A5022583745- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Hippo pathway signaling and YAP/TAZ
- Epigenetics and DNA Methylation
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Cell Image Analysis Techniques
- Cancer-related Molecular Pathways
- Invertebrate Immune Response Mechanisms
- Developmental Biology and Gene Regulation
- Insect symbiosis and bacterial influences
- Viral Infectious Diseases and Gene Expression in Insects
- Genetics, Aging, and Longevity in Model Organisms
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Plant Molecular Biology Research
University of Helsinki
2017-2022
Heidelberg University
2011-2015
DKFZ-ZMBH Alliance
2011-2015
German Cancer Research Center
2011-2015
Fred Hutch Cancer Center
2011
North Seattle College
2011
University of Cologne
2006-2008
One promising approach for in vivo studies of cell proliferation is the FUCCI system (fluorescent ubiquitination-based cycle indicator).Here, we report development a Drosophila-specific (Fly-FUCCI) that allows one to distinguish G1, S, and G2 phases interphase.Fly-FUCCI relies on fluorochrome-tagged degrons from Cyclin B E2F1 proteins, which are degraded by ubiquitin E3-ligases APC/C CRL4 Cdt2 , during mitosis or onset S phase, respectively.These probes can track cell-cycle patterns cultured...
Endoreplicating cells undergo multiple rounds of DNA replication leading to polyploidy or polyteny. Oscillation Cyclin E (CycE)-dependent kinase activity is the main driving force in Drosophila endocycles. High levels CycE–Cdk2 trigger S phase, while down-regulation CycE-Cdk2 crucial allow licensing origins. In mitotic relicensing phase prevented by Geminin. Here we show that Geminin protein oscillates endoreplicating salivary glands . are high but drop once has been completed. coupled...
E2F transcription factors are master regulators of the eukaryotic cell cycle. In Drosophila, sole activating E2F, E2F1, is both required for and sufficient to promote G1→S progression. E2F1 activity regulated by binding RB Family repressors posttranscriptional control protein levels EGFR TOR signaling pathways. Here, we investigate cis-regulatory elements in E2f1 messenger RNA (mRNA) that enable translation respond these signals mitotic proliferation wing imaginal disc intestinal stem cells....
The transcription factor Myc drives cell growth across animal phyla and is activated in most forms of human cancer. However, it unclear which target genes need to be regulated induce whether multiple targets act additively or if induction each individually necessary. Here, we identified whose regulation conserved between humans flies deleted Myc-binding sites (E-boxes) the promoters fourteen these Drosophila. E-box mutants essential were homozygous viable, indicating that E-boxes are not...
Abstract The transcription factor Myc is activated in most major forms of human cancer. regulates a large set target genes, and drives cell growth across animal phyla. However, it has not been clear which genes are required for Myc-induced growth, whether the targets individually necessary or act an additive fashion. Here, we have used comparative functional genomics to identify core whose regulation conserved between humans Drosophila melanogaster . Most these essential involved ribosome...