A5055924807- Advanced Fluorescence Microscopy Techniques
- Diverse Scientific and Economic Studies
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Neural dynamics and brain function
- Optical Coherence Tomography Applications
- Memory and Neural Mechanisms
- Photovoltaic System Optimization Techniques
- Electric Power System Optimization
- Neuroscience and Neuropharmacology Research
- Advanced Biosensing Techniques and Applications
- Advanced Memory and Neural Computing
University of California, Los Angeles
2023-2024
Recording the neural activity of biological organisms is paramount in understanding how they process world around them. Fluorescence microscopy has served as standard recording this due to its ability capture large populations neurons simultaneously. Recent efforts fluorescence have been concentrated imaging scale volumes, however, most these limited by spatiotemporal and bandwidth constraints. We present a novel system called Transverse-Sheet Illumination Microscopy (TranSIM), which...
Here we describe the development of UCLA 2P Miniscope, an easily adopted, open-source miniature 2-photon microscope capable recording calcium dynamics from neurons located in deep structures and dendrites over a 445 μm x 380 field view (FOV) during free behavior. The system weighs approximately 4g utilizes two on-board silicon-based photon detectors for highly sensitive measurements. All hardware is designed high performance ease assembly, while minimizing cost. To test microscope, recorded...
Abstract Neuronal spiking activity is routinely recorded using genetically encoded calcium indicators (GECIs), but imaging limited in temporal resolution and does not report subthreshold voltage changes. Genetically (GEVIs) offer better sensitivity, spike detection with fast GEVIs has required specialized equipment. Here, we the ASAP4 subfamily of that brighten response to membrane depolarization, inverting fluorescence-voltage relationship previous ASAP GEVIs. Two variants, ASAP4b ASAP4e,...
Hippocampal spiking sequences encode and link behavioral information across time. How inhibition sculpts these remains unknown. We performed longitudinal voltage imaging of CA1 parvalbumin- somatostatin-expressing interneurons in mice during an odor-cued working memory task, before after training. During this pyramidal odor-specific the cue throughout a delay period. In contrast, most encoded odor delivery, but not identity, nor Population was stable days, with constant field turnover,...