A5022992165- Neurobiology and Insect Physiology Research
- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- Photochromic and Fluorescence Chemistry
- Environmental DNA in Biodiversity Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Histone Deacetylase Inhibitors Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect and Arachnid Ecology and Behavior
- Advanced Energy Technologies and Civil Engineering Innovations
- Cellular transport and secretion
- Renal and related cancers
- Sperm and Testicular Function
- Silk-based biomaterials and applications
- Genetics and Neurodevelopmental Disorders
- Animal Behavior and Reproduction
Johns Hopkins University
2022-2023
Johns Hopkins Medicine
2022-2023
Drexel University
2017-2019
John Wiley & Sons (United States)
2019
Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here, we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study. With over 44,000 nuclei 6000 cells analyzed, identification rare types,...
Adult stem cells are maintained in niches, specialized microenvironments that regulate their self-renewal and differentiation. In the adult Drosophila testis cell niche, somatic hub produce signals adjacent germline (GSCs) cyst (CySCs). Hub normally quiescent, but after complete genetic ablation of CySCs, they can proliferate transdifferentiate into new CySCs. Here we find Epidermal growth factor receptor (EGFR) signaling is upregulated CySC ability testes to recover from inhibited by...
Axon pruning is critical for sculpting precise neural circuits. Although axon has been described in the literature decades, relatively little known about molecular and cellular mechanisms that govern vivo. Here, we show epigenetic reader Kismet (Kis) required developmental Drosophila mushroom bodies. Kis binds to cis-regulatory elements of steroid hormone receptor ecdysone (ecr) gene necessary activating expression EcR-B1. promotes active H3K36 di- tri-methylation H4K16 acetylation histone...
Abstract Chromatin remodeling proteins of the chromodomain DNA-binding protein family, CHD7 and CHD8, mediate early neurodevelopmental events including neural migration differentiation. As such, mutations in either can lead to disorders. How chromatin influence activity mature synapses, however, is relatively unexplored. A critical feature neurons well-regulated endocytosis, which vital for synaptic function recycle membrane enabling continued release vesicles. Here we show that Kismet,...
Mannitol, a sugar alcohol used in commercial food products, has been previously shown to induce sex-biased mortality female Drosophila melanogaster when ingested at single concentration (1 M). We hypothesized that sex differences energy needs, related reproductive costs, contributed the increased we observed females compared males. To test this, longevity of actively mating and non-mating flies fed increasing concentrations mannitol. also asked whether mannitol-induced was...
Abstract Proper differentiation of sperm from germline stem cells, essential for production the next generation, requires dramatic changes in gene expression that drive remodeling almost all cellular components, chromatin to organelles cell shape itself. Here we provide a single nucleus and RNA-seq resource covering spermatogenesis Drosophila starting in-depth analysis adult testis (snRNA-seq) data Fly Cell Atlas (FCA) study (Li et al ., 2022). With over 44,000 nuclei 6,000 cells analyzed,...
Abstract The Class I basic helix–loop–helix (bHLH) proteins are highly conserved transcription factors that ubiquitously expressed. A wealth of literature on bHLH has shown these must homodimerize or heterodimerize with tissue‐specific HLH in order to bind DNA at E‐box consensus sequences control transcription. Due its ubiquitous expression, also extensively regulated posttranslationally, mostly through dimerization. Previously, we reported addition role promoting neurogenesis, the protein...
Summary Axon pruning is critical for the proper synapse elimination required sculpting precise neural circuits. Though axon has been described in literature decades, relatively little known about molecular and cellular mechanisms that govern vivo . Here, we show epigenetic reader Kismet (Kis) binds to cisregulatory elements of steroid hormone receptor Ecdysone Receptor ( EcR ) gene Drosophila neurons. Kis activate transcription at these promote H3K36 di- tri-methylation H4K16 acetylation. We...
Abstract The class I basic Helix Loop (bHLH) proteins are highly conserved transcription factors that ubiquitously expressed. A wealth of literature on bHLH have shown these must homodimerize or heterodimerize with tissue specific HLH in order to bind DNA at E box (CANNTG) consensus sequences control transcription. Due its ubiquitous expression, also extensively regulated post-translationally, mostly through dimerization. Previously, we reported addition role promoting neurogenesis, the...