A5026297937- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Galectins and Cancer Biology
- Escherichia coli research studies
- Monoclonal and Polyclonal Antibodies Research
- Microbial Metabolites in Food Biotechnology
- Bacterial Genetics and Biotechnology
- Biofuel production and bioconversion
- Complement system in diseases
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- Lipid Membrane Structure and Behavior
- Plant Pathogenic Bacteria Studies
- Antifungal resistance and susceptibility
- Enzyme Catalysis and Immobilization
- Studies on Chitinases and Chitosanases
- Caveolin-1 and cellular processes
- Antimicrobial Peptides and Activities
- Transgenic Plants and Applications
- Polysaccharides and Plant Cell Walls
- Toxin Mechanisms and Immunotoxins
Centre National de la Recherche Scientifique
2016-2025
Université Grenoble Alpes
2016-2025
Centre de Recherches sur les Macromolécules Végétales
2013-2024
Université Joseph Fourier
2008-2014
Institut de Chimie Moléculaire de Grenoble
2010-2013
Mansfield University
2012
European Molecular Biology Laboratory
1999-2010
University of York
1998-2005
University of British Columbia
1998-2004
Northwestern University
2004
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved synthesis, modification degradation complex carbohydrates. is closely connected with actively curated CAZy database, which provides sequence-based foundation for biochemical, mechanistic structural characterization these diverse proteins. Now celebrating its 10th anniversary online, successful example dynamic,...
The enzymatic hydrolysis of O-glycosidic linkages is one the most diverse and widespread reactions in nature involves a classic "textbook" enzyme mechanism. A multidisciplinary analysis beta-glycoside hydrolase, Cel5A from Bacillus agaradhaerens, presented which structures each native, substrate, covalent-intermediate, product complexes have been determined their interconversions analyzed kinetically, providing unprecedented insights into mechanism this class. Substrate bound distorted 1S3...
The opportunistic Gram-negative bacterium Pseudomonas aeruginosa is a leading pathogen for infections of immuno-compromised patients and those suffering from cystic fibrosis. Its ability to switch planktonic life aggregates, forming the so-called biofilms, front-line mechanism antimicrobial resistance. bacterial carbohydrate-binding protein LecB an integral component necessary biofilm formation. Here, we report new class drug-like low molecular weight inhibitors lectin with nanomolar...
Among the numerous well-characterized families of glycosidases, family 4 appears to be anomaly, requiring both catalytic NAD+ and a divalent metal for activity. The unusual cofactor requirement prompted proposal mechanism involving key NAD+-mediated redox steps as well elimination glycosidic oxygen. Primary kinetic isotope effects 2- 3-deutero substrate analogues, isotopic exchange with solvent, structural analysis 6-phospho-β-glucosidase, BglT (E.C. 3.2.1.6), provided evidence in support...
Burkholderia ambifaria is generally associated with the rhizosphere of plants where it has biocontrol effects on other microorganisms. It also a member cepacia complex, group closely related bacteria that cause lung infections in immunocompromised patients as well granulomatous disease or cystic fibrosis. Our previous work indicated fucose human epithelia frequent target for lectins and adhesins pathogens (Sulák, O., Cioci, G., Lameignère, E., Balloy, V., Round, A., Gutsche, I., Malinovská,...
Aspergillus fumigatus is an important allergen and opportunistic pathogen. Similarly to many other pathogens, it able produce lectins that may be involved in the host-pathogen interaction. We focused on lectin AFL, which was prepared recombinant form characterized. Its binding properties were studied using hemagglutination glycan array analysis. determined specificity of towards l-fucose fucosylated oligosaccharides, including α1-6 linked core-fucose, marker for cancerogenesis. Other...
Three small families of hydrolytically stable thioaryl glycosides were prepared as inhibitors the LecA (PA-IL) virulence factor corresponding to carbohydrate binding lectin from bacterial pathogen Pseudomonas aeruginosa. The monosaccharidic arylthio β-D-galactopyranosides served a common template for major series that was also substituted at O-3 position. Arylthio disaccharides lactose and melibiose constituted other two members. In spite fact natural ligand is glycolipid...
Abstract Lectin LecA is a virulence factor of Pseudomonas aeruginosa involved in lung injury, mortality, and cellular invasion. Ligands competing with human glycoconjugates for binding are thus promising candidates to counteract P. infections. We have identified novel divalent ligand from focused galactoside(Gal)‐conjugate array which binds very high affinity ( K d =82 n M ). Crystal structures complexed the together modeling studies confirmed its ability chelate two sites LecA. The lowers...
The treatment of infections due to the opportunistic pathogen Pseudomonas aeruginosa is often difficult, as a consequence bacterial biofilm formation. Such protective environment shields bacterium from host defense and antibiotic secures its survival. One crucial factor for maintenance architecture carbohydrate-binding lectin LecB. Here, we report identification potent mannose-based LecB inhibitors screening four series mannosides in novel competitive binding assay Cinnamide sulfonamide...
Biofilm formation by pathogenic bacteria is a hallmark of chronic infections. In many cases, lectins play key roles in establishing biofilms. The pathogen Pseudomonas aeruginosa often exhibiting various drug resistances employs its LecA and LecB as virulence factors biofilm building blocks. Therefore, inhibition the function these proteins thought to have potential developing "pathoblockers" preventing virulence. A covalent lectin inhibitor specific carbohydrate binding site described for...
Biofilm formation is a key mechanism of antimicrobial resistance. We have recently reported two classes orally bioavailable C-glycosidic inhibitors the Pseudomonas aeruginosa lectin LecB with antibiofilm activity. They proved efficient in target binding, were metabolically stable, nontoxic, selective, and potent inhibiting bacterial biofilm. Here, we designed synthesized six new carboxamides 24 sulfonamides for detailed structure-activity relationship clinically representative variants....
Chronic colonization of the lungs by opportunist bacteria such as Pseudomonas aeruginosa and members Bcc (Burkholderia cepacia complex) is major cause morbidity mortality among CF (cystic fibrosis) patients. PA-IIL (lecB gene), a soluble lectin from Ps. aeruginosa, has been subject much interest because its very strong affinity for fucose. Orthologues have identified in Ralstonia solanacearum, Chromobacterium violaceum Burkholderia Bcc. The genome J2315 strain B. cenocepacia, responsible...
Abstract Lectins are glycan‐binding proteins that involved in the recognition of glycoconjugates at cell surface. When binding to glycolipids, multivalent lectins can affect their distribution and alter membrane shapes. Neolectins have now been designed with controlled number position sites decipher role multivalency on avidity a glycosylated surface dynamics glycolipids. A monomeric hexavalent neolectin has first engineered from trimeric bacterial lectin, From this template, 13 different...
The sequence of the virulence factor LecB differs significantly between evolutionarily diverged PAO1- or PA14-like strains and can serve as marker for strain classification. Despite these variations, its comparable ligand selectivity makes a highly promising target anti-virulence therapy.
The mechanisms of crystalline cellulose degradation by cellulases are paramount importance for the exploitation these enzymes in applied processes, such as biomass conversion. Cellulases have traditionally been classified into cellobiohydrolases, which effective materials, and endoglucanases, appear to act on "soluble" regions substrate. Humicola insolens Cel6A (CBH II) is a cellobiohydrolase from glycoside hydrolase family 6 whose native structure has determined at 1.9 Å resolution [Varrot,...
Glycosidases are some of the most ubiquitous enzyme in nature. Their biological significance, coupled to their enormous catalytic prowess derived from tight binding transition state, is reflected importance as therapeutic targets. Many glycosidase inhibitors known. Imino sugars often potent inhibitors, yet many facets mode action, such degree, if any, transition-state "mimicry" and protonation state when bound target remain unclear. Atomic resolution analysis endoglucanase, Cel5A, complex...
Abstract Polyelectrolyte‐multilayer microcapsules are made by layer‐by‐layer (LbL) assembly of oppositely charged polyelectrolytes onto sacrificial colloidal particles, followed core removal. In this paper, contact‐killing polyelectrolyte prepared based solely on polysaccharides. To end, water‐soluble quaternized chitosan (QCHI) with varying degrees substitution (DS) and hyaluronic acid (HA) assembled into thin films. The quaternary ammonium groups selectively grafted the primary amine group...