depolarizations produced by surface KCl application wild-type (AQP4+/+) AQP4-deficient (AQP4−/−) mice. In contrast to initial expectations, both velocity frequency CSD were significantly reduced...
Early surgical intervention to decompress the spinal cord and stabilize column in patients with acute traumatic thoracolumbar injury (TLSCI) may lessen risk of developing complications improve outcomes. However, there has yet be agreement on what constitutes "early" surgery; reported thresholds range from 8 72 h. To address this knowledge gap, we conducted an observational cohort study using data American College Surgeons (ACS) Trauma Quality Improvement Program (TQIP) 2010 2016. The...