A5101434905- Genomics and Rare Diseases
- Genetic Associations and Epidemiology
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Optical Coherence Tomography Applications
- Medical Image Segmentation Techniques
- RNA Research and Splicing
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Microtubule and mitosis dynamics
- Coronary Interventions and Diagnostics
- Bacterial Identification and Susceptibility Testing
- Skin and Cellular Biology Research
- Carcinogens and Genotoxicity Assessment
- Medical Imaging Techniques and Applications
- Air Quality and Health Impacts
- Indoor Air Quality and Microbial Exposure
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- BRCA gene mutations in cancer
- Advanced MRI Techniques and Applications
- Reservoir Engineering and Simulation Methods
- Hair Growth and Disorders
- Infective Endocarditis Diagnosis and Management
Institute of Psychology, Chinese Academy of Sciences
2025
University of Chinese Academy of Sciences
2025
Shanghai Mental Health Center
2025
Shanghai Jiao Tong University
2025
Color (United States)
2019-2024
Cardiff University
2022
University of Bristol
2022
Centre Île-de-France - Versailles-Grignon
2022
Charles Sturt University
2022
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2022
ABSTRACT Sepsis is a major cause of morbidity, mortality, and increased medical expense. Rapid diagnosis improves outcomes reduces costs. The FilmArray blood culture identification panel (BioFire Diagnostics LLC, Salt Lake City, UT), highly multiplexed PCR assay, can identify 24 etiologic agents sepsis (8 Gram-positive, 11 Gram-negative, 5 yeast species) three antimicrobial resistance genes ( mecA , vanA / B bla KPC ) from positive bottles. It provides results in about 1 h with 2 min for...
<h3>Importance</h3> About 20% to 30% of people with schizophrenia have psychotic symptoms that do not respond adequately first-line antipsychotic treatment. This clinical presentation, chronic and highly disabling, is known as<i>treatment-resistant schizophrenia</i>(TRS). The causes treatment resistance their relationships underlying are largely unknown. Adequately powered genetic studies TRS scarce because the difficulty in collecting data from well-characterized cohorts. <h3>Objective</h3>...
The neural cell-specific N1 exon of the c-src pre-mRNA is both negatively regulated in nonneural cells and positively neurons. We previously identified conserved intronic elements flanking that direct repression splicing a HeLa cell extract. upstream repressor are located within polypyrimidine tract 3' splice site. A short RNA containing this site sequence can sequester trans-acting factors extract to allow N1. now show these specifically interact with binding protein (PTB). Mutations reduce...
The proteins and RNA regulatory elements that control tissue-specific pre-mRNA splicing in mammalian cells are mostly unknown. In this study, a set of is identified binds to element downstream the neuron specific c-src N1 exon. This complex bound specifically short containing sequence neuronal extracts splice It was not seen non-neuronal cell fail UV-cross-linking experiments neuron-specific 75-kD protein several nontissue-specific proteins, including 53-kD heterogeneous nuclear...
We describe results from a multicenter study evaluating the Accelerate Pheno system, first of its kind diagnostic system that rapidly identifies common bloodstream pathogens positive blood cultures within 90 min and determines bacterial phenotypic antimicrobial susceptibility testing (AST) ∼7 h. A combination fresh clinical seeded were tested, compared to Vitek 2 for identification (ID) broth microdilution or disk diffusion AST. The accurately identified 14 two Candida spp. with...
Recent advancements in next-generation sequencing have greatly expanded the use of multi-gene panel testing for hereditary cancer risk. Although genetic helps guide clinical diagnosis and management, recommendations are based on personal family history ethnicity, many carriers being missed. Herein, we report results from 23,179 individuals who were referred 30-gene risk, independent current guidelines-38.7% would not met National Comprehensive Cancer Network criteria testing. We identified a...
We introduce a method to determine the retinal nerve fiber layer (RNFL) thickness in OCT images based on anisotropic noise suppression and deformable splines. Spectral-Domain Optical Coherence Tomography (SDOCT) data was acquired at 29 kHz A-line rate with depth resolution of 2.6 mum range 1.6 mm. Areas 9.6x6.4 mm2 6.4x6.4 were approximately 6 seconds. The spline algorithm determined vitreous-RNFL RNFL-ganglion cell/inner plexiform boundary, respectively, changes reflectivity, resulting...
Abstract A simple modification of the Salmonella liquid incubation assay previously developed for detecting mutagens in urine was used to determine mutagenic activity airborne particulate matter. The consists adding ten times more bacteria (approximately 10 9 per tube) and five less metabolic enzymes compared plate incorporation method. mixture volume is approximately 0.2 ml, incubated 90 min before pouring it according standard protocol. modified procedure (micro preincubation or...
The production of haploid gametes from diploid germ cells requires two rounds meiotic chromosome segregation after one round replication. Accurate involves the remodeling each pair homologous chromosomes around site crossover into a highly condensed and ordered structure. We showed that condensin, protein complex needed for mitotic compaction, restructures during meiosis in Caenorhabditis elegans. In particular, condensin promotes both condensation recombination sister chromatids. Condensin...
"Conserved Intron Elements Repress Splicing of a Neuron-Specific c-src Exon In Vitro." Molecular and Cellular Biology, 17(5), p. 2970This article refers to:Schizosaccharomyces pombe skp1+ Encodes Protein Kinase Related to Mammalian Glycogen Synthase 3 Complements cdc14 Cytokinesis Mutant
We present a novel multi-resolution variational framework for vascular optical coherence elastography (OCE). This method exploits prior information about arterial wall biomechanics to produce robust estimates of tissue velocity and strain, reducing the sensitivity conventional tracking methods both noise- strain-induced signal decorrelation. The strain estimation performance this new estimator is demonstrated in simulated OCT image sequences benchtop scanning sample.
Epigenetic changes are crucial for the generation of immunological memory. Failure to generate or maintain these will result in poor memory responses. Similarly, augmenting stabilizing correct epigenetic states offers a potential method enhancing Yet transcription factors that regulate processes poorly defined. We find factor Oct1 and its cofactor OCA-B selectively required vivo CD4+ T cells. More importantly, cells formed do not respond properly antigen reencounter. In vitro, both proteins...
ABSTRACT Objective Anorexia nervosa (AN) is associated with disturbances in reward processing, cognitive control, and body image perception, implicating striatal dysfunction. Evidence suggests that underweight may modulate brain function AN. We aimed to investigate whole‐brain resting‐state functional connectivity (rsFC) of the striatum patients AN while controlling for acute effects underweight. Method Using theoretically selected sub‐regions, rsFC patterns were compared among ( n = 39, BMI...
Social cognition, the perception and processing of social information, is adversely affected in multiple psychiatric, neurological, neurodevelopmental disorders, these impairments negatively impact quality life for individuals across globe. Despite clear importance efforts to advance research via harmonization data cultures diagnoses has been stymied by lack uniformly used suitable assessments. To address this issue, current study conducted an expert survey consensus process identify...
The neuron-specific N1 exon of the mouse c-src transcript is normally skipped in nonneuronal cells. In this study, we examined sequence requirements for exclusion HeLa cell nuclear extracts. We found that repression mediated by specific intron sequences flank exon. Mutagenesis experiments identified conserved CUCUCU elements within these regions are required splicing. addition an RNA competitor containing upstream regulatory to extract induced splicing downstream N1, indicating binds...
Gene-specific and chromosome-wide mechanisms of transcriptional regulation control development in multicellular organisms. SDC-2, the determinant hermaphrodite fate Caenorhabditis elegans, is a paradigm for both modes regulation. SDC-2 represses transcription X chromosomes to achieve dosage compensation, it also male sex-determination gene her-1 elicit differentiation. We show here that recruits entire compensation complex her-1, directing this X-chromosome repression machinery silence an...
In meiosis, programmed DNA breaks repaired by homologous recombination (HR) can be processed into inter-homolog crossovers that promote the accurate segregation of chromosomes. general, more double-strand (DSBs) are formed than number crossovers, and excess DSBs must to maintain genomic stability. Sister-chromatid (inter-sister) is postulated important for completion meiotic DSB repair. However, this hypothesis difficult test because limited experimental means disrupt inter-sister not HR in...
To use whole genome sequencing to describe the likely origin of an outbreak Pseudomonas aeruginosa in a neonatal unit.Outbreak investigation.The intensive care unit service major obstetric tertiary referral center.Infants admitted who developed P. colonization or infection.We undertook strains isolated from colonized infants and environment.Eighteen were with aeruginosa. Isolates 12 7 environmental samples sequenced. All but one clinical isolates clustered ST253 no differences detected...
RBX1 (RING box protein 1), also known as ROC1 (Regulator of Cullin is an essential component SCF (Skp1/Cullins/F-box) E3 ubiquitin ligases, which target diverse proteins for proteasome-mediated degradation. Our recent study showed that silencing triggered a DNA damage response (DDR) leading to G(2)-M arrest, senescence, and apoptosis, with the mechanism remaining elusive. Here, we show that, in human cancer cells, causes accumulation replication licensing CDT1 ORC1, double-strand breaks,...
Since the introduction of 3-D rotational X-ray imaging, protocols for coronary artery imaging have become widely available in routine clinical practice. Intra-procedural cardiac a computed tomography (CT)-like fashion has been particularly compelling due to reduction overhead and ability characterize anatomy at time intervention. We previously introduced clinically feasible approach left atrium pulmonary veins (LAPVs) with short contrast bolus injections scan times approximately 4 -10 s. The...