A5102021954- Enzyme Production and Characterization
- Peptidase Inhibition and Analysis
- Fungal and yeast genetics research
- Protein Hydrolysis and Bioactive Peptides
- Biofuel production and bioconversion
- Pancreatic function and diabetes
- Biochemical and Structural Characterization
- Studies on Chitinases and Chitosanases
- Phytase and its Applications
- Glycosylation and Glycoproteins Research
- Fungal Biology and Applications
- Neuropeptides and Animal Physiology
- Enzyme Structure and Function
- Pancreatic and Hepatic Oncology Research
- Chemical Synthesis and Analysis
- Microbial Natural Products and Biosynthesis
- Microbial Metabolites in Food Biotechnology
- Amino Acid Enzymes and Metabolism
- Protein Structure and Dynamics
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Ubiquitin and proteasome pathways
- Diabetes Management and Research
- Insect and Pesticide Research
- Uterine Myomas and Treatments
Tokyo University of Agriculture and Technology
2013-2024
Tohoku University
2005-2021
Nagasaki University Hospital
2019
Nagasaki University
2018
Yamaguchi University
2007-2009
Kyushu University
2006
Cell wall integrity signaling (CWIS) maintains cell biogenesis in fungi, but only a few transcription factors (TFs) and target genes downstream of the CWIS cascade filamentous fungi are known. Because mitogen-activated protein kinase (MpkA) is key enzyme, transcriptional regulation mpkA wall-related (CWGs) important biogenesis. We cloned Aspergillus nidulans mpkA; rlmA, TF gene orthologous to Saccharomyces cerevisiae RLM1 that encodes Rlm1p, major Mpk1p-dependent regulates MPK1 besides CWGs;...
BACKGROUNDEpigenetic regulation may be involved in modulation of gene expression during the normal cyclic changes human endometrium. We investigated DNA methyltransferases (DNMTs) endometrium menstrual cycle and influence sex steroid hormones on DNMT endometrial stromal cells (ESC) culture.
(1992). Nucleotide Sequence of the Subtilisin NAT Gene, aprN, Bacillus subtilis (natto) Bioscience, Biotechnology, and Biochemistry: Vol. 56, No. 11, pp. 1869-1871.
ABSTRACT Hydrophobic surface binding protein A (HsbA) is a secreted (14.5 kDa) isolated from the culture broth of Aspergillus oryzae RIB40 grown in medium containing polybutylene succinate- co -adipate (PBSA) as sole carbon source. We purified HsbA and determined its N-terminal amino acid sequence. found DNA sequence encoding whose N terminus matched that A. ozyzae genomic cloned hsbA cDNA constructed recombinant strain highly expressing . Orthologues were present animal pathogenic...
Awamori is a traditional distilled beverage made from steamed Thai-Indica rice in Okinawa, Japan. For brewing the liquor, two microbes, local kuro (black) koji mold Aspergillus luchuensis and awamori yeast Saccharomyces cerevisiae are involved. In contrast, that yeasts used for ethanol fermentation throughout world, characteristic of Japanese industries use molds as source enzymes maceration saccharification raw materials. Here we report draft genome mold, A. NBRC 4314 (RIB 2604). The total...
Uterine leiomyomas are the most common uterine tumors in women. Estrogen receptor-α (ER-α) is more highly expressed than normal myometrium, suggesting a link between and ER-α expression. DNA methylation an epigenetic mechanism of gene regulation plays important roles embryonic development disease progression including cancers. Here, we investigated status promoter region (−1188 to +229 bp) myometrium leiomyoma. By sodium bisulfite sequencing, 49 CpG sites proximal were shown be unmethylated...
Aberrant DNA methylation has been implicated in tumorigenesis. This study was undertaken to establish the genome-wide profile uterine leiomyomas and investigate whether status is altered leiomyomas. For this purpose, restriction landmark genomic scanning (RLGS) performed on a paired sample of leiomyoma adjacent normal myometrium. The RLGS revealed 29 aberrant spots (10 methylated 19 demethylated) comparison with One differently loci newly identified as GS20656 from human genome sequence...
Aspergillus oryzae PrtR is an ortholog of the transcription factor PrtT, which positively regulates extracellular peptidase genes in niger and fumigatus. To identify under control elucidate its regulatory mechanism A. oryzae, prtR gene disruption mutants were generated. The strain clearly showed a halo on media containing skim milk as nitrogen source, whereas ΔprtR formed smaller halo. Measurement acid activity revealed that approximately 84% acidic endopeptidase 86% carboxypeptidase...
ABSTRACT We cloned and characterized a novel Aspergillus nidulans histidine kinase gene, tcsB , encoding membrane-type two-component signaling protein homologous to the yeast osmosensor synthetic lethal N-end rule 1 ( SLN1 ), which transmits signals through high-osmolarity glycerol response HOG1 ) mitogen-activated (MAPK) cascade in cells environmental osmotic stimuli. From an A. cDNA library, we isolated positive clone containing 3,210-bp open reading frame that encoded putative consisting...
We isolated the kexB gene, which encodes a subtilisin-like processing enzyme, from filamentous fungus, Aspergillus oryzae. To examine physiological role of in A. oryzae, we constructed disruptant (DeltakexB), formed shrunken colonies with poor generation conidia on Czapek-Dox (CD) agar plates and hyperbranched mycelia CD liquid medium. The phenotypes DeltakexB strain were restored under high osmolarity both solid culture conditions. found that transcription mpkA putative mitogen-activated...
The clostridial collagenases G and H are multidomain proteins. For collagen digestion, the domain arrangement is likely to play an important role in binding hydrolysis. In this study, full-length collagenase protein from Clostridium histolyticum was expressed Escherichia coli purified. N-terminal amino acid of purified Ala31. showed enzymatic activity against azocoll as a substrate. To investigate Ca(2+) providing structural stability H, biophysical measurements were conducted using...
The role(s) of collagenase G (ColG) and H (ColH) during pancreatic islet isolation remains controversial, possibly due to the enzyme blends used in previous studies. We herein examined role ColG ColH using highly pure recombinant each subtype. Rat pancreases were digested thermolysin, together with ColG, ColH, or ColG/ColH (n = 9, respectively). No tryptic-like activity was detected any components blends. efficiency subtypes evaluated by yield function. Immunohistochemical analysis, vitro...
ABSTRACT The degradation of intracellular proteins by N-degron pathways depends on their N-terminal amino acids. In budding yeast, the Arg/N-degron pathway controls expression di/tri-peptide transporter gene degrading transcriptional repressor. However, there is no detailed information in filamentous fungi, and its role regulating microbial nitrogen metabolism unclear. Here, we demonstrated that E3 ubiquitin ligase, UbrA, which required for pathway, regulates peptidase fungus Aspergillus...
Aims: Prolyl aminopeptidase (PAP) degrades only amino-terminal proline from peptides. The food-grade fungus Aspergillus oryzae produces this enzyme in small amounts. In paper, we present efficient production of recombinant PAP with an overexpression system A. and characterization its biochemical properties. Methods Results: gene encoding was overexpressed as a His-tag fusion protein under taka-amylase (amyB) promoter limited expressing condition oryzae. activity the mycelia grown rich medium...
To elucidate correlative relationships between structural change and thermodynamic stability in proteins, a series of mutant human lysozymes modified at two buried positions (Ile56 Ile59) were examined. Their parameters denaturation crystal structures studied by calorimetry X-ray crystallography. The mutants 56 59 exhibited different responses to amino acid substitutions. changes due substitutions showed linear correlation with hydrophobicity substituted residues, having slopes each mutation...
Islet isolation currently requires collagenase, neutral protease and other components. Thermolysin (TL) from Bacillus thermoproteolyticus is the gold standard protease. However, we speculated that derived Clostridium histolyticum (Ch; ChNP) would be biologically superior for islet isolation. Tryptic-like activity has also been reported to important. Therefore, focused on clostripain (CP), since it one of main proteases in which possesses tryptic-like activity. We then examined synergistic...
Aspartyl aminopeptidase (DAP) has a high degree of substrate specificity, degrading only amino-terminal acidic amino acids from peptides. Therefore, attention is focused here on the efficient production this enzyme by recombinant Aspergillus oryzae and characterization its biochemical properties.The gene encoding DAP was overexpressed under taka-amylase promoter, with His-tag linker in A. oryzae, during cultivation Co(2+)-containing medium. The extracted mycelia purified immobilized nickel...