A5017088569- Plant Water Relations and Carbon Dynamics
- Plant responses to elevated CO2
- Fullerene Chemistry and Applications
- Graphene research and applications
- Spectroscopy and Quantum Chemical Studies
- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Advanced Chemical Physics Studies
- Protein Structure and Dynamics
- Inflammatory mediators and NSAID effects
- Advanced Chemical Sensor Technologies
- Plant Stress Responses and Tolerance
- DNA and Nucleic Acid Chemistry
- Forest ecology and management
- Boron and Carbon Nanomaterials Research
- Synthesis and Reactions of Organic Compounds
- Receptor Mechanisms and Signaling
- Ecology and Vegetation Dynamics Studies
- Photosynthetic Processes and Mechanisms
- Asthma and respiratory diseases
- Synthesis and biological activity
- Carbon Nanotubes in Composites
- Neuropeptides and Animal Physiology
- Enzyme Structure and Function
- Eicosanoids and Hypertension Pharmacology
Ritsumeikan University
1978-2025
Nagasaki University
2021-2025
Meiji University
2022-2024
Nihon University
2015-2024
The Institute of Statistical Mathematics
2023-2024
Ajinomoto (Japan)
2009-2023
National Institute of Advanced Industrial Science and Technology
2003-2023
Suzuki (Japan)
2020-2023
Omron (Japan)
2023
RIKEN Center for Computational Science
2016-2021
ATP has been shown to be a taste bud afferent transmitter, but the cells responsible for, and mechanism of, its release have not identified. Using CHO expressing high-affinity neurotransmitter receptors as biosensors, we show that gustatory stimuli cause receptor secrete through pannexin 1 hemichannels in mouse buds. further stimulates other second serotonin. These results provide link intracellular Ca(2+) during transduction secretion of ATP, from cells. They also indicate route for...
By human sensory analyses, we found that various extracellular calcium-sensing receptor (CaSR) agonists enhance sweet, salty, and umami tastes, although they have no taste themselves. These characteristics are known as "kokumi taste" often appear in traditional Japanese cuisine. Although GSH is a typical kokumi substance (taste enhancer), its mode of action poorly understood. Here, demonstrate how the enhanced by CaSR, close relative class C G-protein-coupled receptors T1R1, T1R2, T1R3...
We apply moving root-mean-square deviation (mRMSD), which does not require a reference structure, as method for analyzing protein dynamics. This can be used to calculate the (RMSD) of structure between two specified time points and analyze dynamics behavior through series analysis. applied this Trp-cage trajectory calculated by Anton supercomputer found that it shows regions stable states well conventional RMSD. In addition, we extracted characteristic in side chains Asp1 Arg16 form hydrogen...
A dissipative particle dynamics (DPD) simulation has been used to study the spontaneous vesicle formation of amphiphilic molecules in aqueous solution. The molecule is represented by a coarse-grained model, which contains hydrophilic head group and hydrophobic tail. Water also modeled same size as adopted amphiphile corresponding several H2O molecules. In DPD simulation, from both randomly dispersed system bilayer structure for initial condition, observed through intermediate state an oblate...
Taste buds are aggregates of 50–100 cells, only a fraction which express genes for taste receptors and intracellular signaling proteins. We combined functional calcium imaging with single-cell molecular profiling to demonstrate the existence two distinct cell types in mouse buds. Calcium revealed that isolated cells responded transient elevation cytoplasmic Ca 2+ either tastants or depolarization KCl, but never both. Using reverse transcription (RT)-PCR, we show individual phospholipase C β2...
The structure of ${\mathrm{C}}_{60}$ molecules adsorbed on the Cu(111)-(1\ifmmode\times\else\texttimes\fi{}1) surface has been investigated by scanning tunneling microscopy (STM). Bias voltage dependent STM images individual in monolayer film showed unique intramolecular structures with a threefold symmetry. observed agree well those calculated using local density approximation. With charge transfer from Cu(111) substrate to film, molecule ratchets hollow sites.
Taste buds contain two types of cells that directly participate in taste transduction - receptor (Type II) and presynaptic III) cells. Receptor respond to sweet, bitter umami stimulation but until recently the identity sour (acid) tastants has only been inferred from recordings situ, behavioural studies, immunostaining for putative molecules. Using calcium imaging on single isolated with biosensor identify neurotransmitter release, we show specifically acid release serotonin. By recording...
Synapses between gustatory receptor cells and primary sensory afferent fibers transmit the output signal from taste buds to CNS. Several transmitter candidates have been proposed for these synapses, including serotonin (5-HT), glutamate, acetylcholine, ATP, peptides, others, but, date, none has unambiguously identified. We used Chinese hamster ovary stably expressing 5-HT 2C receptors as biodetectors monitor release buds. When were depolarized with KCl or stimulated bitter, sweet, sour...
Recently, we reported that calcium-sensing receptor (CaSR) is a for kokumi substances, which enhance the intensities of salty, sweet and umami tastes. Furthermore, found several γ-glutamyl peptides, are CaSR agonists, substances. In this study, elucidated cells their physiological properties. For purpose, used Calcium Green-1 loaded mouse taste in lingual tissue slices confocal microscopy. Kokumi applied focally around pores, induced an increase intracellular Ca2+ concentration ([Ca2+]i)...
ABSTRACT The susceptibility to photoinhibition of tree species from three different successional stages were examined using chlorophyll fluorescence and gas exchange techniques. deciduous broadleaf Betula platyphylla var. japonica , pioneer early successional, Quercus mongolica intermediate shade‐tolerant mid‐successional, Acer mono late successional. Tree seedlings raised under light regimes: full sunlight (open), 10% sun, 5% sun. Susceptibility was assessed on the basis recovery kinetics...
Photosynthetic acclimation of deciduous broad-leaved tree species was studied along a vertical gradient within the canopy multi-species forest in northern Japan. We investigated variations (1) local light regime and CO2 concentration ([CO2]), (2) morphological (area, thickness area per mass), biochemical (nitrogen chlorophyll concentrations) physiological (light-saturated photosynthetic rate) attributes leaves seven major on three occasions (June, August October). early successional species,...
A number of gustatory receptors have been proposed to underlie umami, the taste L-glutamate, and certain other amino acids nucleotides. However, response profiles these cloned not validated against responses recorded from receptor cells that are native detectors umami taste. We investigated in mouse circumvallate buds an intact slice preparation, using confocal calcium imaging. Approximately 5% selectively responded L-glutamate when it was focally applied apical chemosensitive tips cells....
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSelective Ion-Binding by Protein Probed with the 3D-RISM TheoryNorio Yoshida, Saree Phongphanphanee, Yutaka Maruyama, Takashi Imai, and Fumio HirataView Author Information Department of Theoretical Molecular Science, Institute for Okazaki 444-8585, Japan, Functional The Graduate University Advanced Studies, Bioscience Bioinformatics, Ritsumeikan University, Kusatsu 525-8577, Japan Cite this: J. Am. Chem. Soc. 2006, 128, 37,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and properties of fluorine-containing aromatic polybenzoxazoles from bis(o-aminophenols) diacid chlorides by the silylation methodYutaka Maruyama, Yoshiyuki Oishi, Masaaki Kakimoto, Yoshio ImaiCite this: Macromolecules 1988, 21, 8, 2305–2309Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August...
The radioactive endohedral fullerenes ${}^{127}\mathrm{Xe}@{\mathrm{C}}_{60}$, ${}^{127}\mathrm{Xe}@{\mathrm{C}}_{70}$, ${}^{79}\mathrm{Kr}@{\mathrm{C}}_{60}$, and ${}^{79}\mathrm{Kr}@{\mathrm{C}}_{70}$, their dimers, are detected using radiochemical radiochromatographic techniques in the final solution of samples which Xe or Kr has penetrated into ${\mathrm{C}}_{60}$ ${\mathrm{C}}_{70}$ by a nuclear reaction recoil process. Carrying out ab initio molecular dynamics simulations based on an...
ATP and serotonin (5-HT) are neurotransmitters secreted from taste bud receptor (type II) presynaptic III) cells, respectively. Norepinephrine (NE) has also been proposed to be a neurotransmitter or paracrine hormone in buds. Yet, date, the specific stimulus for NE release buds is not well understood, identity of cells that secrete known. Chinese hamster ovary were transfected with alpha(1A) adrenoceptors loaded fura-2 ("biosensors") detect isolated mouse cells. Biosensors responded low...
Modulation of the calcium sensing receptor (CaSR) is one physiological activities γ-glutamyl peptides such as glutathione (γ-glutamylcysteinylglycine). γ-Glutamyl also possess a flavoring effect, i.e., sensory activity kokumi substances, which modifies five basic tastes when added to food. These have been shown be positively correlated, suggesting that are perceived through CaSRs in humans. Our research based on hypothesis discovery highly active CaSR agonist will lead creation practical...
Boron neutron capture therapy (BNCT) is a unique anticancer technology that has demonstrated its efficacy in numerous phase I/II clinical trials with boronophenylalanine (BPA) and sodium borocaptate (BSH) used as 10B delivery agents. However, continuous drug administration at high concentrations needed to maintain sufficient concentration within tumors. To address the issue of accumulation retention tumor tissue, we developed MMT1242, novel boron-containing α-d-mannopyranoside. We evaluated...
Collisions between ${\mathrm{C}}_{60}^{\phantom{\rule{0ex}{0ex}}\ensuremath{-}}$ and alkali-metal ion are investigated by an ab initio molecular dynamics simulation at 1000 K. When ${\mathrm{Li}}^{+}$ with kinetic energy $\ensuremath{\sim}5\mathrm{eV}$ hits the center of a six-membered ring ${\mathrm{C}}_{60}^{\phantom{\rule{0ex}{0ex}}\ensuremath{-}}$, Li@${\mathrm{C}}_{60}$ is created. If either lower or collision occurs off center, stays outside, ${\mathrm{C}}_{60}$ deformed shock. In some...
CO escaping pathways of Myoglobin were investigated in terms 3D distribution function which was calculated by the 3D-RISM theory. The partial molar volume changes through show excellent agreement with those from experiment.
RIKEN Center for Computational Science has been installing the supercomputer Fugaku. The Fujitsu A64FX, based on Armv8.2-A+SVE architecture, is used in system. In this paper, we evaluated seven HPC applications and benchmarks A64FX. a performance comparison with Marvell (Cavium) ThunderX2 processor Intel Xeon Skylake processor, A64FX achieved higher memory bandwidth-intensive application thanks to its high bandwidth. However, confirmed that of decreased from lack out-of-order resources. To...
Quantum coherence and its dephasing by coupling to a dissipative environment play an important role in time-resolved nonlinear optical response as well nonadiabatic transitions the condensed phase. We have discussed processes on multi-state one-dimensional system with Morse potential surfaces environment. This was based numerical study using quantum Fokker–Planck equation for colored Gaussian–Markovian noise bath, which expressed hierarchy of kinetic equations. can treat strong system-bath...