A5043474639- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Plant Molecular Biology Research
- Insect Utilization and Effects
- Cell Image Analysis Techniques
- Physiological and biochemical adaptations
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- Viral Infectious Diseases and Gene Expression in Insects
- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Advanced Fluorescence Microscopy Techniques
- Zebrafish Biomedical Research Applications
- CRISPR and Genetic Engineering
- Genetics and Neurodevelopmental Disorders
- Axon Guidance and Neuronal Signaling
- Multisensory perception and integration
- Genomics and Chromatin Dynamics
- Insect symbiosis and bacterial influences
- Protein Degradation and Inhibitors
- Digital Imaging for Blood Diseases
- Signaling Pathways in Disease
- Plant responses to water stress
- Insect behavior and control techniques
Howard Hughes Medical Institute
2021-2025
Janelia Research Campus
2021-2025
University of California, Berkeley
2021-2025
University of Rochester
2025
University of Rochester Medical Center
2023-2024
University of Cologne
2024
The University of Queensland
2024
Columbia University
2024
MRC Laboratory of Molecular Biology
2023
University of Michigan–Ann Arbor
2013-2023
Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of
Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we...
Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
Taste detection and hunger state dynamically regulate the decision to initiate feeding. To study how context-appropriate feeding decisions are generated, we combined synaptic resolution circuit reconstruction with targeted genetic access specific neurons elucidate a gustatory sensorimotor for initiation in adult Drosophila melanogaster. This connects sensory proboscis motor through three intermediate layers. Most this pathway necessary sufficient extension, behavior, respond selectively...
Gustatory sensory neurons detect caloric and harmful compounds in potential food convey this information to the brain inform feeding decisions. To examine signals that gustatory transmit receive, we reconstructed axons their synaptic sites adult
Mated females reallocate resources to offspring production, causing changes nutritional requirements and challenges energy homeostasis. Although observed across species, the neural endocrine mechanisms that regulate needs of mated are not well understood. Here, we find
The mushroom body (MB) is the center for associative learning in insects. In Drosophila, intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending...
The mushroom body (MB) is the center for associative learning in insects. In Drosophila, intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images...
The mushroom body (MB) is the center for associative learning in insects. In Drosophila , intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
The mushroom body (MB) is the center for associative learning in insects. In Drosophila , intersectional split-GAL4 drivers and electron microscopy (EM) connectomes have laid foundation precise interrogation of MB neural circuits. However, investigation many cell types upstream downstream has been hindered due to lack specific driver lines. Here we describe a new collection over 800 split-LexA that cover approximately 300 types, including sugar sensory neurons, putative nociceptive ascending...
Pattern recognition algorithms are useful in bioimage informatics applications such as quantifying cellular and subcellular objects, annotating gene expressions, classifying phenotypes. To provide effective efficient image classification annotation for the ever-increasing microscopic images, it is desirable to have tools that can combine compare various algorithms, build customizable solution different biological problems. However, current often offer a limited generating user-friendly...
Abstract The forthcoming assembly of the adult Drosophila melanogaster central brain connectome, containing over 125,000 neurons and 50 million synaptic connections, provides a template for examining sensory processing throughout brain. Here, we create leaky integrate-and-fire computational model entire brain, based on neural connectivity neurotransmitter identity, to study circuit properties feeding grooming behaviors. We show that activation sugar-sensing or water-sensing gustatory in...
Increased expression of Down Syndrome Cell Adhesion Molecule (Dscam) is implicated in the pathogenesis brain disorders such as syndrome (DS) and fragile X (FXS). Here, we show that cellular defects caused by dysregulated Dscam levels can be ameliorated genetic pharmacological inhibition Abelson kinase (Abl) both Dscam-overexpressing neurons a Drosophila model syndrome. This study offers Abl potential therapeutic target for treating associated with expression.
Abstract Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting typically documented using light microscopy full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here we use stochastic labeling with MultiColor FlpOut approach generate cellular confocal images at large scale. We are releasing aligned 74,000 such adult...
Abstract Walking is a complex motor program involving coordinated and distributed activity across the brain spinal cord. Halting appropriately at correct time critical but often overlooked component of walking control. While recent studies have delineated specific genetically defined neuronal populations in mouse brainstem that drive different types halting 1–3 , underlying neural circuit mechanisms responsible for overruling competing walking-state to generate context-appropriate halting,...
Neurons typically assume multipolar, bipolar, or unipolar morphologies. Little is known about the mechanisms underlying development of these basic morphological types. Here, we show that Krüppel-like transcription factor Dar1 determines multipolar morphology postmitotic neurons in Drosophila . specifically expressed and loss dar1 gradually converts into bipolar without changing neuronal identity. Conversely, misexpression its mammalian homolog causes them to regulates expression several...