A5045324134- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
- Bone health and osteoporosis research
- RNA Research and Splicing
- Nerve injury and regeneration
- Bone health and treatments
- Nuclear Structure and Function
- Retinal Development and Disorders
- Traumatic Ocular and Foreign Body Injuries
- Neurogenesis and neuroplasticity mechanisms
- RNA regulation and disease
- Ion channel regulation and function
- Shoulder and Clavicle Injuries
- Cellular Mechanics and Interactions
- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Spaceflight effects on biology
- Hand Gesture Recognition Systems
- Automotive and Human Injury Biomechanics
- Neuropeptides and Animal Physiology
- Trauma Management and Diagnosis
- RNA Interference and Gene Delivery
- Hemophilia Treatment and Research
- Metal and Thin Film Mechanics
- Bone fractures and treatments
Vancouver General Hospital
2024
College of New Jersey
2018-2024
Stanford University
2012-2022
University of North Carolina at Chapel Hill
2014-2016
University of Virginia
2006-2015
UNC/NCSU Joint Department of Biomedical Engineering
2014
Institute of Molecular and Cell Biology
2013
Institute of Behavioral Sciences
2013
University of Auckland
2009-2012
Emory University
2000-2010
Presynaptic Ca V 2.2 (N-type) calcium channels are subject to modulation by interaction with syntaxin 1 and a 1-sensitive Gα O G-protein pathway. We used biochemical analysis of neuronal tissue lysates new quantitative test colocalization intensity correlation at the giant calyx-type presynaptic terminal chick ciliary ganglion explore association . could be localized immunocytochemistry (antibody Ab571) in puncta on release site aspect close synaptic vesicle clouds. Syntaxin...
Expression of the brain-derived neurotrophic factor (BDNF) is under tight regulation to accommodate its intricate roles in controlling brain function. Transcription BDNF initiates from multiple promoters response distinct stimulation cues. However, regardless which promoter used, all transcripts are processed at two alternative polyadenylation sites, generating pools mRNAs that carry either a long or short 3'UTR, both encoding same protein. Whether and how 3'UTRs may differentially regulate...
The β1-adrenergic receptor (β1AR) is known to be localized synapses and modulate synaptic plasticity in many brain regions, but the molecular mechanisms determining β1AR subcellular localization are not fully understood. Using overlay pull-down techniques, we found that carboxyl terminus associates with MAGI-2 (membrane-associated guanylate kinase inverted-2), a protein also as S-SCAM (synaptic scaffolding molecule). multidomain contains nine potential protein-protein interaction modules,...
Members of the transmembrane AMPA receptor-regulatory protein (TARP) family modulate receptor (AMPA-R) trafficking and function. AMPA-Rs consist four pore-forming subunits. Previous studies show that TARPs are an integral part AMPA-R complex, acting as accessory subunits for mature receptors in vivo. The TARP/AMPA-R stoichiometry was previously measured indirectly found to be variable dependent on TARP expression level, with at most associated each complex. Here, we use a single-molecule...
β- and α2–adrenergic receptors are known to exhibit substantial cross-talk mutual regulation in tissues where they expressed together. We have found that the β1-adrenergic receptor (β1AR) α2A-adrenergic (α2AAR) heterodimerize when coexpressed cells. Immunoprecipitation studies with differentially tagged β1AR α2AAR HEK-293 cells revealed robust co-immunoprecipitation of two receptors. Moreover, agonist stimulation was induce internalization β1AR, providing further evidence for a physical...
PDZ domains bind to the carboxyl-termini of target proteins, and some are capable oligomerization facilitate formation intracellular signaling complexes. The Na+/H+ exchanger regulatory factor (NHERF-1; also called "EBP50") its relative NHERF-2 (also "E3KARP", "SIP-1", "TKA-1") both have two domains. We report here that NHERF-1 specifically each other but not Purified associate with robustly in absence any associated purified only weakly when examined alone. is greatly facilitated they bound...
Objective Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that elevated in the serum and synovial fluid of patients with osteoarthritis (OA). This study was undertaken to investigate potential role MIF OA human joint tissues vivo mice age‐related surgically induced OA. Methods conditioned media from chondrocytes meniscal cells cartilage explants measured by enzyme‐linked immunosorbent assay. The severity analyzed histologically male wild‐type −/− at 12 22 months...
Na+/H+exchanger regulatory factor (NHERF)-1 is a PDZ domain-containing adaptor protein known to bind various receptors, channels, cytoskeletal elements, and cytoplasmic signaling proteins. We report here that the phosphorylation state of NHERF-1 profoundly regulated by cell cycle: in HeLa cells hyperphosphorylated mitosis phase much less phosphorylated at other points cycle. This phase-dependent could be blocked roscovitine, consistent with cyclin-dependent kinases. In vitro studies purified...
Summary While chronic degenerative arthropathy is the main morbidity of haemophilia, a very high prevalence low bone density also seen in men and boys with haemophilia. This study investigates degradation knee joint haemophilic mice resulting from haemarthrosis efficacy aggressive treatment factor VIII period surrounding injury to prevent pathology. Skeletally mature knock‐out were subjected haemorrhage induced by puncture left capsule. Mice received either intravenous or placebo immediately...
Homeostatic synaptic plasticity is a non-Hebbian mechanism that adjusts strength to maintain network stability while achieving optimal information processing. Among the molecular mediators shown regulate this form of plasticity, signaling through retinoic acid (RA) and its receptor, RARα, has been be critically involved in homeostatic adjustment transmission both hippocampus sensory cortices. In study, we explore which postsynaptic RA RARα regulates presynaptic neurotransmitter release...
To improve understanding of structural coupling and deformation patterns throughout the loaded ribcage, present study reports force-displacement kinematic responses under a highly-localized loading condition using three PMHS ribcages (ages 44, 61, 63 years). The were quasi-statically locally to non-failure displacement (nominally 15% ribcage depth at rib level) approximately 25 unilateral locations 5-7 geometrically symmetric bilateral on anterior surface each for total 94 tests....
Objective: The goal of the present study was to identify rib-level differences in fracture characteristics for individual ribs subjected anterior–posterior loading. Methods: Twenty-seven were extracted from levels 2 10 3 postmortem human subjects (2 females and one male) loading at a quasistatic mm/s) rate. placed fixture that provided pinned boundary condition each extremity, specimen loaded failure. Reaction force strains on internal external cortical surfaces measured. Results: Rib found...
Abstract The glutamate receptor 2 (GluR2) subunit determines many of the functional properties α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionate subtype receptor. roles untranslated regions (UTRs) in mRNA stability, transport, or translation are increasingly recognized. 3′ end GluR2 transcripts alternatively processed to form a short and long 3′UTR, giving rise two pools 4 6 kb length, respectively, mammalian brain. However, role these alternative 3′UTRs expression has not been reported. We...
The literature is lacking the description of a device and method for simulating measuring shoe–turf interactions at loads rates generated in situ by elite athletes during performance. A transportable was built to quantify these through three tests that reflect generic classes tasks: 1) translation test; 2) rotation 3) translation/drop test. All were performed using cleated portion molded American football shoe on two types natural grass surfaces. To assess repeatability tests, we multiple...