A5013964212- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Neuroscience and Neuropharmacology Research
- Epigenetics and DNA Methylation
- Plant responses to water stress
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- RNA Research and Splicing
- Plant Gene Expression Analysis
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- Plant nutrient uptake and metabolism
- Immune Cell Function and Interaction
- Extracellular vesicles in disease
- Bone Metabolism and Diseases
- Postharvest Quality and Shelf Life Management
- Hibiscus Plant Research Studies
- Electromagnetic Fields and Biological Effects
- Allergic Rhinitis and Sensitization
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Nerve injury and regeneration
- Lipid Membrane Structure and Behavior
Sun Yat-sen University
2020-2025
Tufts Medical Center
2023-2024
Guangxi University
2016-2023
Université Paris Sciences et Lettres
2018-2023
Centre National de la Recherche Scientifique
2018-2023
Heilongjiang Bayi Agricultural University
2021-2023
Guangdong Medical College
2023
Zhanjiang Experimental Station
2023
Sorbonne Université
2021-2023
Dynamique de l'information génétique : bases fondamentales et cancer
2021-2023
Non-invasive methods of detecting cancer by circulating exosomes are challenged inefficient purification and identification. This study hereby proposed an automated centrifugal microfluidic disc system combined with functionalized membranes (Exo-CMDS) to isolate enrich exosomes, which will then be processed a novel aptamer fluorescence (Exo-AFS) in order detect the exosome surface proteins effective manner. Exo-CMDS features highly qualified yields optimal exosomal concentration 5.1 × 109...
Abstract Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map of single DNA molecules at 200-nucleotide resolution. By quantifying BrdU incorporation along pulse-chased intermediates from Saccharomyces cerevisiae , orient 58,651 tracks reproducing population-based directionality profiles and 4964 4485 individual initiation termination events, respectively. Although most events cluster...
Abstract Background Patients with peripheral nerve injury (PNI) often suffer from hypoxic ischemic impairments, in particular when combined vascular damage, causing neuronal dysfunction and death. Increasing attention has been paid on skin precursor-derived Schwann cells (SKP-SCs), previous study shown that SKP-SCs could promote sensory recovery after cell therapy for PNI, resembling the effect of naive SCs, SKP-SC-derived extracellular vesicles (SKP-SC-EVs) are putatively supposed to be...
Osteoporosis (OP) is one of the most common bone diseases, especially in women after menopause. Increasing evidence shows that non-coding RNAs are implicated pathogenesis OP. In this study, based on published circular RNA profiling data between OP patients and healthy controls, we found circRNA_0001795 (circ_0001795) downregulated samples, which was further validated samples collected study. We therefore investigated functional role molecular mechanism circ_0001795 osteogenic differentiation...
Brain structure and function experience dramatic changes from embryonic to postnatal development. Microarray analyses have detected differential gene expression at different stages in disease models, but information during early brain development is limited. We generated >27 million reads identify mRNAs the mouse cortex for >16,000 genes either day 18 (E18) or 7 (P7), a period of significant synaptogenesis neural circuit formation. In addition, we devised strategies detect alternative...
GABA(A) receptors (GABA(A)-Rs) are localized at both synaptic and extrasynaptic sites, mediating phasic tonic inhibition, respectively. Previous studies suggest an important role of γ2 δ subunits in versus targeting GABA(A)-Rs. Here, we demonstrate differential function α2 α6 guiding the localization To study specific subtypes GABA(A)-Rs, used a molecularly engineered GABAergic synapse model to precisely control GABA(A)-R subunit composition. We found that neuron-HEK cell heterosynapses,...
Eukaryotic DNA replication initiates during S phase from origins that have been licensed in the preceding G1 phase. Here, we compare ChIP-seq profiles of licensing factors Orc2, Orc3, Mcm3, and Mcm7 with gene expression, timing, fork directionality obtained by RNA-seq, Repli-seq, OK-seq. Both, origin recognition complex (ORC) minichromosome maintenance (MCM) are significantly homogeneously depleted transcribed genes, enriched at promoters, more abundant early- than late-replicating domains....
Abstract During each cell division, tens of thousands DNA replication origins are co-ordinately activated to ensure the complete duplication human genome. However, fork progression can be challenged by many factors, including co-directional and head-on transcription-replication conflicts (TRC). Head-on TRCs more dangerous for genome integrity. To study direction movement TRCs, we developed a bioinformatics toolkit called OKseqHMM (https://github.com/CL-CHEN-Lab/OK-Seq,...
Chromatin topology can impact gene regulation, but how evolutionary divergence in chromatin has shaped regulatory landscapes for distinctive human traits remains poorly understood. CTCF sites determine by forming domains and loops. Here, we show CTCF-mediated at the domain loop scales during primate evolution, elucidating distinct mechanisms shaping landscapes. Human-specific divergent lead to a broad rewiring of transcriptional Divergent loops concord with species-specific enhancer...
The spatiotemporal program of metazoan DNA replication is regulated during development and altered in cancers. We have generated novel OK-seq, Repli-seq RNA-seq data to compare the gene expression programs twelve cancer non-cancer human cell types. Changes fork directionality (RFD) determined by OK-seq are widespread but more frequent within GC-poor isochores largely disconnected from transcription changes. Cancer RFD profiles cluster with cells similar developmental origin not different...
Abstract Mitochondrial quality control is a new target for myocardial protection. Notch signaling plays an important role in heart development, maturation, and repair. However, the of mitochondrial remains elusive. In this study, we isolated cells from rats established ischemia reperfusion injury (IRI) model. We modulated Notch1 expression level via infection with recombinant adenoviruses Ad‐N1ICD Ad‐shN1ICD. found that IR reduced viability, but overexpression increased viability exposed to...
Abstract Mammalian genomes are replicated in a cell type-specific order and coordination with transcription chromatin organization. Currently, single-cell replication studies require individual processing of sorted cells, yielding limited number (<100) cells. Here, we develop Kronos scRT, software for Replication Timing (scRT) analysis. scRT does not specific platform or sorting, which allows investigating large datasets obtained from asynchronous By applying our tool to published data as...
Skin precursor-derived Schwann cells (SKP-SCs) have been shown to benefit the recovery of spinal cord injury (SCI) and peripheral nerve (PNI) with motor dysfunction. However, effect extracellular vesicles (EVs) from SKP-SCs responsible for neuroregeneration remains unknown.Based on obtainment identification rat their derived EVs, primary model motoneurons resulting axotomy in vitro or crush vivo, as well secondary ischemic hypoxic motoneuron exposure oxygen-glucose-deprivation (OGD) vitro,...
Alopecia affects millions of individuals globally, with hair loss becoming more common among young people. Various traditional Chinese medicines (TCM) have been used clinically for treating alopecia, however, the effective compounds and underlying mechanism are less known. We sought to investigate effect Alpinetin (AP), a compound extracted from Fabaceae Zingiberaceae herbs, in regeneration.Animal model regeneration was mimicked by depilation C57BL/6J mice. The mice were then topically...
Abstract The smooth muscle of the uterus during pregnancy presents a unique circumstance physiological mechanotransduction as tissue remodels in response to stretches imposed by growing foetus(es), yet nature molecular and functional adaptations remain unresolved. We studied, myometrium isolated from non‐pregnant (NP) pregnant mice, active passive length–tension curves myography expression activation immunoblotting focal adhesion‐related proteins known other systems participate...
Both the Notch1 and Keap1-Nrf2 signaling pathways have cardioprotective effects, but role of Notch1-Nrf2 crosstalk in myocardial ischemia-reperfusion injury is unclear. In this study, we established hypoxia-reoxygenation neonate rat cells employed γ-secretase inhibitor curcumin to inhibit activate pathways, respectively. We found that combined action significantly increased cardiomyocyte viability, inhibited apoptosis, reduced formation reactive oxygen species, antioxidant activities....
Type 2 diabetes mellitus (DM)-induced cardiomyopathy is a multifactorial and complex disease involving oxidative stress, lipids, fibrosis. It based on metabolic disorders microvascular causes extensive focal necrosis of the heart muscle. Curcumin (CUR) natural polyphenol isolated from turmeric rhizomes plays an important role in antioxidant, anti-apoptotic anti-inflammatory effects diabetes. Therefore, we established mouse model diabetic (DCM) type db/db mice our study. We divided experiment...