A5063153560- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Retinal Development and Disorders
- Neuroscience and Neural Engineering
- Receptor Mechanisms and Signaling
- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- Insect-Plant Interactions and Control
- RNA Research and Splicing
- Online and Blended Learning
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Retinoids in leukemia and cellular processes
- Memory and Neural Mechanisms
- Forest Insect Ecology and Management
- MicroRNA in disease regulation
- Advanced Memory and Neural Computing
- Medicinal Plants and Neuroprotection
- Insect and Pesticide Research
- Gender and Technology in Education
- Cardiac electrophysiology and arrhythmias
Columbia University
2021-2023
New York Proton Center
2021
Stanford University
2020
The University of Texas Southwestern Medical Center
2012-2017
Southwestern Medical Center
2013-2016
University of Michigan
2010
Another micropeptide flexes its muscle Genome annotation is a complex but imperfect art. Attesting to limitations the growing evidence that certain transcripts annotated as long noncoding RNAs (lncRNAs) in fact code for small peptides with biologically important functions. One such lncRNA-derived mammals myoregulin, which reduces performance by inhibiting activity of key calcium pump. Nelson et al. describe opposite second mammalian muscle, called DWORF (see Perspective Payre and Desplan)....
Homeostatic scaling allows neurons to maintain stable activity patterns by globally altering their synaptic strength in response changing levels. Suppression of the blocking action potentials increases through an upregulation surface α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. Although this upscaling was shown require transcription, molecular nature intrinsic transcription program underlying process and its functional significance have been unclear. Using RNA-seq,...
Spontaneous glutamate release-driven NMDA receptor activity exerts a strong influence on synaptic homeostasis. However, the properties of Ca2+ signals that mediate this effect remain unclear. Here, using hippocampal neurons labeled with fluorescent probes Fluo-4 or GCAMP5, we visualized action potential-independent transients in dendritic regions adjacent to fluorescently presynaptic boutons physiological levels extracellular Mg2+. These required activity, and their propensity correlated...
Recent studies indicate that within individual synapses spontaneous and evoked release processes are segregated regulated independently. In the hippocampus, earlier electrophysiological recordings suggested glutamate can activate separate groups of postsynaptic NMDA receptors with limited overlap. However, it is still unclear how this separation distributed across synapses. a previous paper (<xref ref-type="bibr" rid="bib14">Reese Kavalali, 2015</xref>) we showed receptor...
Abstract Neuronal spiking activity is routinely recorded using genetically encoded calcium indicators (GECIs), but imaging limited in temporal resolution and does not report subthreshold voltage changes. Genetically (GEVIs) offer better sensitivity, spike detection with fast GEVIs has required specialized equipment. Here, we the ASAP4 subfamily of that brighten response to membrane depolarization, inverting fluorescence-voltage relationship previous ASAP GEVIs. Two variants, ASAP4b ASAP4e,...
Significance The CLC-2 ion channel facilitates selective passage of Cl – ions across cell membranes. In the central nervous system, is expressed in both neurons and glia proposed to regulate electrical excitability homeostasis. has been implicated various system disorders, including certain types epilepsy leukodystrophy. Establishing a causative role for neuropathologies, however, limited by absence reagents that enable acute specific modulation. Our studies have resulted identification...
Background: Mammalian transcriptomes contain thousands of potential open reading frames (ORFs) with fewer than fifty codons. Most these ORFs are presumed to be random and therefore noncoding, but recently several genes annotated as long noncoding RNAs (lncRNAs) were shown encode functional micropeptides. Objective: Identify characterize novel micropeptides encoded in cardiac-expressed lncRNAs using comparative genomics. Methods Results: Using codon substitution frequency, a genomics...
Abstract CLC-2 is a voltage-gated chloride channel that widely expressed in many mammalian tissues. In the central nervous system (CNS), neurons and glia. Studies to define how this contributes normal pathophysiological function CNS have been controversial, part due absence of precise pharmacological tools for modulating activity. Herein, we describe development optimization AK-42, specific small-molecule inhibitor with nanomolar potency (IC 50 = 17 ± 1 nM). AK-42 displays unprecedented...
Background: Our lab has discovered a novel conserved micropeptide of 34 amino acids encoded by muscle-specific RNA that was previously annotated as putative long non-coding (lncRNA). This micropeptide, which we named DWORF (DWarf Open Reading Frame), shares structural similarity with the known SERCA modulators phospholamban (PLN), sarcolipin (SLN) and myoregulin (MLN). Objective: To define functional role in heart elucidate its mechanism action. Methods Results: Immunofluorescence staining...