A5058175232- Neuroendocrine regulation and behavior
- Neurobiology and Insect Physiology Research
- Stress Responses and Cortisol
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Child and Adolescent Psychosocial and Emotional Development
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Image Analysis Techniques
- Insect and Arachnid Ecology and Behavior
- Plant Molecular Biology Research
- Pluripotent Stem Cells Research
- Neuropeptides and Animal Physiology
- Developmental Biology and Gene Regulation
- Protein Degradation and Inhibitors
- Tryptophan and brain disorders
- Biochemical Analysis and Sensing Techniques
- Animal Genetics and Reproduction
- Receptor Mechanisms and Signaling
- Click Chemistry and Applications
- Ubiquitin and proteasome pathways
- Regulation of Appetite and Obesity
- Bipolar Disorder and Treatment
- Treatment of Major Depression
- Plant biochemistry and biosynthesis
- Genetic Neurodegenerative Diseases
Janelia Research Campus
2018-2025
Howard Hughes Medical Institute
2018-2025
University of Cologne
2024-2025
Columbia University
2024
The University of Queensland
2024
University of California, Berkeley
2024
MRC Laboratory of Molecular Biology
2018
Medical Research Council
2018
National Institute of Mental Health
2008-2017
National Institutes of Health
2008-2017
Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of
The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping individual neurons and neural subtypes. Brain templates are essential comparative biological studies because they analyzing many individuals in common reference space. Several central brain exist Drosophila, but every one either biased, uses sub-optimal tissue preparation, imaged at low resolution, or does not account artifacts. No publicly...
Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
Research suggests that dysfunctional glutamatergic signalling may contribute to depression, a debilitating mood disorder affecting millions of individuals worldwide. Ketamine, N-methyl-d-aspartate (NMDA) receptor antagonist, exerts rapid antidepressant effects in approximately 70% patients. Glutamate evokes the release d-serine from astrocytes and neurons, which then acts as co-agonist binds at glycine site on NR1 subunit NMDA receptors. Several studies have implicated glial deficits one...
Numerous genetic and functional studies implicate variants of Neuregulin-1 (NRG1) its neuronal receptor ErbB4 in schizophrenia many endophenotypes. Although the neurophysiological behavioral phenotypes NRG1 mutant mice have been investigated extensively, practically nothing is known about function NRG2, closest homolog. We found that NRG2 expression adult rodent brain does not overlap with more extensive than originally reported, including striatum medial prefrontal cortex (mPFC), therefore...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
Identifying the neurotransmitters used by specific neurons is a critical step in understanding function of neural circuits. However, methods for consistent and efficient detection neurotransmitter markers remain limited. Fluorescence situ hybridization (FISH) enables direct labeling type-specific mRNA neurons. Recent advances FISH allow this technique to be carried out intact tissue samples such as whole-mount Drosophila melanogaster brains. Here, we present platform high-throughput eight...
Abstract Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting typically documented using light microscopy full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here we use stochastic labeling with MultiColor FlpOut approach generate cellular confocal images at large scale. We are releasing aligned 74,000 such adult...
The use of genetically encoded 'self-labeling tags' with chemical fluorophore ligands enables rapid labeling specific cells in neural tissue. To improve the tagging neurons, we synthesized and evaluated new based on Cy, Janelia Fluor, Alexa ATTO dyes tested these recently improved Drosophila melanogaster transgenes. We found that tissue clearing mounting DPX substantially improves signal quality when combined non-cyanine fluorophores. compared this technique standard immunohistochemistry brain.
Abstract The fruit fly Drosophila melanogaster is an important model organism for neuroscience with a wide array of genetic tools that enable the mapping individuals neurons and neural subtypes. Brain templates are essential comparative biological studies because they analyzing many in common reference space. Several central brain exist , but every one either biased, uses sub-optimal tissue preparation, imaged at low resolution, or does not account artifacts. No publicly available ventral...