A5041111651- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Microbial Applications in Construction Materials
- Seaweed-derived Bioactive Compounds
- Enzyme Structure and Function
- Polysaccharides and Plant Cell Walls
- Immunodeficiency and Autoimmune Disorders
- Studies on Chitinases and Chitosanases
- Echinoderm biology and ecology
- Infant Nutrition and Health
- Diet, Metabolism, and Disease
- Calcium Carbonate Crystallization and Inhibition
- Monoclonal and Polyclonal Antibodies Research
- Advanced Cellulose Research Studies
- Polyamine Metabolism and Applications
- Corrosion Behavior and Inhibition
- Plant Pathogens and Fungal Diseases
- Lysosomal Storage Disorders Research
- Marine and coastal plant biology
- Bacterial biofilms and quorum sensing
- Microbial Metabolic Engineering and Bioproduction
Petersburg Nuclear Physics Institute
2016-2025
Kurchatov Institute
2014-2024
Peter the Great St. Petersburg Polytechnic University
2014-2020
Russian Academy of Sciences
2001-2010
KTH Royal Institute of Technology
2007
North Carolina State University
2007
AlbaNova
2007
Universidade de São Paulo
2002-2005
Universidade Estadual de Campinas (UNICAMP)
2002
Institute of Molecular Biology and Biophysics
1997
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved synthesis, modification degradation complex carbohydrates. is closely connected with actively curated CAZy database, which provides sequence-based foundation for biochemical, mechanistic structural characterization these diverse proteins. Now celebrating its 10th anniversary online, successful example dynamic,...
Fucoidans, sulfated polysaccharides found in cell walls of brown algae, are considered as a promising antimicrobial component for various applications medicine and the food industry. In this study, we compare antibacterial properties two fractions fucoidan from algae Fucus vesiculosus gathered littoral Barents Sea sampled at different stages purification. The crude fraction was isolated by extraction with aqueous ethanol sonication. purified obtained additional treatment solution calcium...
The enzyme alpha-galactosidase (alpha-GAL, also known as alpha-GAL A; E.C. 3.2.1.22) is responsible for the breakdown of alpha-galactosides in lysosome. Defects human lead to development Fabry disease, a lysosomal storage disorder characterized by buildup alpha-galactosylated substrates tissues. an active target clinical research: there are currently two treatment options recombinant replacement therapy (approved United States 2003) and pharmacological chaperone (currently trials)....
Organization of glycoside hydrolase (GH) families into clans expands the utility information on catalytic mechanisms member enzymes. This issue was examined for GH27 and GH36 through biochemical analysis α-galactosidase from Thermotoga maritima (TmGalA). Catalytic residues in TmGalA were inferred structural homology with members to facilitate design site-directed mutants. Product confirmed that wild type (WT) acted retention anomeric stereochemistry, analogous Conserved acidic kinetic D327G...
Abstract To date, the mechanisms of biomineralization induced by bacterial cells in context biofilm formation remain subject intensive studies. In this study, we analyzed influence medium components on induction CaCO 3 precipitation Bacillus cereus and composition extracellular matrix (ECM) formed submerged culture. While accumulation polysaccharides amyloids appeared to be independent presence calcium urea during growth, DNA (eDNA), as well carbonate, required both ingredients medium....
Lactoferrin (LF) is a Fe 3+ ‐binding glycoprotein, first recognized in milk and then other human epithelial secretions barrier fluids. Many different functions have been attributed to LF, including protection from iron‐induced lipid peroxidation, immunomodulation cell growth regulation, DNA binding, transcriptional activation. Its physiological role still unclear, but it has suggested be responsible for primary defense against microbial viral infection. We present evidence that subfractions...
Extracellular exo-inulinase has been isolated from a solid-phase culture of the filamentous fungus Aspergillus awamori var. 2250. The apparent molecular mass monomer enzyme was 69±1kDa, with pI 4.4 and pH optimum 4.5. hydrolysed β-(2 → 1)-fructan (inulin) 6)-fructan (levan) via exo-cleavage, releasing fructose. values for Michaelis constants Km Vmax in hydrolysis inulin were 0.003±0.0001mM 175±5μmol·min−1·mg−1. same parameters levan 2.08±0.04mg/ml 1.2±0.02μmol/min per mg, respectively. gene...
The ascomycete Geotrichum candidum is a versatile and efficient decay fungus that involved, for example, in biodeterioration of compact discs; notably, the 3C strain was previously shown to degrade filter paper cotton more efficiently than several industrial enzyme preparations. Glycoside hydrolase (GH) family 7 cellobiohydrolases (CBHs) are primary constituents cellulase cocktails employed biomass conversion, feature tunnel-enclosed active sites enable processive hydrolytic cleavage...
The ascomycete fungus Trichoderma reesei is the predominant source of enzymes for industrial conversion lignocellulose. Its glycoside hydrolase family 7 cellobiohydrolase (GH7 CBH) TreCel7A constitutes nearly half enzyme cocktail by weight and major workhorse in cellulose hydrolysis process. orthologs from atroviride (TatCel7A) harzianum (ThaCel7A) show high sequence identity with TreCel7A, ~ 80%, represent naturally evolved combinations cellulose-binding tunnel-enclosing loop motifs, which...
Plant cell enlargement is coupled to dynamic changes in wall composition and properties. Such rearrangements are provided, besides the differential synthesis of individual components, by enzymes that modify polysaccharides muro. To reveal may contribute these modifications relate them stages elongation growth grasses, we carried out a transcriptomic study five zones primary maize root. In initiation elongation, significant occur with xyloglucan: once synthesized meristem, it can be linked...
The technology of fucoidan extraction significantly affects its properties. This study aimed to evaluate the impact dynamic maceration (DM) and ultrasound-assisted (UAE) on antioxidant anticancer properties from Arctic brown algae. Fucus vesiculosus (Fv), serratus (Fs), distichus (Fd), Ascophyllum nodosum (An) were collected Barents Sea. average yield uronic acid was higher (by 43.2% 22.0%, respectively) after UAE, while phlorotannin content decreased by 53.7% compared with DM. fucose level...
Microbially induced CaCO3 precipitation (MICP) is considered as an alternative green technology for cement self-healing and a basis the development of new biomaterials. However, some issues about role bacteria in induction biogenic crystal nucleation, growth aggregation are still debatable. Our aims were to screen ureolytic calcifying microorganisms analyze their MICP abilities during urea-supplemented urea-deficient media. Nine candidates showed high level urease specific activity, sharp...
Extracellular exo-inulinase has been isolated from a solid-phase culture of the filamentous fungus Aspergillus awamori var. 2250. The apparent molecular mass monomer enzyme was 69±1kDa, with pI 4.4 and pH optimum 4.5. hydrolysed β-(2 → 1)-fructan (inulin) 6)-fructan (levan) via exo-cleavage, releasing fructose. values for Michaelis constants Km Vmax in hydrolysis inulin were 0.003±0.0001mM 175±5μmol·min−1·mg−1. same parameters levan 2.08±0.04mg/ml 1.2±0.02μmol/min per mg, respectively. gene...