A5079705755- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Phosphodiesterase function and regulation
- Pulmonary Hypertension Research and Treatments
- Ion channel regulation and function
- Eicosanoids and Hypertension Pharmacology
- Heart Failure Treatment and Management
- Renin-Angiotensin System Studies
- Heart Rate Variability and Autonomic Control
- Asthma and respiratory diseases
- Protein Kinase Regulation and GTPase Signaling
- Adipose Tissue and Metabolism
- Cystic Fibrosis Research Advances
- Traditional and Medicinal Uses of Annonaceae
- Ethnobotanical and Medicinal Plants Studies
- Neonatal Respiratory Health Research
- Pharmacological Effects and Assays
- Phytochemistry and Bioactive Compounds
- Natural Antidiabetic Agents Studies
- African Botany and Ecology Studies
- Sirtuins and Resveratrol in Medicine
- Botanical Research and Chemistry
- Alcohol Consumption and Health Effects
- Sphingolipid Metabolism and Signaling
- Medicinal plant effects and applications
Inserm
2015-2024
Université Paris-Saclay
2016-2024
Laboratory Signaling and Cardiovascular Pathophysiology
2017-2024
Université Paris-Sud
2011-2020
Institut Galien Paris-Saclay
2011-2016
Laboratoire d'Excellence en Recherche sur le Médicament et l'Innovation Thérapeutique
2011-2014
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2011-2013
Sorbonne Université
2012
Université de Bordeaux
2004-2011
Centre de Recherche Cardio-Thoracique de Bordeaux
2011
Beta1- and beta2-adrenoceptors in heart muscle cells mediate the catecholamine-induced increase force frequency of cardiac contraction. Recently, addition, we demonstrated functional expression beta3-adrenoceptors human heart. Their stimulation, marked contrast with that beta1- beta2-adrenoceptors, induces a decrease contractility through presently unknown mechanisms. In present study, examined role nitric oxide (NO) synthase pathway mediating beta3-adrenoceptor effect on endomyocardial...
The relaxant effects of isoprenaline may result from activation another β‐adrenoceptor subtype in addition to β 1 and 2 . This study evaluated the role a third subtype, 3 , β‐adrenoceptor‐induced relaxation rat thoracic aorta by isoprenaline. Isoprenaline produced concentration‐dependent phenylephrine pre‐contracted rings (pD =7.46±0.15; E max =85.9±3.4%), which was partially attenuated endothelium removal (E =66.5±6.3%) administration nitric oxide (NO) synthase inhibitor, L ‐N G ‐monomethyl...
This study investigates the role of nitric oxide (NO) and reactive oxygen species (ROS) on endothelial function pulmonary arteries in a mice model hypoxia‐induced hypertension. In from control mice, NO‐synthase inhibitor N ω ‐nitro‐ L ‐arginine methyl ester ( ‐NAME) potentiated contraction to prostaglandin F 2 α (PGF ) completely abolished relaxation acetylcholine. extrapulmonary but not intrapulmonary arteries, acetylcholine‐induced was slightly inhibited by polyethyleneglycol‐superoxide...
Compartmentation of intracellular signaling pathways serves as an important mechanism conferring the specificity G protein-coupled receptor (GPCR) signaling. In heart, stimulation β 2 -adrenoceptor (β -AR), a prototypical GPCR, activates tightly localized protein kinase A (PKA) signaling, which regulates substrates at cell surface membranes, bypassing cytosolic target proteins (eg, phospholamban). Although concurrent activation -AR-coupled i has been implicated in functional compartmentation...
Because pulmonary circulation is the primary vascular target of inhaled particulate matter (PM), and nitric oxide a major vasculoprotective agent, in this study we investigated effect various particles on NO-cyclic guanosine monophosphate (cGMP) pathway arteries.We used intrapulmonary arteries and/or endothelial cells, either exposed vitro to or removed from PM-instilled animals for assessment vasomotricity, cGMP reactive oxygen species (ROS) levels, cytokine/chemokine release.Endothelial...
β-adrenoceptor (β-AR)-mediated relaxation was characterized in pulmonary arteries from normoxic and hypoxic (as model of hypertension) mice. The endothelial NO synthase (eNOS) pathway especially investigated because its potential vasculoprotective effects. Pulmonary control or (0.5 atm for 21 days) wild-type eNOS−/− mice were used pharmacological characterization β-AR-mediated myograph, immunohistochemistry using anti-β-AR antibodies. In mice, isoproterenol (β-AR agonist) procaterol...
Phosphoinositide 3-kinase (PI3K) has been implicated in beta2-adrenergic receptor (beta2-AR)/G(i)-mediated compartmentation of the concurrent G(s)-cAMP signaling, negating beta2-AR-induced phospholamban phosphorylation and positive inotropic lusitropic responses cardiomyocytes. However, it is unclear whether PI3K crosstalks with beta1-AR signal transduction, even more generally, cAMP/PKA pathway. In this study, we show that selective stimulation markedly increases activity adult rat...
This study investigates the role of cyclooxygenase (COX)/prostanoid pathway in chronic hypoxia-induced hyperreactivity pulmonary arteries. Pulmonary arteries were removed from normoxic or hypoxic (0.5 atm for 21 days) mice and studied protein expression/localization COX-1, COX-2, thromboxane A2 (TXA2)-synthase, release TXA2, prostacyclin (PGI2) isoprostane 8-iso-prostaglandin F2α (8-iso-PGF2α), vasomotor responses. COX-2 expression was increased all layers mice. In contrast, COX-1 not...
Up-regulation of phosphodiesterases (PDEs) is associated with several vascular diseases, and better understanding the roles each PDE isoform in controlling subcellular pools cyclic nucleotides cells needed. We investigated respective role PDE1, PDE5, PDE9 intracellular cAMP and/or cGMP concentrations ([cAMP]i , [cGMP]i ) cultured rat aortic smooth muscle (RASMCs).We used selective inhibitors PDE1 (PF-04471141), PDE5 (sildenafil), (PF-04447943) to measure cAMP- cGMP-PDE activities a...
We investigated whether high levels of expression the cystic fibrosis transmembrane conductance regulator (CFTR) would alter functional properties newly synthesized recombinant proteins. COS-7, CFPAC-1, and A549 cells were intranuclearly injected with a Simian virus 40-driven pECE-CFTR plasmid assayed for halide permeability using 6-methoxy- N-(3-sulfopropyl)quinolinium fluorescent probe. With increasing numbers microinjected copies, baseline to dose dependently increased, response adenosine...
This study investigates the effect of aryloxypropanolamines 4-[3-[(1,1-dimethylethyl)amino]-2-hydroxypropoxy]-1,3-dihydro-2<i>H</i>-benzimidazol-2-one (CGP 12177), bupranolol, and 3-(2-ethylphenoxy)-1[(1<i>S</i>)-1,2,3,4-tetrahydronaphth-1-ylamino]-(2<i>S</i>)-2-propanol oxalate (SR 59230A) [commonly used as β<sub>3</sub>- and/or atypical β-adrenergic receptors (β-AR) ligands] on contractile function rat intralobar pulmonary artery. Affinities β-AR ligands for α<sub>1</sub>-adrenergic...
This study examined the role of main vascular cAMP-hydrolysing phosphodiesterases (cAMP-PDE) in regulation basal tone and relaxation rat aorta mediated by β-adrenoceptors, following heart failure (HF).Twenty-two weeks after proximal aortic stenosis, to induce HF, or SHAM surgery rats, we evaluated expression, activity function cAMP-PDE descending thoracic aorta.HF aortas exhibited signs endothelial dysfunction, with alterations NO pathway, alteration PDE3 PDE4 subtype without changing total...
In human cardiac myocytes, we have previously identified a functional β3-adrenoceptor in which stimulation reduces action potential duration. Surprisingly, biopsies obtained from cystic fibrosis patients, agonists produced no effects on This result suggests the involvement of transmembrane conductance regulator (CFTR) chloride current electrophysiological non-cystic tissues. We therefore investigated control CFTR activity by β3-adrenoceptors recombinant system: A549 cells were intranuclearly...
We investigated the role of cyclic nucleotide phosphodiesterases (PDEs) in spatiotemporal control intracellular cAMP concentrations rat aortic smooth muscle cells (RASMCs).