A5030710171- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
- Genetics and Neurodevelopmental Disorders
- Advanced Fluorescence Microscopy Techniques
- Receptor Mechanisms and Signaling
- Advanced Memory and Neural Computing
- Video Surveillance and Tracking Methods
- Advanced biosensing and bioanalysis techniques
- Neuropeptides and Animal Physiology
- Environmental Impact and Sustainability
- Advanced Image Fusion Techniques
- Forest Biomass Utilization and Management
- Apelin-related biomedical research
- Epilepsy research and treatment
- Image Enhancement Techniques
- DNA and Nucleic Acid Chemistry
- Bioenergy crop production and management
- Ubiquitin and proteasome pathways
- Neurogenesis and neuroplasticity mechanisms
- RNA modifications and cancer
- Photoreceptor and optogenetics research
- Neuroendocrine regulation and behavior
- Electrochemical Analysis and Applications
Stanford University
2019-2024
Stanford Medicine
2021-2023
Guilin University
2022
Guilin University of Electronic Technology
2019-2022
Pennsylvania State University
2013-2020
Parvalbumin (PV)-positive interneurons in the hippocampus play a critical role animal memory, such as spatial working memory. However, how PV-positive subregions of affect behaviors remains poorly defined. Here, we achieved specific and reversible activation using designer receptors exclusively activated by drugs (DREADD) technology. Inducible DREADD expression was demonstrated vitro cultured neurons, which co-transfection hM3D-Gq-mCherry vector with Cre plasmid resulted cellular response to...
Neuroplasticity depends on the precise timing of gene expression, which requires accurate control mRNA stability and rapid elimination abnormal mRNA. Nonsense-mediated decay (NMD) is an RNA surveillance mechanism that ensures speedy degradation mRNAs carrying premature termination codons (PTCs). This relies several key Exon Junction Complex (EJC) factors to distinguish PTCs from normal stop codons. NMD degrades not only aberrant transcripts PTCs, but also harboring a codon [1]. Intriguingly,...
Schizophrenia (SCZ) is a severe mental disorder characterized by delusion, hallucination, and cognitive deficits. We have previously identified from schizophrenia patients loss-of-function mutation Arg215→His215 (R215H) of neuroligin 2 (NLGN2) gene, which encodes cell adhesion molecule critical for GABAergic synapse formation function. Here, we generated novel transgenic mouse line with neuroligin-2 (NL2) R215H mutation. The single point caused significant loss NL2 protein in vivo, reduced...
The GABAA receptors are the major inhibitory in brain and localized at both synaptic extrasynaptic membranes. Synaptic mediate phasic inhibition, whereas tonic inhibition. Both inhibitions regulate neuronal activity, but whether they each other is not very clear. Here, we investigated functional interaction between through various molecular manipulations. Overexpression of α6β3δ-GABAA mouse hippocampal pyramidal neurons significantly increased currents. Surprisingly, increase inhibition was...
Abstract Background GABAergic deficit is one of the major mechanisms underlying epileptic seizures. Previous studies have mainly focused on alterations synaptic inhibition during epileptogenesis. Recent work suggested that tonic may also play a role in regulating epileptogenesis, but mechanism not well understood. Results We employed molecular and pharmacological tools to investigate epileptogenesis both vitro vivo . overexpressed two distinct subtypes extrasynaptic GABA A receptors, α5β3γ2...
Abstract Neuronal spiking activity is routinely recorded using genetically encoded calcium indicators (GECIs), but imaging limited in temporal resolution and does not report subthreshold voltage changes. Genetically (GEVIs) offer better sensitivity, spike detection with fast GEVIs has required specialized equipment. Here, we the ASAP4 subfamily of that brighten response to membrane depolarization, inverting fluorescence-voltage relationship previous ASAP GEVIs. Two variants, ASAP4b ASAP4e,...
ABSTRACT The recent positively tuned ASAP4-family voltage indicators feature superior photostability compared to the negatively ASAP3, but signal-to-noise ratios (SNRs) for spike detection were not significantly different. To improve by ASAP indicators, we performed multiple rounds of structure-guided saturation mutagenesis an ASAP4 template while screening directly faster responses. Our resulting variants, ASAP6.1 and ASAP6b, demonstrated sufficient dynamic range kinetics, reported action...
Significance Fragile X syndrome (FXS) is caused by loss of expression fragile mental retardation protein (FMRP), an RNA-binding that has been proposed to regulate local stimulus-dependent synthesis. However, how FMRP affects distributions newly synthesized proteins remains unclear. Here, we report BDNF application promotes persistent accumulation new PSD95 at stimulated synapses and dendrites, this absent in FMRP-deficient mouse neurons. We further identify signaling pathways important for...
Abstract Schizophrenia (SCZ) is a severe mental disorder characterized by delusion, hallucination, and cognitive deficits. We have previously identified from schizophrenia patients loss-of-function mutation Arg 215 → His (R215H) of neuroligin 2 (NLGN2) gene, which encodes cell adhesion molecule critical for GABAergic synapse formation function. Here, we generated novel transgenic mouse line with neuroligin-2 (NL2) R215H mutation, showed significant loss NL2 protein, reduced transmission,...
Aiming at the problems of low-illuminance image brightness, low contrast, and lack detailed information, a multi-scale weighted enhancement algorithm based on HSV space is proposed. The first converts from RGB to space; then uses information that can characterize characteristics in H S channels as weights enhance V channel; finally, guided filtering used details obtain strong Images with sharp edges details. To verify superiority algorithm, comparative experiment was carried out, objectively...
In this study, an algorithm for analysing the production of regional waste biomass was developed in Qixing District, Guilin, China. Population heat map, residential population, POI (Point interest) and GIS (Geographic Information System) data were collected from web. The MSW (Municipal solid waste) volume scope six transfer stations District surveyed to analyse computing weights each area potential biomass. An extrapolable visualization composition established. verified based on spot data....