A5069943013- Fungal and yeast genetics research
- Fungal Biology and Applications
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- Mycorrhizal Fungi and Plant Interactions
- Mycotoxins in Agriculture and Food
- Plant and fungal interactions
- Fungal Plant Pathogen Control
- Enzyme-mediated dye degradation
- Plant Pathogens and Fungal Diseases
- Antifungal resistance and susceptibility
- Antibiotics Pharmacokinetics and Efficacy
- Microbial Metabolic Engineering and Bioproduction
- Synthesis of Organic Compounds
- Phenothiazines and Benzothiazines Synthesis and Activities
- Polymer Surface Interaction Studies
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- Yeasts and Rust Fungi Studies
- Chemical synthesis and alkaloids
- Bacterial biofilms and quorum sensing
- Pharmacological Effects of Natural Compounds
Kyoto University
2003-2024
Tohoku University
2013-2024
Mirai Hospital
2024
Hatch (Canada)
2017-2022
University of Shizuoka
1995
Kyoto Pharmaceutical Industries (Japan)
1993
Summary Fungicides generally inhibit enzymatic reactions involved in fungal cellular biosynthesis. Here we report, for the first time, an example of fungicidal effects through hyperactivation a signal transduction pathway. The OSC1 gene, encoding MAP kinase (MAPK) related to yeast Hog1, was isolated from pathogen Colletotrichum lagenarium that causes cucumber anthracnose. osc1 knockout mutants were sensitive high osmotic stress and showed increased resistance fungicide fludioxonil,...
Cell wall integrity signaling (CWIS) maintains cell biogenesis in fungi, but only a few transcription factors (TFs) and target genes downstream of the CWIS cascade filamentous fungi are known. Because mitogen-activated protein kinase (MpkA) is key enzyme, transcriptional regulation mpkA wall-related (CWGs) important biogenesis. We cloned Aspergillus nidulans mpkA; rlmA, TF gene orthologous to Saccharomyces cerevisiae RLM1 that encodes Rlm1p, major Mpk1p-dependent regulates MPK1 besides CWGs;...
Oligosaccharides derived from cell wall of fungal pathogens induce host primary immune responses. To understand strategies circumventing the plant responses, polysaccharide localization was investigated using fluorescent labels during infectious structure differentiation in rice blast fungus Magnaporthe grisea. alpha-1,3-glucan labelled only on appressoria developing plastic surfaces, whereas it detected both germ tubes and surfaces. Chitin, chitosan beta-1,3-glucan were regardless...
Ustiloxins were found recently to be the first example of cyclic peptidyl secondary metabolites that are ribosomally synthesized in filamentous fungi. In this work, two function-unknown genes (ustYa/ustYb) gene cluster for ustiloxins from Aspergillus flavus experimentally involved cyclization peptide. Their homologous observed mainly fungi and mushrooms. They have "HXXHC" motifs might form active sites. Computational genome analyses showed these frequently located near candidate ribosomal...
ABSTRACT We previously reported that the group III histidine kinase Dic1p in maize pathogen Cochliobolus heterostrophus is involved resistance to dicarboximide and phenylpyrrole fungicides osmotic adaptation. In addition, exposure fungicide fludioxonil led improper activation of Hog1-type mitogen-activated protein kinases (MAPKs) some phytopathogenic fungi, including C. . Here we report, for first time, relationship between Hog1-related MAPK: a positive regulator MAPK filamentous fungi. The...
Although α-1,3-glucan is one of the major cell wall polysaccharides in filamentous fungi, physiological roles remain unclear. The model fungus Aspergillus nidulans possesses two synthase (AGS) genes, agsA and agsB. For functional analysis these we constructed several mutant strains A. nidulans: disruption, agsB double-disruption strains. We also CagsB which expression was controlled by inducible alcA promoter, with or without agsA-disrupting mutation. disruption did not show markedly...
The fungal high osmolarity glycerol (HOG) pathway is composed of a two-component system (TCS) and Hog1-type mitogen-activated protein kinase (MAPK) cascade. A group III (Nik1-type) histidine plays major role in the HOG several filamentous fungi. In this study, we characterized kinase, NikA/TcsC, life-threatening pathogenic fungus, Aspergillus fumigatus. deletion mutant nikA showed low conidia production, abnormal hyphae, marked sensitivity to stresses, resistance cell wall perturbing...
Chitin is an essential structural component of fungal cell walls composed transmembrane proteins called chitin synthases (CHSs), which have a large range reported effects in ascomycetes; however, are poorly understood agaricomycetes. In this study, evolutionary and molecular genetic analyses chs genes were conducted using genomic information from nine ascomycete six basidiomycete species. The results support the existence seven previously classified clades discovery three novel...
Under liquid culture conditions, the hyphae of filamentous fungi aggregate to form pellets, which reduces cell density and fermentation productivity. Previously, we found that loss α-1,3-glucan in wall fungus Aspergillus nidulans increased hyphal dispersion. Therefore, here constructed a mutant industrial A. oryzae three genes encoding synthase were disrupted (tripleΔ). Although tripleΔ not fully dispersed, strain did smaller pellets than wild-type strain. We next examined enzyme...
Filamentous fungi generally form aggregated hyphal pellets in liquid culture. We previously reported that α-1,3-glucan-deficient mutants of Aspergillus nidulans did not and their hyphae were fully dispersed, we suggested α-1,3-glucan functions aggregation. Yet, oryzae (AGΔ) still small pellets; therefore, hypothesized another factor responsible for forming remains these mutants. Here, identified an extracellular matrix polysaccharide galactosaminogalactan (GAG) as such a factor. To produce...
ABSTRACT Filamentous ascomycetous fungi possess many histidine kinases and two conserved response regulators, Ssk1p Skn7p, in their two-component signaling systems. We previously reported that the fungus unique group III kinase regulates high-osmolarity adaptation iprodione/fludioxonil fungicide sensitivity by controlling phosphorylation of Hog1-type mitogen-activated protein (MAPK) filamentous ascomycetes. Here, we have characterized regulator genes Ch Ssk1 Skn7 southern corn leaf blight...
α-1,3-Glucan is one of the main polysaccharides in cell wall filamentous fungi. Aspergillus nidulans has two α-1,3-glucan synthase genes, agsA and agsB. We previously revealed that AgsB a major vegetative hyphae, but function AgsA remained unknown because its low expression level lack phenotypic alteration upon gene disruption. To clarify role hyphal aggregation, we constructed strains overexpressing (agsAOE ) or agsB (agsBOE ), which other was disrupted. In liquid culture, wild-type agsBOE...
Abstract Hydrophobins, which are small-secreted proteins with both hydrophobic and hydrophilic parts, can self-assemble into an amphiphilic film at the air-water interface, helping fungus to form aerial hyphae. In agaricomycete Pleurotus ostreatus, more than 20 putative hydrophobin genes have been predicted. Of these, two genes, vmh2 vmh3, predominantly expressed in vegetative mycelium. this study, we focused on functions of Vmh2 Vmh3 mycelia. Based observation mycelial cross-section by...
Ustiloxin B, originally isolated from the fungus Ustilaginoidea virens, is a known inhibitor of microtubule assembly. B also produced by Aspergillus flavus and synthesized through ribosomal peptide synthesis pathway. In A. flavus, gene cluster associated with ustiloxin production contains 15 genes including those encoding fungal C6-type transcription factor precursor. Although koji mold oryzae, which genetically close to has corresponding cluster, it does not produce may be explained fact...