A5101648265- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
- Cardiac Valve Diseases and Treatments
- Physiological and biochemical adaptations
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Immune responses and vaccinations
- Sphingolipid Metabolism and Signaling
- Child Nutrition and Water Access
- Parasites and Host Interactions
- Hippo pathway signaling and YAP/TAZ
- Cardiac and Coronary Surgery Techniques
- Pulmonary Hypertension Research and Treatments
- Autophagy in Disease and Therapy
- Cellular transport and secretion
- Cardiac Structural Anomalies and Repair
- Insect and Arachnid Ecology and Behavior
- Cardiac Ischemia and Reperfusion
- PI3K/AKT/mTOR signaling in cancer
- Cancer-related Molecular Pathways
- Congenital Heart Disease Studies
- Insect Utilization and Effects
- Wnt/β-catenin signaling in development and cancer
University of Utah
2017-2025
Huntsman Cancer Institute
2017-2024
Heidelberg University
2017-2022
German Cancer Research Center
2017-2022
E2F transcription factors are master regulators of the eukaryotic cell cycle. In Drosophila, sole activating E2F, E2F1, is both required for and sufficient to promote G1→S progression. E2F1 activity regulated by binding RB Family repressors posttranscriptional control protein levels EGFR TOR signaling pathways. Here, we investigate cis-regulatory elements in E2f1 messenger RNA (mRNA) that enable translation respond these signals mitotic proliferation wing imaginal disc intestinal stem cells....
Abstract JNK signaling is a critical regulator of inflammation and regeneration, but how it controlled in specific tissue contexts remains unclear. Here we show that, the Drosophila intestine, TNF-type ligand, Eiger (Egr), expressed exclusively by intestinal stem cells (ISCs) enteroblasts (EBs), where induced stress during aging. Egr preferentially activates paracrine fashion differentiated enterocytes (ECs) via its receptor, Grindelwald (Grnd). N -glycosylation genes ( Alg3 , Alg9 )...
Abstract We have previously identified that S. mansoni infection induces a unique form of myeloid training protects male but not female mice from high fat diet induced disease. Here we demonstrate ovarian derived hormones account for this sex specific difference. Ovariectomy females prior to permits metabolic reprogramming the lineage, with BMDM exhibiting carbon source flexibility cellular respiration, and protected systemic The innate phenotype can be replicated by in vivo injection SEA,...
The Hippo/Yki and RB/E2F pathways both regulate tissue growth by affecting cell proliferation survival, but interactions between these parallel control systems are poorly defined. In this study, we demonstrate that interaction Drosophila E2F1 Sd disrupts Yki/Sd complex formation thereby suppresses Yki target gene expression. RBF modifies effects reducing E2F1/Sd interaction. This regulation has significant on apoptosis, organ size, progenitor proliferation. Using a combination of DamID-seq...
In recent years, live-imaging techniques have been developed for the adult midgut of Drosophila melanogaster that allow temporal characterization key processes involved in stem cell and tissue homeostasis. However, these organ culture limited to imaging sessions < 16 hours, an interval too short track dynamic such as damage responses regeneration, which can unfold over several days. Therefore, we explant protocol capable sustaining midguts ex vivo up 3 This was made possible by...
ABSTRACT Adaptive changes in organ size and physiology occur most adult animals, but how these are regulated is not well understood. Previous research found that mating Drosophila females drives only increases gut stem cell proliferation also alters feeding behavior, intestinal gene expression, whole-body lipid storage, suggesting altered metabolism. Here, we show dramatically female metabolism digestive function. In addition to promoting a preference for high-protein diet, levels of TCA...
Cytosolic Ca2+ is a highly dynamic, tightly regulated and broadly conserved cellular signal. dynamics have been studied widely in monocultures, yet organs vivo comprise heterogeneous populations of stem differentiated cells. Here, we examine the adult Drosophila intestine, self-renewing epithelial organ which cells continuously produce daughters that differentiate into either enteroendocrine or enterocytes. Live imaging whole ex reveals stem-cell adopt strikingly distinct patterns...
Heart failure (HF) involves global genome modifications. Adverse structural and function remodeling that occurs in HF was initially considered to be irreversible. However, ~15% of patients on left ventricular assist device (LVAD) support show significant functional reverse remodeling, distinguishing them as responders (R), while ~85% are non-responders (NR). DNA methylation transcriptional changes exert myocardial recovery is not well understood. To address this, we performed whole-genome...
ABSTRACT Sphingolipids, including ceramides, are an important component of high-fat diets. These molecules can regulate fatty acid oxidation and intestinal stem cell proliferation, predisposing the gut to tumorigenesis. However, molecular mechanisms involved in ceramide metabolism-mediated (ISC) proliferation tumorigenesis poorly understood. To understand how changes sphingolipid metabolite flux affect cells, we manipulated activities each enzymes synthetic pathway using type-specific...
Abstract Injured epithelial organs must rapidly replace damaged cells to restore barrier integrity and physiological function. In response, injury-born stem cell progeny differentiate faster compared healthy-born counterparts, yet the mechanisms that pace differentia-tion are unclear. Using adult Drosophila intestine, we find injury speeds differentiation by altering lateral inhibition circuit transduces a fate-determin-ing Notch signal. During healthy intestinal turnover, balanced ratio of...
EGFR-RAS-ERK signaling promotes growth and proliferation in many cell types, genetic hyperactivation of RAS-ERK is a common driver cancers. Yet despite intensive study upstream components EGFR signal transduction, the identities functions downstream effectors pathway are largely unknown. Knowledge these effector mechanisms will provide novel routes for treatment diseases dysregulated growth, notably cancer. In Drosophila intestinal stem cells (ISC) transcriptional repressor Capicua (cic) its...
Abstract In recent years, live-imaging techniques have been developed for the adult midgut of Drosophila melanogaster that allow temporal characterization key processes involved in stem cell and tissue homeostasis. However, current organ culture are limited to imaging sessions ≤16 hours, an interval too short track dynamic such as damage responses regeneration, which can unfold over several days. Therefore, we a new explant protocol capable sustaining midguts ex vivo up 3 This was made...
ABSTRACT Cytosolic calcium is a highly dynamic, tightly regulated, and broadly conserved cellular signal. Calcium dynamics have been studied widely in monocultures, yet vivo most organs comprise heterogeneous populations of stem differentiated cells. We examined each cell type the adult Drosophila intestine, self-renewing epithelial organ where multipotent cells give rise to mature absorptive enterocytes secretory enteroendocrine Here we perform live imaging whole ex , employ orthogonal...