A5009344154- Glycosylation and Glycoproteins Research
- Viral-associated cancers and disorders
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Escherichia coli research studies
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related Molecular Pathways
- Chronic Lymphocytic Leukemia Research
- Toxin Mechanisms and Immunotoxins
- Cell death mechanisms and regulation
- Carbohydrate Chemistry and Synthesis
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Erythrocyte Function and Pathophysiology
- Galectins and Cancer Biology
- Blood groups and transfusion
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- Autophagy in Disease and Therapy
- Immunotherapy and Immune Responses
- Protein Degradation and Inhibitors
- Adenosine and Purinergic Signaling
- Cytomegalovirus and herpesvirus research
- Sarcoma Diagnosis and Treatment
Institut Gustave Roussy
2014-2024
Centre National de la Recherche Scientifique
2014-2024
Université Paris-Saclay
2017-2024
Laboratoire d'études sur les monothéismes
1983-2023
Université Paris-Sud
2008-2019
Transnet (South Africa)
2006
National Institute of Neurological Disorders and Stroke
1998
National Institutes of Health
1998
National Cancer Institute
1998
Département Microbiologie et Chaîne Alimentaire
1998
The antigen defined by a rat monoclonal antibody directed to Burkitt lymphoma cell line was identified as globotriaosylceramide [Galα(1 → 4)-Galβ(1 4)-Glcβ(1 1)-ceramide]. demonstrated strict steric specificity since it did not react with globoisotriaosylceramide 3)-Galβ(1 1)-ceramide], the positional isomer of associated lymphoma. Chemical analysis various lines revealed that cells contained more than 100 times much glycolipid found in other human and leukemia lines.
Abstract We have previously reported that a neutral glycolipid (globotriaosylceramide; Gb 3 ) was specifically expressed on Burkitt's lymphoma cells and subset of germinal center tonsillar B lymphocytes. Recently the molecule recognized as new cell differentiation antigen now defines CD77 cluster. Here we report an extensive phenotypic functional characterization + These low buoyant density are thus purified using Percoll gradient. They express various antigens such CD19, CD20, CD21, CD22...
To examine whether globotriaosylceramide (Gb3/CD77) is a receptor for verotoxins (VTs) in vivo, sensitivity of Gb3/CD77 synthase null mutant mice to VT-2 and VT-1 was analyzed. Although wild-type died after administration 0.02 microg or 1.0 VT-1, the showed no reaction doses as much 100 times that administered wild types. Expression analysis mouse tissues with antibody revealed low, but definite, levels were expressed microvascular endothelial cells brain cortex pia mater renal tubular...
A monoclonal antibody, referred to as 38.13, was obtained by fusing murine myeloma cells with Lewis rat splenocytes sensitized Daudi (human Burkitt lymphoma containing Epstein--Barr virus genome but lacking HLA-A, -B, and -C beta 2-microglobulin molecules at the cell surface). 38.13 antibody demonstrated be a IgM. By complement-dependent microcytotoxicity indirect immunofluorescence assays, shown react specifically derived from tumors, including both genome-carrying virus-negative lymphoma....
The expression of the H-Y antigen has been tested on several human lymphoid lines and mouse teratocarcinoma cell during differentiation. male line Raji is a very useful target for studies by lymphocytotoxicity test with rat anti-H-Y sera. With few exceptions, all cells carrying Y chromosome were positive. One exceptions Daudi which, besides lacking antigen, also lacks beta2-microglobulin. We have studied possible association between beta2-microglobulin, HLA redistribution experiments....
The expression cloning of a cDNA for globotriaosylceramide (Gb3)/CD77 synthase (α1,4-galactosyltransferase) was achieved using an anti-Gb3 antibody and mouse L cells as recipient cell line the transfection. isolated clone designated pVTR1 predicted type II membrane protein with 19 amino acids cytoplasmic domain, 26 transmembrane region, catalytic domain 308 acids. Introduction into resulted in neosynthesis Gb3/CD77, extracts transfectant showed α1,4-galactosyltransferase activity only on...
Natural killer (NK) cell activity toward K562 target cells and antibody-dependent cell-mediated cytotoxicity (ADCC) L1210 sensitized with anti-L1210 antisera were sequentially tested in peripheral blood lymphocytes (PBLs) from 24 human bone marrow (BM) recipients. Although consistently decreased before the transplant, NK was restored all of patients that argues for a origin progenitors humans. In without graft-versus-host disease (GVHD), remained low during 1st month after then rapidly...
Viral gene sequences from an enlarged set of about 200 Epstein-Barr virus (EBV) strains, including many primary isolates, have been used to investigate variation in key viral genetic regions, particularly LMP1, Zp, gp350, EBNA1, and the BART microRNA (miRNA) cluster 2. Determination type 1 2 EBV saliva samples people a wide range geographic ethnic backgrounds demonstrates small percentage healthy white Caucasian British carrying predominantly EBV. Linkage Zp gp350 variants is likely be due...
The molecular genetic basis of the P histo-blood group system has eluded characterization despite extensive studies biosynthesis P<sub>1</sub>, P, and P<sup>k</sup>glycolipids. main controversy been whether a single or two distinct UDP-Gal:Galβ1-R 4-α-galactosyltransferases catalyze syntheses structurally related P<sub>1</sub> P<sup>k</sup>antigens. polymorphism is linked to 22q11.3-ter. Data base searches with coding region an α4GlcNAc-transferase identified novel homologous gene at 22q13.2...
Previously, we showed that the addition of human erythrocyte glycosphingolipids (GSLs) to nonhuman CD4 + or GSL-depleted cells rendered those susceptible HIV-1 envelope glycoprotein-mediated cell fusion. Individual components in GSL mixture were isolated by fractionation on a silica-gel column and incorporated into membranes cells. GSL-supplemented target then examined for their ability fuse with TF228 expressing LAI glycoprotein. We found one fraction, fraction 3, exhibited highest recovery...
Abstract In our previous study, a Burkitt lymphoma-associated antigen defined by monoclonal antibody, designated 38.13, was characterized as globotriaosylceramide (Gb3, Gal alpha 1----4 beta Glc 1----1 Cer) (Nudelman, E., Kannagi, R., Hakomori, S., Parsons, M., Lipinski, Wiels, J., Fellous, and Tursz, T. (1983) Science (Wash. D.C.) 220, 509-511). Consequently, we have studied the enzymatic basis organization of Gb3 expression in compared with non-Burkitt lymphoblastoid cell lines. lymphoma...
Additional chromosomal abnormalities are currently detected in Burkitt's lymphoma. They play major roles the progression of BL and prognosis. The genes involved remain elusive. A whole-genome oligonucleotide array CGH analysis correlated with karyotype FISH was performed a set 27 lymphoma-derived cell lines primary tumors. More than half 145 CNAs<2 Mb were mapped to Mendelian CNVs, including GSTT1, glutathione s-transferase BIRC6, an anti-apoptotic protein, possibly predisposing some...
Abstract In the hematopoietic system CD77, a glycolipid surface antigen, is restricted to group I Burkitt's lymphoma (BL) cell lines and subset of germinal center B lymphocytes. Recently, we have reported that recombinant subunits Verotoxin, which specifically binds induce programmed death CD77+ BL cells. Here, show an anti-CD77 monoclonal antibody (38.13) immobilized on tissue culture dishes also induces apoptosis, explored signal transducing events leading this death. We ligation CD77...
The spatiotemporal program of metazoan DNA replication is regulated during development and altered in cancers. We have generated novel OK-seq, Repli-seq RNA-seq data to compare the gene expression programs twelve cancer non-cancer human cell types. Changes fork directionality (RFD) determined by OK-seq are widespread but more frequent within GC-poor isochores largely disconnected from transcription changes. Cancer RFD profiles cluster with cells similar developmental origin not different...
Abstract Burkitt lymphoma (BL) is a B cell malignancy associated with the Epstein−Barr virus (EBV). Most BL cases are characterized by t(8;14) chromosomal translocation involving MYC oncogene and immunoglobulin heavy chain gene ( IGH ). The role of EBV in promoting this remains largely unknown. Here we provide experimental evidence that reactivation from latency leads to an increase proximity between loci, otherwise located far away nuclear space both B‐lymphoblastoid lines patients'...
Globotriasosylceramide (Gb3), a neutral glycosphingolipid, is the B-cell differentiation antigen CD77 and acts as receptor for most Shiga toxins, including verotoxin-1 (VT-1). We have shown that both anti-Gb3/CD77 mAb VT-1 induce apoptosis in Burkitt's lymphoma cells. compared apoptotic pathways induced by these two molecules selecting cell lines sensitive to only one of inducers or both. In all (including apoptosis-resistant line), was transported endoplasmic reticulum inhibited protein...
Abstract Monoclonal antibody 38.13 was raised against the Burkitt lymphoma cell line Daudi and has been previously shown to recognize a globotriaosylceramide (p k ) determinant expressed selectively on few other B‐cell lymphomas. We report here that binds subset of normal B lymphocytes in germinal centres secondary lymphoid follicles as well blood vessels. In malignant populations equivalent various developmental compartments lineage, only those with follicular phenotype express p at...