A5021155049- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Advanced Memory and Neural Computing
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Cell Image Analysis Techniques
University of Science and Technology of China
2018-2023
Stanford University
2018-2021
Center for Excellence in Brain Science and Intelligence Technology
2018
ABSTRACT Imaging of transmembrane voltage deep in brain tissue with cellular resolution has the potential to reveal information processing by neuronal circuits living animals minimal perturbation. Multi-photon imaging vivo , however, is currently limited speed and sensitivity both indicators methods. Here, we report engineering an improved genetically encoded indicator, ASAP3, which exhibits up 51% fluorescence responses physiological range, sub-millisecond activation kinetics, full...
Abstract Neuronal spiking activity is routinely recorded using genetically encoded calcium indicators (GECIs), but imaging limited in temporal resolution and does not report subthreshold voltage changes. Genetically (GEVIs) offer better sensitivity, spike detection with fast GEVIs has required specialized equipment. Here, we the ASAP4 subfamily of that brighten response to membrane depolarization, inverting fluorescence-voltage relationship previous ASAP GEVIs. Two variants, ASAP4b ASAP4e,...
Recent interest in high-throughput recording of neuronal activity has motivated rapid improvements genetically encoded calcium or voltage indicators (GECIs GEVIs) for all-optical electrophysiology. Among these probes, the ASAPs, a series based on variant circularly permuted green fluorescent protein (cpGFP) and conjugated sensitive domain (VSD), are capable detecting both action potentials subthreshold activities. Here we show that when excited by blue light, undergo reversible...
Acetylcholine (ACh) is an important neuromodulator in various cognitive functions. However, it unclear how ACh influences neural circuit dynamics by altering cellular properties. Here, we investigated reverberatory activity cultured neuronal networks. We found that suppressed the occurrence of evoked reverberation at low to moderate doses, but a much lesser extent high doses. Moreover, doses caused longer duration reverberation, and higher spontaneous activity. With whole-cell recording from...
Abstract Detection of somatosensory inputs requires conversion external stimuli into electrical signals by activation primary sensory neurons. The mechanisms which heterogeneous neurons encode different remains unclear. In vivo dorsal root ganglia (DRG) imaging using genetically-encoded Ca 2+ indicators (GECIs) is currently the best technique for this purpose providing an unprecedented spatial and populational resolution. It permits simultaneous >1800 neurons/DRG in live mice. However,...
Technologies for recording and manipulating transmembrane potential in vivo defined neuronal populations with high fidelity will be essential to understand how information is represented, processed, propagated the brain. Genetically encoded voltage indicators (GEVIs) optogenetic actuators are especially promising as they can expressed cell types compatible long-term chronic imaging vivo. Cellular vivo, however, suffers from limitations of both speed sensitivity inherent current modalities.