A5102819413- Genetic and Kidney Cyst Diseases
- Renal and related cancers
- Retinal Development and Disorders
- Virus-based gene therapy research
- Phytochemicals and Antioxidant Activities
- RNA Research and Splicing
- melanin and skin pigmentation
- Cancer Research and Treatments
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- Head and Neck Surgical Oncology
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Biochemical Analysis and Sensing Techniques
- Pregnancy and preeclampsia studies
- Urological Disorders and Treatments
- Retinoids in leukemia and cellular processes
- Systemic Lupus Erythematosus Research
- Sperm and Testicular Function
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- Sinusitis and nasal conditions
- RNA modifications and cancer
- interferon and immune responses
- MicroRNA in disease regulation
Beijing Tongren Hospital
2015-2025
Capital Medical University
2024-2025
Sichuan University
2025
West China Hospital of Sichuan University
2025
Shanghai Cell Therapy Research Institute
2025
Quanzhou Normal University
2025
Shanghai First People's Hospital
2025
Shanghai Jiao Tong University
2025
Fujian University of Traditional Chinese Medicine
2024
Shenyang Agricultural University
2024
There is a paucity of information about events that follow immediately after tumor cells are triggered to initiate the process angiogenesis (the formation new blood vessels). Such relevant issue when micrometastases vascularize and has implications for accessibility various treatments. In this study, we attempted monitor at initiation earliest possible stage growth in vivo.Two different rodent mammary cell lines, R3230Ac from Fischer 344 rat 4T1 BALB/c mouse, were stably transfected with...
In this study, the content, structure, antityrosinase activity, and mechanism of longan bark condensed tannins were evaluated. The findings obtained from mass spectrometry demonstrated that mixtures procyanidins, propelargonidins, prodelphinidins, their acyl derivatives (galloyl p-hydroxybenzoate). enzyme analysis indicated these efficient, reversible, mixed (competitive is dominant) inhibitor tyrosinase. What's more, showed good inhibitions on proliferation, intracellular activity...
Brain insulin signaling controls peripheral energy metabolism and plays a key role in the regulation of mood cognition. Epidemiological studies have indicated strong connection between type 2 diabetes (T2D) neurodegenerative disorders, especially Alzheimer’s disease (AD), linked via dysregulation signaling, i.e., resistance. While most focused on neurons, here, we aim to understand astrocytes, glial cell highly implicated AD pathology progression. To this end, created mouse model by crossing...
Abstract Type I IFN plays a key role in antiviral responses. It also has been shown that deregulation of type expression following abnormal activation TLRs contributes to the pathogenesis systemic lupus erythematosus. In this study, we find PIKfyve, class III lipid kinase, is required for endolysosomal TLR-induced mouse and human cells. PIKfyve binds phosphatidylinositol 3-phosphate synthesizes 3,5-bisphosphate, critical trafficking. However, modulates production via mechanisms independent...
To date, the non-viral vector Chimeric Antigen Receptor (CAR) T cell preparation platform, exemplified by transposons, has demonstrated significant potential in tumor immunotherapy and yielded positive results multiple clinical trials. Nonetheless, non-methylated CpG sequences within plasmid DNA can elicit an inflammatory response via Toll-like receptor 9 (TLR9) during CAR-T preparation, adversely affecting transgene expression. Additionally, de novo methylation programs promote exhaustion,...
Objective:To compare the clinical characteristics of nasal NK/T-cell lymphoma（NKTL） and diffuse large B-cell lymphoma（DLBCL） to improve diagnosis differential lymphomas. Methods:A retrospective analysis cases NKTL DLBCL was conducted. The symptoms, signs, imaging features both groups were compared statistically analyzed. Results: group showed more symptoms like exophthalmos/diplopia epiphora （both P=0.040）. likely be misdiagnosed as sinusitis（P=0.007）. In cases, mucosal swelling（P<0.01）,...
Smooth muscle myosin light chain kinase (smMLCK) is a Ca(2+)-calmodulin (CaM)-dependent enzyme that phosphorylates the 20-kDa chains of myosin. In previous study (Bagchi, I.C., Kemp, B.E., and Means, A.R. (1989) J. Biol. Chem. 264, 15843-15849), we expressed in bacteria 40-kDa fragment smMLCK displayed Ca(2+)-CaM-regulated catalytic activity. Initial mutagenesis experiments indicated Gly811 Arg812 were important for CaM-dependent activation this enzyme. We have now carried out site-directed...
Transcription factors are proteins that bind to DNA sequences regulate gene transcription. The transcription factor binding sites short (5–20 bp long) specifically bound by one or more factors. identification of and prediction their function continue be challenging problems in computational biology. In this study, integrating the DNase I hypersensitive with known position weight matrices TRANSFAC database, regulatory region identified. Based on global expression patterns cervical cancer...
Phosphorylation of regulatory light chain (MLC) activates myosin II, which enables it to promote contractile and motile activities cells. We report here a novel signaling mechanism that MLC phosphorylation smooth muscle contraction. Contractile agonists activated Rac1, Rac1 inhibition diminished agonist-induced phosphorylation, thus inhibiting inhibits the activity phosphatase (MLCP) but not kinase, through targets MYPT1 (a subunit MLCP) CPI-17 MLCP specific inhibitor) rather than RhoA-Rho...
Abstract Insulin and IGF-1 actions in vascular smooth muscle cells (VSMC) are associated with accelerated arterial intima hyperplasia restenosis after angioplasty, especially diabetes. To distinguish their relative roles, we delete insulin receptor (SMIRKO) or (SMIGF1RKO) VSMC mice. Here report that is attenuated SMIRKO mice, but not SMIGF1RKO In VSMC, deleting IGF1R increases homodimers of IR, enhances binding, stimulates p-Akt proliferation, IR decreases responses to IGF-1. Studies using...
Abstract Retinoblastoma is the most common intraocular malignancy in children with poor prognosis. The progression of retinoblastoma dependent on a robust angiogenic response. Targeting both cells and angiogenesis may therefore provide an alternative therapeutic strategy retinoblastoma. Here, we demonstrated inhibitory effects tigecycline, FDA-approved antibiotic, vitro vivo . We showed that tigecycline significantly inhibited growth induced caspase-dependent apoptosis multiple cell lines....
Abstract Intraflagellar transport protein 74 (IFT74) is a component of the core intraflagellar complex, bidirectional movement large particles along axoneme microtubules for cilia formation. In this study, we investigated its role in sperm flagella formation and discovered that mice deficiency Ift74 gene male germ cells were infertile with low count immotile sperm. The few developed spermatozoa displayed misshaped heads short tails. Transmission electron microscopy revealed abnormal...
Intraflagellar transport (IFT) is an evolutionarily conserved mechanism that indispensable for the formation and maintenance of cilia flagella; however, implications functions IFT81 remain unknown. In this study, we disrupted expression in male germ cells starting from spermatocyte stage. As a result, homozygous mutant males were completely infertile displayed abnormal sperm parameters. addition to oligozoospermia, spermatozoa presented dysmorphic nonfunctional flagella. Histological...
Abstract The dysregulation of YAP activity is implicated in abnormal organ size and the pathogenesis diverse diseases, including cancer. However, functional regulation by lncRNA-encoded peptides remains elusive. In this study, we report identification a small protein (93 aa) encoded lncRNA LINC01315. This protein, termed YAPer-ORF, preferentially interacted with GNAQ/11 mutants to augment activity. Mechanistically, YAPer-ORF was located nucleus competed bind nuclear kinase PRP4K hinder...
Background Chronic rhinosinusitis (CRS) without nasal polyps (CRSsNP) and with (CRSwNP) is reported to involve different inflammatory processes in sinonasal mucosa bone tissue, these remain uncharacterized. Objective We aimed investigate the molecular mechanisms of osteitis Chinese patients CRS better understand pathogenesis CRS. Methods The study included 10 controls, 16 CRSsNP, 23 CRSwNP. Ethmoid tissue samples were evaluated by histologic examination. Quantitative real-time reverse...