A5070100494- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- Hippo pathway signaling and YAP/TAZ
- Advanced Fluorescence Microscopy Techniques
- Invertebrate Immune Response Mechanisms
- Force Microscopy Techniques and Applications
- Neonatal Respiratory Health Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Birth, Development, and Health
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Aquaculture disease management and microbiota
- Erythrocyte Function and Pathophysiology
- Plant Reproductive Biology
Philipps University of Marburg
2022-2024
Technische Universität Braunschweig
2017-2019
Zoological Institute
2018
Abstract Changes in cell morphology require the dynamic remodeling of actin cytoskeleton. Calcium fluxes have been suggested as an important signal to rapidly relay information cytoskeleton, but underlying mechanisms remain poorly understood. Here, we identify EF-hand domain containing protein EFhD2/Swip-1 a conserved lamellipodial strongly upregulated Drosophila macrophages at onset metamorphosis when macrophage behavior shifts from quiescent migratory state. Loss- and gain-of-function...
Cell motility is crucial for many biological processes including morphogenesis, wound healing, and cancer invasion. The WAVE regulatory complex (WRC) a central Arp2/3 regulator driving cell downstream of activation by Rac GTPase. CYFIP-related Rac1 interactor (CYRI) proteins are thought to compete with WRC interaction in feedback loop regulating lamellipodia dynamics. However, the physiological role CYRI vivo healthy tissues unclear. Here, we used Drosophila as model system study function at...
Sulfite is a neurotoxin, which detoxified by the molybdenum cofactor (Moco)-dependent enzyme sulfite oxidase (SOX). In humans, SOX deficiency causes formation of glutamate analog S-Sulfocysteine (SSC) resulting in constant overstimulation ionotropic glutamatergic receptors. Overstimulation leads to seizures, severe brain damage, and early childhood death. may be caused either mutated sox gene or mutations one genes multi-step Moco biosynthesis pathway. While patients affected first step can...
Exocytosis is a fundamental cellular process by which cells secrete cargos from their apical membrane into the extracellular lumen. Cargo release proceeds in sequential steps that depend on coordinated assembly and organization of an actin cytoskeletal network. Here, we identified conserved actin-crosslinking protein Swip-1 as novel regulator controlling exocytosis glue granules Drosophila salivary gland. Real-time imaging revealed simultaneously recruited with F-actin onto secreting...
Zebrafish embryos are transparent and develop rapidly outside the mother, thus allowing for excellent in vivo imaging of dynamic biological processes an intact developing vertebrate. However, detailed morphologies distinct cell types subcellular structures is limited whole mounts. Therefore, we established efficient easy-to-use protocol to culture live primary cells from zebrafish adult tissue. In brief, 2 dpf dechorionated, deyolked, sterilized, dissociated single with collagenase. After a...
ABSTRACT First Person is a series of interviews with the first authors selection papers published in Journal Cell Science, helping researchers promote themselves alongside their papers. Franziska Lehne author on ‘ Swip-1 promotes exocytosis glue granules exocrine Drosophila salivary gland’, JCS. conducted research described this article while PhD student Prof. Dr Sven Bogdan's lab at Institute Physiology and Pathophysiology, Department Molecular Physiology, Philipps-University Marburg,...