Christopher T Zugates
A5069898332- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Plant Molecular Biology Research
- Advanced Electron Microscopy Techniques and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Fluorescence Microscopy Techniques
- Insect and Arachnid Ecology and Behavior
- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Medical Imaging Techniques and Applications
- Integrated Circuits and Semiconductor Failure Analysis
- Ga2O3 and related materials
- Anodic Oxide Films and Nanostructures
- Engineering Applied Research
- Plant biochemistry and biosynthesis
- Thermography and Photoacoustic Techniques
- Genetics, Aging, and Longevity in Model Organisms
- Protein Degradation and Inhibitors
- Radiation Detection and Scintillator Technologies
- GaN-based semiconductor devices and materials
- TGF-β signaling in diseases
- Axon Guidance and Neuronal Signaling
- Advanced X-ray and CT Imaging
- Invertebrate Immune Response Mechanisms
- Radiomics and Machine Learning in Medical Imaging
University of Cologne
2024-2025
Howard Hughes Medical Institute
2012-2025
Janelia Research Campus
2012-2025
The University of Queensland
2024
University of California, Berkeley
2024
Columbia University
2024
Helix (United States)
2012
University of Illinois Urbana-Champaign
2002-2006
ChemImage (United States)
2000-2001
Los Alamos National Laboratory
2000
We established a collection of 7,000 transgenic lines Drosophila melanogaster. Expression GAL4 in each line is controlled by different, defined fragment genomic DNA that serves as transcriptional enhancer. used confocal microscopy dissected nervous systems to determine the expression patterns driven adult brain and ventral nerve cord. present image data on 6,650 lines. Using both manual machine-assisted annotation, we describe most useful illustrate utility these for identifying novel...
Optical and electron microscopy have made tremendous inroads toward understanding the complexity of brain. However, optical offers insufficient resolution to reveal subcellular details, lacks throughput molecular contrast visualize specific constituents over millimeter-scale or larger dimensions. We combined expansion lattice light-sheet image nanoscale spatial relationships between proteins across thickness mouse cortex entire Drosophila These included synaptic at dendritic spines,...
Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of
Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images...
Abstract Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting typically documented using light microscopy full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here we use stochastic labeling with MultiColor FlpOut approach generate cellular confocal images at large scale. We are releasing aligned 74,000 such adult...
Raman chemical imaging and scanning electron microscopy (Raman/SEM) have been used in a preliminary study to determine the size, morphology, elemental molecular composition, structure of fine particulate matter several test samples one ambient air sample. SEM, respectively, provide way spatially characterize sample based on its makeup. When combined, SEM detailed spatial, elemental, information for as small 250 nm. Initial studies demonstrate potential Raman/SEM determination organic...
Electron irradiation during reflection high-energy electron diffraction is shown to affect the materials properties of ZnSe and GaN growth by molecular beam epitaxy. The electrons produce an stimulated desorption effect ZnSe, which primarily affects adsorbed Se. Se rates under are be significantly larger than thermal rates. also decreases Zn-rich conditions. decrease can suppressed either Se-rich conditions or using high index substrate orientations, in this case (211)B. influences for grown...
An integrated analytical approach, which combines elemental [scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS)] and molecular (Raman) chemical imaging techniques, has been utilized for the characterization of a residual phase gallium in mixed oxide (MOX) surrogate material. Surrogate MOX materials are comprised CeO 2 place PuO . In real materials, is UO as commercial fuel source. A Ga remains sample following reductive heating experiments designed to explore...
Abstract Optical and electron microscopy have made tremendous inroads in understanding the complexity of brain, but former offers insufficient resolution to reveal subcellular details latter lacks throughput molecular contrast visualize specific constituents over mm-scale or larger dimensions. We combined expansion lattice light sheet image nanoscale spatial relationships between proteins across thickness mouse cortex entire Drosophila including synaptic at dendritic spines, myelination...
Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
Journal Article Large Area SEM Backscatter Imaging of GABA Post-Embedding Immunogold, Web-Based Crowdsourcing Segmentation and Local Image Processing for Rigorous Quantification Get access Cheryl Clarkson, Clarkson George Washington University, Nanofabrication Center, Washington, DC, USA Corresponding author: cclarkson12@gwu.edu Search other works by this author on: Oxford Academic Google Scholar Christopher T Zugates, Zugates arivis AG, Christine A Brantner, Brantner Matthias Rust, Rust...
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