A5051826848- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Autophagy in Disease and Therapy
- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Microtubule and mitosis dynamics
- Protease and Inhibitor Mechanisms
- NF-κB Signaling Pathways
- Cancer Research and Treatments
- Endoplasmic Reticulum Stress and Disease
- Histone Deacetylase Inhibitors Research
- interferon and immune responses
- Pancreatic function and diabetes
- Prostate Cancer Treatment and Research
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Cellular transport and secretion
- Hippo pathway signaling and YAP/TAZ
- DNA Repair Mechanisms
- Cancer, Lipids, and Metabolism
Zhejiang University
2016-2025
Ministry of Education of the People's Republic of China
2025
Zhejiang Lab
2024-2025
Second Affiliated Hospital of Zhejiang University
2018-2024
Binzhou University
2024
Zhejiang Center for Disease Control and Prevention
2024
Shanghai First People's Hospital
2024
Inner Mongolia University of Science and Technology
2024
Baotou Medical College
2024
Guangzhou Medical University
2021-2024
This assay for superoxide dismutase (SOD, EC 1.15.1.1) activity involves inhibition of nitroblue tetrazolium reduction, with xanthine-xanthine oxidase used as a generator. By using reaction terminator, we can determine 40 samples within 55 min. One unit pure bovine liver Cu,ZnSOD and chicken MnSOD was expressed by 30 ng 500 protein, respectively. The mean concentrations measured this method in blood from normal adults were 242 (SEM 4) mg/L erythrocytes, 548 20) micrograms/L serum, 173 11)...
We have designed MI-219 as a potent, highly selective and orally active small-molecule inhibitor of the MDM2–p53 interaction. binds to human MDM2 with K i value 5 nM is 10,000-fold for over MDMX. It disrupts interaction activates p53 pathway in cells wild-type p53, which leads induction cell cycle arrest all apoptosis tumor cells. stimulates rapid but transient activation established xenograft tissues, resulting inhibition proliferation, apoptosis, complete growth inhibition. normal tissues...
Abstract Posttranslational neddylation of cullins in the Cullin-Ring E3 ligase (CRL) complexes is needed for proteolytic degradation CRL substrates, whose accumulation induces cell-cycle arrest, apoptosis, and senescence. The Nedd8-activating enzyme (NAE) critical their growth-promoting function. Recently, anticancer small molecule MLN4924 currently phase I trials was determined to be an inhibitor NAE that blocks cullin inactivates CRL, triggering substrates trigger senescence cancer cells....
Microalgae, a naturally present unicellular microorganism, can undergo light photosynthesis and have been used in biofuels, nutrition, etc. Here, we report that engineered live microalgae be delivered to hypoxic tumor regions increase local oxygen levels resensitize resistant cancer cells both radio- phototherapies. We demonstrate the environment tumors is markedly improved by situ-generated through microalgae-mediated photosynthesis, resulting notably radiotherapeutic efficacy. Furthermore,...
Type 2 diabetes mellitus (T2DM) is recognized as a chronic, low-grade inflammatory disease characterized by insulin resistance and pancreatic β cell dysfunction; however, the underlying molecular mechanism remains unclear. Here, we report key cell-macrophage crosstalk pathway mediated miRNA-29-TNF-receptor-associated factor 3 (TRAF3) axis. cell-specific transgenic miR-29a/b/c mice are predisposed to develop glucose intolerance when fed high-fat diet (HFD). The metabolic effect of miR-29...
Abstract NEDD8 (Neural precursor cell expressed developmentally downregulated protein 8) is an ubiquitin-like that covalently attached to a lysine residue of substrate through process known as neddylation, catalyzed by the enzyme cascade, namely activating (E1), conjugating (E2), and ligase (E3). The substrates neddylation are categorized into cullins non-cullin proteins. Neddylation activates CRLs (cullin RING ligases), largest family E3 ligases, whereas alters their stability activity,...
Background —Extracellular matrix turnover is regulated by metalloproteinases (MMPs) and a family of tissue inhibitors (TIMPs). Together, these proteins may contribute to myocardial remodeling in congestive heart failure. We hypothesized that the expression MMPs TIMPs might be differentially failing human heart. Methods Results —Northern blot analyses were performed with probes TIMP-1 -4 GAPDH poly A + mRNA from ventricular tissues patients ischemic cardiomyopathy (ICM, n=16) or idiopathic...
p53, a tumor suppressor and transcription factor, has been shown to transcriptionally activate the expression of number important genes involved in regulation cell growth, DNA damage, angiogenesis, apoptosis. In computer search for other potential p53 target genes, we identified perfect binding site promoter human type IV collagenase (also called 72-kDa gelatinase or matrix metalloproteinase 2 [MMP-2]) gene. This was found specifically bind protein gel shift assay. Transcription assays with...
Glutathione peroxidase (GPX) is a primary antioxidant enzyme that scavenges hydrogen peroxide or organic hydroperoxides. We have recently found GPX induced by etoposide, topoisomerase II inhibitor and p53 activator. In search for <i>cis-</i>element confers potential regulation of GPX, we identified binding site in the promoter <i>GPX</i> gene. This bound to purified as well nuclear extract activated etoposide. A luciferase reporter driven 262-base pair fragment was transcriptionally wild...
Identification and characterization of p53 target genes would lead to a better understanding functions p53-mediated signaling pathways. Two putative binding sites were identified in the promoter gene encoding PTGF-beta, type beta transforming growth factor (TGF-beta) superfamily member. Gel shift assay showed that bound both sites. Luciferase-coupled transactivation revealed was activated dose- as well site-dependent manner by wild-type but not several mutants. The PTGF-beta enhanced...
Carcinogenesis is a multistage process, involving oncogene activation and tumor suppressor gene inactivation as well complex interactions between host tissues, leading ultimately to an aggressive metastatic phenotype. Among many genetic lesions, mutational of p53 suppressor, the "guardian genome," most frequent event found in 50% human cancers. plays critical role suppression mainly by inducing growth arrest, apoptosis, senescence, blocking angiogenesis. In addition, generally confers cancer...
Small-molecule Smac mimetics are being developed as a novel class of anticancer drugs. Recent studies have shown that target cellular inhibitor apoptosis protein (cIAP)-1/2 for degradation and induce tumor necrosis factor-alpha (TNFalpha)-dependent in cells. In this study, we investigated the mechanism action therapeutic potential two different types mimetics, monovalent SM-122 bivalent SM-164. Our data showed removal cIAP-1/2 by or small interfering RNA is not sufficient robust...
Skeletal muscle is the primary site of dietary glucose disposal, a function that depends on insulin-mediated exocytosis GLUT4 vesicles to its cell surface. In skeletal and adipocytes, this response involves Akt signaling Rab-GAP (GTPase-activating protein) AS160/TBC1D4. Intriguingly, AS160-targeted Rabs appear differ, with Rab8A participating in cells Rab10 their activation by insulin unknown. 8A, 10, 13 belong same subfamily Rab-GTPases. Here we show promotes GTP loading Rab13 but not rat...
A number of oncoproteins and tumor suppressors are known to be neddylated, but whether the neddylation pathway is entirely activated in human cancer remains unexplored.NEDD8-activating enzyme (NAE) (E1) NEDD8-conjugating (E2) expression global-protein were examined by immunohistochemistry, immunoblotting, real-time polymerase chain reaction analysis. Cell proliferation, clonogenic survival, migration, motility vitro, as well formation metastasis vivo, determined upon inhibition MLN4924, an...