A5070777893- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromosomal and Genetic Variations
- Amphibian and Reptile Biology
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Genetic diversity and population structure
- Animal Behavior and Reproduction
- Escherichia coli research studies
- Education, Psychology, and Social Research
- Bacterial Genetics and Biotechnology
- Microbial Metabolism and Applications
- Monoclonal and Polyclonal Antibodies Research
- Celiac Disease Research and Management
- Infant Nutrition and Health
- Biochemical and Structural Characterization
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Burkholderia infections and melioidosis
- Lipid Membrane Structure and Behavior
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Transgenic Plants and Applications
- Cerebral Palsy and Movement Disorders
- Galectins and Cancer Biology
- Microbial Metabolites in Food Biotechnology
Czech Academy of Sciences, Institute of Molecular Genetics
2024
Gennet
2024
Charles University
2011-2021
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2011-2021
Masaryk University
2004-2019
Central European Institute of Technology
2019
Central European Institute of Technology – Masaryk University
2019
Czech Academy of Sciences
2010-2015
Genomics (United Kingdom)
2012
University of Cambridge
2011-2012
Squamate reptiles possess two general modes of sex determination: (1) genotypic determination (GSD), where the an individual is determined by chromosomes, i.e. sex-specific differences in genotype; and (2) temperature-dependent (TSD), chromosomes are absent nongenetic factors.After gathering information about sex-determining mechanisms for more than 400 species, we employed comparative phylogenetic analyses to reconstruct evolution Squamata.Our results suggest relative uniformity majority...
Many poikilothermic vertebrate lineages, especially among amphibians and fishes, possess a rapid turnover of sex chromosomes, while in endotherms there is notable stability chromosomes. Reptiles general exhibit variability sex-determining systems; as typical poikilotherms, they might be expected to have However, molecular data which would enable the testing chromosomes are lacking most lineages. Here, we provide evidence that highly conserved across iguanas, one species-rich clade reptiles....
One of the mechanisms contributing to protection by breast-feeding newborn against enteric diseases is related ability human milk oligosaccharides prevent attachment pathogenic bacteria duodenual epithelium. Indeed, a variety fucosylated oligosaccharides, specific milk, form part innate immune system. In present study, we demonstrate blocking PA-IIL, fucose-binding lectin pathogen Pseudomonas aeruginosa, oligosaccharides. Two epitopes, Lewis and 3-fucosyl-lactose (Lewis x glucose analogue)...
Abstract Background The accumulation of repetitive sequences such as microsatellites during the differentiation sex chromosomes has not been studied in most squamate reptiles (lizards, amphisbaenians and snakes), a group which large diversity determining systems. It is known that Bkm repeats containing tandem arrays GATA tetranucleotides are highly accumulated on degenerated W advanced snakes. Similar, potentially homologous, were found other vertebrates. Using FISH with probes all possible...
Monitor lizards are unique among ectothermic reptiles in that they have high aerobic capacity and distinctive cardiovascular physiology resembling of endothermic mammals. Here, we sequence the genome Komodo dragon Varanus komodoensis, largest extant monitor lizard, generate a high-resolution de novo chromosome-assigned assembly for V. komodoensis using hybrid approach long-range sequencing single-molecule optical mapping. Comparing with those related species, find evidence positive selection...
Vertebrates possess diverse sex-determining systems, which differ in evolutionary stability among particular groups. It has been suggested that poikilotherms more frequent turnovers of sex chromosomes than homoiotherms, whose effective thermoregulation can prevent the emergence reversals induced by environmental temperature. Squamate reptiles used to be regarded as a group with an extensive variability determination; however, we document how rather old radiation lizards from genus Anolis,...
Telomeres are nucleoprotein complexes protecting the physical ends of linear eukaryotic chromosomes and therefore helping to ensure their stability integrity. Additionally, telomeric sequences can be localized in non-terminal regions chromosomes, forming so-called interstitial (ITSs). ITSs traditionally considered relics chromosomal rearrangements thus very informative reconstruction evolutionary history karyotype formation. We examined distribution motifs (TTAGGG)n using fluorescence situ...
Sex chromosomes are believed to be stable in endotherms, but young and evolutionary unstable most ectothermic vertebrates. Within lacertids, the widely radiated lizard group, sex have been reported vary morphology heterochromatinization, which may suggest turnovers during evolution of group. We compared partial gene content Z-specific part across major lineages lacertids discovered a strong stability chromosomes. can conclude that common ancestor living around 70 million years ago (Mya),...
Abstract Amniotes possess variability in sex determination, ranging from environmental determination to genotypic with differentiated chromosomes. Differentiated chromosomes have emerged independently several times. Their noteworthy convergent characteristic is the evolutionary stability, documented among amniotes mammals, birds, and some lineages of lizards, snakes turtles. Combining analysis multiple partial transcriptomes comparison copy gene numbers between male female genomes, we...
Abstract PA‐IIL is a fucose‐binding lectin from Pseudomonas aeruginosa that closely related to the virulence factors of bacterium. Previous structural studies have revealed new carbohydrate‐binding mode with direct involvement two calcium ions (Mitchell E, Houles C, Sudakevitz D, Wimmerova M, Gautier Peréz S, Wu AM, Gilboa‐Garber N, Imberty A. Structural basis for selective recognition oligosaccharides cystic fibrosis patients by . Nat Struct Biol 2002;9:918–921). A combination...
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose D-arabinose that differ by the group at position 5 of sugar ring. Isothermal calorimetry experiments provided precise determination three methyl-glycosides revealed a large enthalpy contribution. crystal structures complexes PA-IIL with Met-beta-D-arabinoside have been determined compared...
Geckos (Gekkota) are a highly diversified group of lizards with an exceptional diversity in sex-determining systems. Despite this intriguing documented variability, data on sex determination many lineages is still scarce. Here, we document the previously overlooked heteromorphic ZZ/ZW chromosomes thick-tailed gecko, <i>Underwoodisaurus milii</i>, member ancient lineage pygopodoid geckos. The finding female heterogamety within geckos was unexpected, as until now only male and...
Abstract Amniotes possess variability in sex determining mechanisms, however, this diversity is still only partially known throughout the clade and systems remain unknown even such a popular distinctive lineage as chameleons (Squamata: Acrodonta: Chamaeleonidae). Here, we present evidence for female heterogamety group. The Malagasy giant chameleon ( Furcifer oustaleti ) (chromosome number 2n = 22) possesses heteromorphic Z W chromosomes with heterochromatic W. panther pardalis (2n 22 males,...
Iguanas (Pleurodonta) are predominantly distributed in the New World, but one previously cytogenetically understudied family, Opluridae, is endemic to Madagascar and adjacent Grand Comoro archipelago. The aim of our contribution fill a gap cytogenetic understanding this biogeographically puzzling lineage. Based on examination six species, we found that oplurids rather conservative karyotype, which composed 36 chromosomes as most iguanas. However, species differ position nucleolar organizer...
Snakes are historically important in the formulation of several central concepts on evolution sex chromosomes. For over 50 years, it was believed that all snakes shared same ZZ/ZW chromosomes, which homomorphic and poorly differentiated "basal" such as pythons boas, while heteromorphic well "advanced" (caenophidian) snakes. Recent molecular studies revealed chromosomes indeed among families caenophidian snakes, but boas evolved likely independently male heterogamety (XX/XY chromosomes). The...
The green anole, Anolis carolinensis (ACA), is the model reptile for a vast array of biological disciplines. It was first nonavian to have its genome fully sequenced. During project, XX/XY system sex chromosomes homologous chicken chromosome 15 (GGA15) revealed, and 106 X-linked genes were identified. We selected 38 located on eight scaffolds in ACA having orthologs GGA15, then tested their linkage X by using comparative quantitative fluorescent real-time polymerase chain reaction applied...