A5056252246- MicroRNA in disease regulation
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer-related molecular mechanisms research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Genetic and Kidney Cyst Diseases
- Epigenetics and DNA Methylation
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Advanced Radiotherapy Techniques
- Cancer Immunotherapy and Biomarkers
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Renal cell carcinoma treatment
- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- Cancer Diagnosis and Treatment
- Immune Cell Function and Interaction
- Genomics, phytochemicals, and oxidative stress
- Viral-associated cancers and disorders
- Vascular Tumors and Angiosarcomas
Eastern Hepatobiliary Surgery Hospital
2013-2025
Second Military Medical University
2014-2025
John Wiley & Sons (United States)
2016
Sustained endoplasmic reticulum (ER) stress has been linked to cell death and the pathogenesis of many liver diseases, including toxic liver, cholestasis, infectious disease. The cellular pathways that attenuate hepatic ER have focus recent studies, but role microRNAs (miRNA) in this process remains unknown. Here, we report one most abundant miRNAs hepatocytes, miR-199a-5p, was elevated both bile acid- thapsigargin (TG)-stimulated cultured as well duct-ligated mice. We identify misfolded...
Significance Hepatocellular carcinoma (HCC) is the sixth most common type of cancer, and its mortality rate continues to increase. We generated knock-in reporter mouse models for measuring DNA double-strand break (DSB) repair efficiency demonstrated that both DSB pathways are up-regulated in HCCs. then found activation PARP1 DNA-PKcs critical HCC survival vitro vivo. Targeting two proteins block by combining olaparib NU7441 synergistically inhibits growth orthotopic human PDX models. Our...
In recent years, circular RNAs (circRNAs) have been shown to critical regulatory roles in the resistance anti-cancer drugs. However, contributions of circRNAs sorafenib hepatocellular carcinoma (HCC) remain largely unknown. The present study aims explore involvement circFN1 and how is associated with miR-1205/E2F1 pathway, which demonstrated mediate this HCC cells. We investigated expression five paired sorafenib-sensitive HepG2 cells sorafenib-resistant (SR)-HepG2 by microarray analysis....
Background Microvascular invasion (MVI) is linked to poor prognosis, early recurrence and post-surgical intrahepatic metastasis of hepatocellular carcinoma (HCC) but roles tumor-associated endothelial cells (TECs) remain unclear. The aim the current study was investigate role TECs in microvascular HCC. Methods Single-cell RNA sequencing (scRNA-seq) data from three patients with MVI two non-MVI HCC were used identify subpopulations via Seurat R package. Using bioinformatics analysis...
Abstract The current study elucidated the role of a long non‐coding RNA (lncRNA), FOXD2‐AS1, in pathogenesis hepatocellular carcinoma (HCC) and regulatory mechanism underlying FOXD2‐AS1/miR‐150‐5p/transmembrane protein 9 (TMEM9) signalling HCC. Microarray analysis was used for preliminary screening candidate lncRNAs HCC tissues. qRT‐PCR Western blot analyses were to detect expression FOXD2‐AS1. Cell proliferation assays, luciferase assay immunoprecipitation performed examine by which...
Abstract Background Dysregulation of long non-coding RNAs (lncRNAs) results in development human diseases including hepatocellular carcinoma (HCC). Although several HCC related lncRNAs have been reported, the biological functions many during remains unknown. Methods The expression ST8SIA6-AS1 was studied by realtime PCR (RT-qPCR) and bioinformatic analysis. examined CCK-8 assay flow cytometry target analyzed analysis validated dual luciferase reporter assay, western blotting RT-qPCR. Results...
BACKGROUND Genomic alterations of small bowel cancers remain poorly understood due to the rarity these diseases. In current study, authors report identification somatic mutations from patients with duodenal adenocarcinoma by whole‐exome sequencing. METHODS Whole‐exome sequencing and follow‐up analysis were conducted in 12 matched tumor‐normal tissue pairs examine genetic characteristics this disease. Somatic (single‐nucleotide variants short insertion/deletions) obtained filtered then...
Primary hepatic neuroendocrine neoplasms (PHNENs) represent a kind of rare liver tumor and its clinical features prognosis remain unclear. This study aims to reveal the long-term therapeutic outcome PHNEN present prognostic feature. A retrospective designed, single-center containing 22 patients with PHNENs receiving surgical resections was done. Clinical data were reviewed follow-up updated. Survival analysis tried find factors. Nine recurred (recurrence rate = 40.9%) 6 died on disease. The...
Density of tumor infiltrating lymphocytes (TIL) and expressions certain immune-related genes have prognostic predictive values in hepatocellular carcinoma (HCC); however, factors determining the immunophenotype HCC patients are still unclear. In current study, transcript sequencing data liver cancer were systematically analyzed to determine an immune gene marker for prediction clinical outcome HCC.RNASeq follow-up information downloaded from The Cancer Genome Atlas (TCGA), samples assigned...
Hepatocellular carcinoma (HCC) is the seventh most common type of cancer; notably, incidence HCC four to eight times higher in men than women. Previous studies reported that estrogen receptor (ER) signaling pathway involved pathogenesis HCC, although extent its involvement unclear due several conflicting reports. In present study, tumor and paired adjacent non-cancerous tissues from 157 patients were collected. Transcriptome sequencing real-time quantitative polymerase chain reaction used...
Hepatocellular carcinoma (HCC) is the fifth most common cancer in world with poor prognosis. Here, we investigated role of microRNA 218 (miR-218) regulating human HCC development. Quantitative PCR (qPCR) was used to compare expression levels miR-218 between eight and a normal liver cell lines, as well nine primary tissues adjacent non-carcinoma tissues. lines MHCC97L Huh7 were transfected lentiviral vector mimics. The effect overexpression on growth, both vitro vivo, invasion examined. A...
Hepatic ischemia-reperfusion injury (IRI) is a very complex pathological process that often associated with liver trauma and surgery, especially transplantation surgery. Although endoplasmic reticulum stress (ERS) plays role in this process, the posttranscriptional regulators underlying mechanisms are still unclear. Here, we report lncRNA AK054386 was increased hepatic IRI models. Furthermore, can act as "competing endogenous RNA (ceRNA)" regulate ERS-related factors by binding sequestering...
Abstract Background Hepatocellular carcinoma (HCC) is a malignancy with poor prognosis. Complex genetic and epigenetic alterations are the two primary causes of HCC. The aim study was mainly to explore correlation between methylation status DNAH17 Methods We evaluated levels in 163 HCC samples their paired normal tissue using Sequenom EpiTYPER assays performed TaqMan copy number assay assess samples. Results mean were significantly decreased tumor tissues compared both selected regions...
Thousands of long non-coding RNAs (lncRNAs) have been functionally verified as crucial regulators physiological processes and disease progressions, yet their roles in hepatocellular carcinoma (HCC) not clearly illuminated.We analyzed the expression lncRNA-SNHG14 TCGA data via bioinformatic analysis detected its HCC specimens by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Loss-of-function experiments were used to study biological function SNHG14 cells. RT-qPCR,...
Aberrant methylation is a hallmark of hepatocellular carcinoma and plays an important role in tumor initiation progression. However, the epigenome-wide patterns portal vein thrombosis (PVTTs) have not been fully explored. Here, we performed DNA adjacent normal tissues (ANTs), paired PVTTs using Infinium HumanMethylation450 array (n = 11) conducted Sequenom EpiTYPER assays to confirm aberrantly methylated genes. MTS apoptosis assay were used assess synergistic effect two drugs on HCC cell...
To evaluate the safety of performing surgery on cavernous haemangiomas in liver larger than 10 cm and establish preoperative predictors intraoperative blood transfusion morbidity.A total 373 patients with who underwent our hospital were retrospectively analysed. According to tumour diameter, divided into a giant haemangioma (GH) group (241 cases) (10 ≤ diameter < 15 cm) an enormous (EH) (132 (diameter ≥ cm). Clinical parameters then compared between two groups.Compared GH group, EH had...
Abstract The liver is the most common site of metastasis in patients with colorectal cancer, and cancer (CRLM) associated poor rates survival. However, CRLM occurs infrequently livers exhibiting signs hepatitis or cirrhosis, suggesting a role for inflammation attenuating CRLM. molecular mechanisms driving this phenomenon remain unclear. aim study was to confirm mechanism by which inhibits We used BALB/c animal models inflammatory diseases that CRLM, then elucidated governing process. Out...