A5034487002- Cellular Mechanics and Interactions
- Digital Holography and Microscopy
- 3D Printing in Biomedical Research
- Pancreatic and Hepatic Oncology Research
- Cell Image Analysis Techniques
- Gut microbiota and health
- Chronic Myeloid Leukemia Treatments
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Blood properties and coagulation
- Chronic Lymphocytic Leukemia Research
- Erythrocyte Function and Pathophysiology
- Skin and Cellular Biology Research
- DNA Repair Mechanisms
- Force Microscopy Techniques and Applications
- Acute Myeloid Leukemia Research
- Advanced Fluorescence Microscopy Techniques
- Optical measurement and interference techniques
- Clostridium difficile and Clostridium perfringens research
- Microfluidic and Bio-sensing Technologies
- HER2/EGFR in Cancer Research
- Image Processing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Planarian Biology and Electrostimulation
Erytech Pharma (France)
2021-2025
Centre de Recherche en Cancérologie de Lyon
2012-2020
Centre National de la Recherche Scientifique
2012-2020
Canadian Nautical Research Society
2020
Lyon College
2020
Inserm
2012-2018
Université Claude Bernard Lyon 1
2012-2018
École Normale Supérieure de Lyon
2015-2018
Hôpital Lyon Sud
2018
Centre Léon Bérard
2018
The spatiotemporal program of metazoan DNA replication is regulated during development and altered in cancers. We have generated novel OK-seq, Repli-seq RNA-seq data to compare the gene expression programs twelve cancer non-cancer human cell types. Changes fork directionality (RFD) determined by OK-seq are widespread but more frequent within GC-poor isochores largely disconnected from transcription changes. Cancer RFD profiles cluster with cells similar developmental origin not different...
Abstract Background: Increasing evidence suggests that gut microbiome composition modulates tumor response to therapies, including immune checkpoint inhibitors (ICI). Clinical proofs of concept were obtained in pilot studies using healthy or ICI-responder donor-derived fecal matter transplants modulate the non-responding cancer patients and improve their ICI. We have developed Microbiome Ecosystem TherapiesTM (MET) replicating at large industrial scale effects ICI-responders microbiome....
Organoid cultures in 3D matrices are relevant models to mimic the complex vivo environment that supports cell physiological and pathological behaviors. For instance, epithelial organoids recapitulate numerous features of glandular tissues including development fully differentiated acini maintain apico-basal polarity with hollow lumen. Effective genetic engineering would bring new insights organogenesis carcinogenesis. However, direct transfection on already formed remains challenging. One...
We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multiangle acquisitions 3D cell cultures embedded in extracellular matrices. Lens-free present some inherent issues such as the lack of phase information sensor plane and limited angular coverage. developed compared three different algorithms Fourier diffraction theorem obtain fully reconstructions. These an increasing complexity associated with better reconstruction quality. Two them are...
The distribution of refractive indices (RIs) a living cell contributes in nonintuitive manner to its optical phase image and quite rarely can be inverted recover internal structure. interpretation the quantitative images cells remains difficult task because (1) we still have very little knowledge on impact macromolecular complexes local RI (2) changes produced by light propagation through sample are mixed with diffraction effects bodies. We propose implement two-dimensional wavelet-based...
Abstract Thanks to a novel three-dimensional imaging platform based on lens-free microscopy, it is possible perform multi-angle acquisitions and holographic reconstructions of 3D cell cultures directly into the incubator. Being able reconstructing volumes as large ~5 mm 3 over period time covering several days, allows us observe broad range migration strategies only present in environment, whether single migration, collective migrations cells dispersal cells. In addition we are distinguish...
Growing evidence demonstrates the key role of gut microbiota in human health and disease. The recent success microbiotherapy products to treat recurrent
A unique tool to analyze the role of long-term effects mechanical confinement in normal and pathological conditions.
Cancer cell transformation is often accompanied by a modification of their viscoelastic properties. When capturing the stress-to-strain response primary chronic myelogenous leukemia (CML) cells, from two data sets CD34+ hematopoietic cells isolated healthy and leukemic bone marrows, we show that mean shear relaxation modulus increases upon cancer transformation. This stiffening comes along with local rupture events, detected as reinforced sharp maxima this modulus, suggesting these respond...
Red blood cells (RBCs) can act as carriers for therapeutic agents and substantially improve the safety, pharmacokinetics, pharmacodynamics of many drugs. Maintaining RBCs integrity lifespan is important efficacy drug carrier. We investigated impact encapsulation by hypotonic dialysis on physiology integrity. Several parameters were compared between processed loaded with l-asparaginase ("eryaspase"), without non-processed RBCs. Processed less hydrated displayed a reduction intracellular...
Soft materials such as polymer gels, synthetic biomaterials and living biological tissues are generally classified viscoelastic or viscoplastic materials, because they behave neither pure elastic solids, nor viscous fluids. When stressed beyond their linear regime, cross-linked biopolymer gels can nonlinearly (inelastically) up to failure. In cells, this type of behavior is more frequent cytoskeleton basically made chains with very different structural flexibility properties. These networks...
Under physiological and pathological conditions, cells experience large forces deformations that often exceed the linear viscoelastic regime. Here we drive CD34+ isolated from healthy leukemic bone marrows in highly nonlinear elasto-plastic regime, by poking their perinuclear region with a sharp AFM cantilever tip. We use wavelet transform mathematical microscope to identify singular events force-indentation curves induced local rupture cytoskeleton (CSK). distinguish two types of events,...
The internal distribution of refractive indices (RIs) a living cell is much more complex than usually admitted in multi-shell models. reconstruction RI maps from single phase images has rarely been achieved for several reasons: (i) we still have very little knowledge the impact macromolecular complexes on local and (ii) changes produced by light propagation through sample are mixed with diffraction effects bodies. We propose implementation 2D wavelet-based contour chain detection method to...
Abstract Background: Increasing evidence suggests that gut microbiome composition modulates tumor response to therapies, including immune checkpoint inhibitors (ICI). Clinical proofs of concept were obtained in pilot studies using healthy or ICI-responder donor-derived fecal matter transplants modulate the non-responding cancer patients and improve their ICI. These results support development Microbiome Ecosystem TherapiesTM (MET) replicating at large industrial scale effects ICI-responders...
<h3>Background</h3> Increasing evidence suggests that gut microbiome composition modulates tumor response to therapies, including immune checkpoint inhibitors (ICI). Clinical proofs of concept were obtained in pilot studies using healthy or ICI-responder donor-derived fecal matter transplants modulate the non-responding cancer patients and improve their ICI.<sup>1–3</sup> These results support development Microbiome Ecosystem Therapies<sup>TM</sup> (MET) replicating at large industrial scale...
We propose a three-dimensional (3D) imaging platform based on lens-free microscopy to perform multi-angle acquisitions 3D cell cultures embedded in extracellular matrix (ECM). developed algorithms the Fourier diffraction theorem fully reconstructions of biological samples and we adapted microscope incubator conditions. Here demonstrate for first time, 3D+time culture over 8 days directly into incubator. The reconstructed volume is as large ~5 mm<sup>3</sup> provides unique way observe same...
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Les therapies traditionnelles du cancer, chimio- et radiotherapie, presentent de nombreux inconvenients car ces traitements ne sont pas specifiques des cellules tumorales donc egalement deleteres envers les saines. La resistance, conduisant a la rechute chez patients, pourrait etre causee par presence souches cancereuses (CSC), une population presente au sein tumeur possedant, comme classiques, proprietes d’auto-renouvellement differenciation. Ces seraient impliquees dans l’initiation...