A5050574613- Enzyme Production and Characterization
- Carbohydrate Chemistry and Synthesis
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Toxin Mechanisms and Immunotoxins
- Peptidase Inhibition and Analysis
- Polysaccharides and Plant Cell Walls
- Studies on Chitinases and Chitosanases
- Biochemical and Molecular Research
- Amino Acid Enzymes and Metabolism
- Freezing and Crystallization Processes
- Fungal and yeast genetics research
- Venomous Animal Envenomation and Studies
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Structural Characterization
- Microtubule and mitosis dynamics
- Magnetic and Electromagnetic Effects
- Cellular transport and secretion
- Protein Structure and Dynamics
- Marine Toxins and Detection Methods
- Macrophage Migration Inhibitory Factor
Tohoku University
2013-2025
The University of Tokyo
2004-2017
Food Research Institute
2006-2010
National Agriculture and Food Research Organization
2009-2010
Ishikawa Prefectural University
2009
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved synthesis, modification degradation complex carbohydrates. is closely connected with actively curated CAZy database, which provides sequence-based foundation for biochemical, mechanistic structural characterization these diverse proteins. Now celebrating its 10th anniversary online, successful example dynamic,...
β-Glucosidase from Kluyveromyces marxianus (KmBglI) belongs to the GH3 (glycoside hydrolase family 3). The enzyme is particularly unusual in that a PA14 domain (pf07691), for which carbohydrate-binding role has been claimed, inserted into catalytic core sequence. In present study, we determined enzymatic properties and crystal structure of KmBglI complex with glucose at 2.55 A (1 A=0.1 nm) resolution. striking characteristic was activity essentially limited disaccharides, when trisaccharides...
Wood ash, a byproduct of woody biomass power generation, has potential as an alternative K fertilizer due to its high content and pH-raising properties. However, concerns remain about heavy metal contaminants like Cr the limited understanding element dynamics in soil–solution–crop systems after wood ash’s application. This study examined effects 1% (w/w) ash on komatsuna (Brassica rapa var. perviridis) uptake low-K soil through pot experiment. XRD was used analyze mineral composition,...
Cellobiose phosphorylase, a member of the glycoside hydrolase family 94, catalyses reversible phosphorolysis cellobiose into α-D-glucose 1-phosphate and D-glucose with inversion anomeric configuration. The substrate specificity reaction mechanism phosphorylase from Cellvibrio gilvus have been investigated in detail. We determined crystal structure glucose-sulphate glucose-phosphate complexes this enzyme at maximal resolution 2.0 Å (1 Å=0.1 nm). phosphate ion is strongly held through several...
The tyrosine residue Y198 is known to support a nucleophilic water molecule with the general base residue, D263, in reducing-end xylose-releasing exo-oligoxylanase (Rex). A mutation changing it into phenylalanine caused drastic decrease hydrolytic activity and small increase F− releasing from α-xylobiosyl fluoride presence of xylose. In contrast, mutations at D263 resulted decreased activity. As result high low activity, Y198F Rex accumulates large amount product during glycosynthase...
Galacto-N-biose/lacto-N-biose I phosphorylase (GLNBP) from Bifidobacterium longum, a key enzyme for intestinal growth, phosphorolyses galacto-N-biose and lacto-N-biose with anomeric inversion. GLNBP homologues are often found in human pathogenic commensal bacteria, their substrate specificities potentially define the nutritional acquisition ability of these microbes habitat. We report crystal structures five different ligand-binding forms. This is first three-dimensional structure glycoside...
Enhancing the thermostability of cellulose-degrading enzymes is pivotal for establishing an efficient bioconversion system from cellulosic materials to value-added compounds. Here, by introducing random and saturation mutagenesis into Thermoascus aurantiacus β-glucosidase gene, we generated a hyperthermostable mutant with five amino acid substitutions. Analysis temperature-induced unfolding revealed involvement each replacement in increased Tm value. Structural analysis showed that all...
Softening of winter-harvested frozen broccoli is a challenge. Application boron (B) can improve fruit texture, but its effects on processed vegetables remain unexplored. This study the first to investigate impact preharvest B spraying [0 (unsprayed), 195, 390, and 585 mg L-1] firmness microstructure broccoli, utilizing different cultivars harvested during winter season. In unsprayed group, 'Pixel' florets exhibited highest firmness, followed by 'Ohayo', 'Konnichiwa', 'Kombanwa', depending...
The peptidyl prolyl cis/trans isomerase Pin1 enhances the uptake of triglycerides and differentiation fibroblasts into adipose cells in response to insulin stimulation. downregulation could be a potential approach prevent treat obesity-related disorders. In order identify an inhibitor that exhibited minimal cytotoxicity, we established high-throughput screen for inhibitors used this method from 1,056 crude fractions two natural product libraries. candidate, phlorotannin called 974-B, was...
Summary The soybean endosymbiont B radyrhizobium japonicum is able to scavenge the greenhouse gas N 2 O through reductase ( os). In previous research, emission from rhizosphere was mitigated by . os ++ strains (mutants with increased activity). Here, we report mechanism underlying phenotype. Comparative analysis of mutant genomes showed that mutation bll4572 resulted in encodes NasS , nitrate NO 3 − )‐sensor two‐component NasST regulatory system. Transcriptional analyses nos Z (encoding os)...
Neurons undergo several morphological changes as a part of normal neuron maturation process. Alzheimer disease is associated with increased neuroproliferation and impaired neuronal maturation. In this study, we demonstrated that Gas7b (growth arrest specific protein 7b) expression in cell line, Neuro 2A, induces by facilitating formation dendrite-like processes and/or filopodia projections co-localizes neurite microtubules. Molecular analysis was performed to evaluate whether associates...
Amyloid-β peptides (Aβs) are produced via cleavage of the transmembrane region amyloid precursor protein (APP) by γ-secretase and responsible for Alzheimer's disease. Familial disease (FAD) is associated with APP mutations that disrupt reaction increase production neurotoxic Aβs, i.e., Aβ42 Aβ43. Study activate restore FAD mutants necessary to understand mechanism Aβ production. In this study, using a yeast reconstruction system, we revealed one mutations, T714I, severely reduced cleavage,...
The emergence of coronavirus disease 2019 (COVID-19) posed a major challenge to healthcare systems worldwide, especially as mutations in the culprit Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) complicated development vaccines and antiviral drugs. Therefore, search for natural products with broad anti-SARS-CoV-2 capabilities is an important option prevention treatment similar infectious diseases. Lectins, which are widely recognized agents, could contribute This study...
2-Hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase (CumD) from Pseudomonas fluorescens IP01 hydrolyzes a meta-cleavage product generated in the cumene (isopropylbenzene) degradation pathway. The crystal structures of inactive S103A mutant CumD enzyme complexed with isobutyrate and acetate ions were determined at 1.6 2.0 A resolution, respectively. located same position active site, occupied site for part hydrolysis CumD, which has key determinant group substrate specificity related...
Journal Article Structural explanation for the acquisition of glycosynthase activity Get access Masafumi Hidaka, Hidaka 1Department Biotechnology, The University Tokyo, Japan; 2National Food Research Institute, National Agriculture and Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, 3Ishikawa Prefectural University, Nonoichi, Ishikawa, Japan Search other works by this author on: Oxford Academic PubMed Google Scholar Shinya Fushinobu, Fushinobu Yuji Honda, Honda Takayoshi Wakagi,...
Nitrate (NO3−) and nitrite (NO2−) are the physiological sources of nitric oxide (NO), a key biological messenger molecule. NO3−/NO2− exerts beneficial impact on NO homeostasis its related cardiovascular functions. To visualize dynamics for assessing precise roles these anions, we developed genetically encoded intermolecular fluorescence resonance energy transfer (FRET)-based indicator, named sNOOOpy (sensor in physiology), by employing NO3−/NO2−-induced dissociation NasST involved...
A novel series of C12-keto-type saxitoxin (STX) derivatives bearing an unusual nonhydrated form the ketone at C12 has been synthesized, and their NaV -inhibitory activity evaluated in a cell-based assay as well whole-cell patch-clamp recording. Among these compounds, 11-benzylidene STX (3 a) showed potent inhibitory against neuroblastoma Neuro 2A both electrophysiological analyses, with EC50 IC50 values 8.5 30.7 nm, respectively. Interestingly, compound tetrodotoxin-resistant subtype 1.5,...
fungi contain α-1,3-glucan with a low proportion of α-1,4-glucan as major cell wall polysaccharide. Glycosylphosphatidylinositol (GPI)-anchored α-amylases are conserved in