A5004750602- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Biochemical Analysis and Sensing Techniques
- Receptor Mechanisms and Signaling
- Viral Infections and Immunology Research
- Neuroscience and Neuropharmacology Research
- Viral gastroenteritis research and epidemiology
- Vestibular and auditory disorders
- Neuroscience of respiration and sleep
- Spaceflight effects on biology
- Nitric Oxide and Endothelin Effects
- Cerebrospinal fluid and hydrocephalus
- Cholinesterase and Neurodegenerative Diseases
- bioluminescence and chemiluminescence research
- Estrogen and related hormone effects
- Physiological and biochemical adaptations
- Reproductive Biology and Fertility
- Ion channel regulation and function
- Molecular Sensors and Ion Detection
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
Inserm
2018-2024
Centre National de la Recherche Scientifique
2009-2024
Institut de Biologie de l'École Normale Supérieure
2018-2024
Université Paris Sciences et Lettres
2018-2024
École Normale Supérieure - PSL
2018-2024
Université Paris Cité
2005-2020
SPPIN - Saints-Pères Paris Institute for Neurosciences
2020
Institut de Psychiatrie et Neurosciences de Paris
2020
Brain Physiology Lab
2013-2015
Délégation Paris 5
2009-2013
Plasmids containing the entire cDNA sequence of poliovirus type 1 (Mahoney strain) under control a promoter for T7 RNA polymerase have been constructed. Purified efficiently transcribes in either direction to produce full-length [(+)RNA] or its complement [(-)RNA]. The (+)RNA produced initially had 60 nucleotides on 5' side sequence, including string 18 consecutive guanine residues generated original cloning and an additional 626 pBR322 beyond poly(A) tract at 3' end. Such RNA, while much...
Genetically encoded voltage indicators are emerging tools for monitoring dynamics with cell-type specificity. However, current enable a narrow range of applications due to poor performance under two-photon microscopy, method choice deep-tissue recording. To improve indicators, we developed multiparameter high-throughput platform optimize microscopy. Using this system, identified JEDI-2P, an indicator that is faster, brighter, and more sensitive photostable than its predecessors. We...
Olfactory receptor neurons respond to odorant stimulation with a rapid increase in intracellular cAMP that opens cyclic nucleotide-gated (cng) cation channels. cng channels rat olfactory are activated by the low micromolar range and outwardly rectifying. The cloned channel (rOCNC1), however, is much less sensitive exhibits very weak rectification. Here we describe cloning characterization of second subunit, denoted rOCNC2. rOCNC2 does not form functional when expressed alone. When rOCNC1...
Cyclic nucleotide-gated (CNG) channels play central roles in visual and olfactory signal transduction. In the retina, rod photoreceptors express subunits CNCα1 CNCβ1a. cone photoreceptors, only CNCα2 expression has been demonstrated so far. Rat sensory neurons (OSNs) two homologous subunits, here designated CNCα3 CNCα4. This paper describes characterization of CNCβ1b, a third subunit expressed OSNs establishes it as component native channel. CNCβ1b is an alternate splice form photoreceptor...
The mouse-adapted strain of poliovirus type 2 (Lansing) induces fatal poliomyelitis in mice after intracerebral inoculation, whereas inoculated with 1 (Mahoney) show no signs disease. Previous work indicated that the adaptation to mouse virulence is associated viral capsid proteins and mutations neutralization antigenic site I reduce neurovirulence Lansing mice. role was further explored by constructing an hybrid virus. Six amino acids Mahoney were replaced a sequence specific for using...
Cyclic nucleotide-gated (cng) channels are important components of signaling systems mediating sensory transduction. In vertebrate photoreceptors, light activates a cascade that causes decrease in intracellular cGMP concentrations, closing retinal cng channels. Signal transduction olfactory receptor neurons is believed to proceed via G-protein-mediated elevation cAMP response odorant binding by 7-helix receptors. opens the channel, which highly permeable Ca 2+ . Here we demonstrate situ...
A major goal in neuroscience is the development of optical reporters membrane potential that are easy to use, have limited phototoxicity, and achieve speed sensitivity necessary for detection individual action potentials single neurons. Here we present a novel, two-component approach attains these goals. By combining DiO, fluorescent neuronal tracer dye, with dipicrylamine (DPA), molecule whose partitioning voltage-sensitive, signals related changes based on FRET (Förster resonance energy...
The advent of scanning two-photon microscopy (2PM) has created a fertile new avenue for noninvasive investigation brain activity in depth. One principal weakness this method, however, lies with the limit speed, which makes optical interrogation action potential-like neuronal network problematic. Encoded multisite (eMS2PM), scanless method that allows simultaneous imaging multiple targets depth high temporal resolution, addresses drawback. eMS2PM uses liquid crystal spatial light modulator to...
The nematode Caenorhabditis elegans exhibits behavioral responses to many volatile odorants. Chemotaxis toward one such odorant, diacetyl (butanedione), requires the function of a seven-transmembrane receptor protein encoded by odr-10 gene. To determine directly whether ODR-10 is an odorant receptor, it necessary express in heterologous system and show that responds activation G signaling pathway. Here we demonstrate human cells expressing on their surfaces exhibit transient elevation...
Calcium (Ca 2+ ) influx through Ca -permeable ion channels plays a pivotal role in variety of neuronal signaling processes, and negative-feedback control this by itself is often equally important for modulation such signaling. Negative calmodulin (CaM) on cyclic nucleotide–gated (CNG) underlies the adaptation olfactory receptor neurons to odorants. We show that feedback requires two additional subunits native channel, CNGA4 CNGB1b, even though machinery CaM binding present principal subunit...
Abstract Faithful reporting of temporal patterns intracellular Ca 2+ dynamics requires the working range indicators to match signals. Current genetically encoded calmodulin-based fluorescent are likely distort fast signals by apparent saturation and integration due their limiting fluorescence rise decay kinetics. A series probes was engineered with a affinities accelerated kinetics weakening -calmodulin-peptide interactions. At 37 °C, GCaMP3-derived probe termed GCaMP3 is 40-fold faster than...
Olfactory receptor neurons (ORNs) employ a cyclic nucleotide-gated (CNG) channel to generate current in response an odorant-induced rise cAMP. This contains three types of subunits, the principal CNGA2 subunit and two modulatory subunits (CNGA4 CNGB1b). Here, we have analyzed functional relevance CNGB1 for olfaction by gene targeting mice. Electro-olfactogram responses CNGB1-deficient (CNGB1-/-) mice displayed reduced maximal amplitude decelerated onset recovery kinetics compared with...
The maturation of the poliovirus capsid occurs as result a single unexplained proteolytic event during which 58 to 59 copies 60 VP0 protein precursors are cleaved. An autocatalytic mechanism for cleavage VP4 and VP2 was proposed by Arnold et al. (E. Arnold, M. Luo, G. Vriend, Rossman, A. C. Palmenberg, D. Parks, J. Nicklin, E. Wimmer, Proc. Natl. Acad. Sci. USA 84:21-25, 1987) in serine 10 is activated virion RNA catalyze VP4-VP2 processing. hypothesis rests on observation that hydrogen bond...
While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in mammalian brain, much remains to learned concerning circumstances and cellular mechanisms governing potential action. Using a combination of optogenetics two-photon calcium imaging vivo, we find activation chloride-permeable GABAA receptors parallel fibers (PFs) cerebellar molecular layer adult mice causes fiber excitation. Stimulation PFs at submaximal stimulus intensities leads release...