A5084839529- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neuropharmacology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- S100 Proteins and Annexins
- Advanced Biosensing Techniques and Applications
- CCD and CMOS Imaging Sensors
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurological Disease Mechanisms and Treatments
- Cell Image Analysis Techniques
- Photoreceptor and optogenetics research
- Acute Ischemic Stroke Management
- Neuroscience and Neural Engineering
- Olfactory and Sensory Function Studies
University of California, Los Angeles
2020-2025
University of Tennessee Health Science Center
2020
Working memory (WM) and timing are considered distinct cognitive functions, yet the neural signatures underlying both can be similar. To address hypothesis that WM may multiplexed we developed a novel rodent task where 1st odor identity predicts delay duration. We found performance decreased when expectations were violated. Performance was worse for unexpected long delays than short delays, suggesting tuned to expire in delay-dependent manner. Calcium imaging of dorsal CA1 neurons revealed...
Here we describe the development of UCLA 2P Miniscope, an easily adopted, open-source miniature 2-photon microscope capable recording calcium dynamics from neurons located in deep structures and dendrites over a 445 μm x 380 field view (FOV) during free behavior. The system weighs approximately 4g utilizes two on-board silicon-based photon detectors for highly sensitive measurements. All hardware is designed high performance ease assembly, while minimizing cost. To test microscope, recorded...
Abstract There is considerable concern about the long‐term deleterious effects of repeat head trauma on cognition, but little known underlying mechanisms and pathology. To examine this, we delivered four air blasts to left side mouse cranium, a week apart, with an intensity that causes deficits when singly considered “concussive,” or does not yield significant “subconcussive.” Neither concussive nor subconcussive blast produced spatial memory at 4 months, both yielded 14 dorsal hippocampal...
Application of compressed imaging methods in optics has led to advancements high-speed snapshot such as Light Field Tomography (LIFT). LIFT utilizes computed tomography and light field compress a 3-D view into 1-D line light. It can achieve excellent speeds when used with linear sensor. We propose using image multi-neuron dynamics the ASAP voltage indicator, which proven challenging due millisecond low brightness. Here we report prototype system confirm performance by high speed GCaMP...
ABSTRACT Behavioral timescale synaptic plasticity (BTSP) is a form of potentiation where the occurrence single large plateau potential in CA1 hippocampal neurons leads to formation reliable place fields during spatial learning tasks. We asked whether BTSP could also be mechanism for generation non-spatial responses hippocampus and what roles medial lateral entorhinal cortex (MEC LEC) play driving BTSP. By performing simultaneous calcium imaging dorsal chemogenetic inhibition LEC or MEC while...