A5081505476- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Neural dynamics and brain function
- Animal Behavior and Reproduction
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Plant and Biological Electrophysiology Studies
- Circadian rhythm and melatonin
- Ion channel regulation and function
- Physiological and biochemical adaptations
- Morphological variations and asymmetry
- Insect and Pesticide Research
- Ion Channels and Receptors
- Neuroscience and Neural Engineering
- Real-time simulation and control systems
- Plant and animal studies
- Lipid Membrane Structure and Behavior
- Electrochemical Analysis and Applications
- Vestibular and auditory disorders
- Advanced Fluorescence Microscopy Techniques
- Insect Utilization and Effects
- Memory and Neural Mechanisms
- Retinal Development and Disorders
- Bioinformatics and Genomic Networks
- Neural Networks and Applications
Janelia Research Campus
2015-2024
Howard Hughes Medical Institute
2015-2021
Université Paris Cité
2009-2015
Brain Physiology Lab
2011-2015
Centre National de la Recherche Scientifique
2003-2015
Délégation Paris 5
2009-2013
Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron microscopy-based connectome of
Many animals maintain an internal representation of their heading as they move through surroundings. Such a compass was recently discovered in neural population the Drosophila melanogaster central complex, brain region implicated spatial navigation. Here, we use two-photon calcium imaging and electrophysiology head-fixed walking flies to identify different that conjunctively encodes angular velocity, is excited selectively by turns either clockwise or counterclockwise direction. We show how...
Animals perform many stereotyped movements, but how nervous systems are organized for controlling specific movements remains unclear. Here we use anatomical, optogenetic, behavioral, and physiological techniques to identify a circuit in Drosophila melanogaster that can elicit leg groom the antennae. Mechanosensory chordotonal neurons detect displacements of antennae excite three different classes functionally connected interneurons, which include two brain interneurons parallel descending...
The central complex is a highly conserved insect brain region composed of morphologically stereotyped neurons that arborize in distinctively shaped substructures. implicated wide range behaviors and several modeling studies have explored its circuit computations. Most relied on assumptions about connectivity between based their overlap light microscopy images. Here, we present an extensive functional connectome Drosophila melanogaster's at cell-type resolution. Using simultaneous optogenetic...
Animals use acoustic signals across a variety of social behaviors, particularly courtship. In Drosophila, song is detected by antennal mechanosensory neurons and further processed second-order aPN1/aLN(al) neurons. However, little known about the central pathways mediating courtship hearing. this study, we identified male-specific pathway for hearing via third-order ventrolateral protocerebrum Projection Neuron 1 (vPN1) fourth-order pC1 Genetic inactivation vPN1 or disrupts song-induced...
ABSTRACT Flexible behaviors over long timescales are thought to engage recurrent neural networks in deep brain regions, which experimentally challenging study. In insects, circuit dynamics a region called the central complex (CX) enable directed locomotion, sleep, and context- experience-dependent spatial navigation. We describe first complete electron-microscopy-based connectome of Drosophila CX, including all its neurons circuits at synaptic resolution. identified new CX neuron types,...
Cell-attached recording is extensively used to study the firing rate of mammalian neurons, but potential limitations method have not been investigated in detail. Here we perform cell-attached molecular layer interneurons cerebellar slices from rats and mice, how experimental conditions influence measured rate. We find that this depends on time mode, pipette potential, ionic composition. In first minute after sealing, action currents are variable shape size, presumably reflecting membrane...
Abstract The forthcoming assembly of the adult Drosophila melanogaster central brain connectome, containing over 125,000 neurons and 50 million synaptic connections, provides a template for examining sensory processing throughout brain. Here, we create leaky integrate-and-fire computational model entire brain, based on neural connectivity neurotransmitter identity, to study circuit properties feeding grooming behaviors. We show that activation sugar-sensing or water-sensing gustatory in...
While it has been proposed that the conventional inhibitory neurotransmitter GABA can be excitatory in mammalian brain, much remains to learned concerning circumstances and cellular mechanisms governing potential action. Using a combination of optogenetics two-photon calcium imaging vivo, we find activation chloride-permeable GABAA receptors parallel fibers (PFs) cerebellar molecular layer adult mice causes fiber excitation. Stimulation PFs at submaximal stimulus intensities leads release...
We examined the relationship between somatic Ca 2+ signals and spiking activity of cerebellar molecular layer interneurons (MLIs) in adult mice. Using two-photon microscopy conjunction with cell-attached recordings slices, we show that tonically firing MLIs loaded high-affinity probes, -dependent fluorescence transients are absent. Spike-triggered averages traces for at low rates revealed change associated an action potential is small (1% basal fluorescence). To uncover intracellular...
Little is known about the generation of slow rhythms in brain neuronal circuits. Nevertheless, a few studies, both from reconstituted systems and hippocampal slices, indicate that activation metabotropic glutamate receptors (mGluRs) could generate such rhythms. Here we show rat cerebellar slices after either release by repetitive stimulation, or direct stimulation type 1 mGluRs, molecular layer interneurons exhibit Ca 2+ transients. By combining cell-attached patch-clamp recording with...
The excitatory postsynaptic currents (EPSCs) evoked in Purkinje cells (PCs) by stimulating parallel fibres (PFs) usually show a single peak, but EPSCs with multiple peaks (polyphasic EPSCs) can be observed slices from animals older than 15 days. remain polyphasic when the current is reduced (either reducing intensity of PF stimulation or adding AMPA receptor antagonists) and PC membrane potential made positive. Thus late are not due to active generated imperfectly clamped PC, must arise...
Abstract Diverse subpopulations of mechanosensory neurons detect different mechanical forces and influence behavior. How these connect with central circuits to behavior remains an important area study. We previously discovered a neural circuit that elicits grooming the Drosophila melanogaster antennae is activated by antennal chordotonal organ, Johnston’s organ (JO) (Hampel et al., 2015). Here, we describe anatomically physiologically distinct JO neuron define how they interface grooming....