A5059140736- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Invertebrate Immune Response Mechanisms
- Animal Behavior and Reproduction
- Physiological and biochemical adaptations
- Photoreceptor and optogenetics research
- Axon Guidance and Neuronal Signaling
- Neural dynamics and brain function
- Plant Molecular Biology Research
- Zebrafish Biomedical Research Applications
- Plant and animal studies
- Circadian rhythm and melatonin
- Neural Networks and Applications
- Insect Utilization and Effects
- Neuroscience and Neuropharmacology Research
- Bayesian Modeling and Causal Inference
- Viral Infectious Diseases and Gene Expression in Insects
- Data Management and Algorithms
- Developmental Biology and Gene Regulation
- Video Surveillance and Tracking Methods
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Face and Expression Recognition
- Mitochondrial Function and Pathology
- Memory and Neural Mechanisms
Janelia Research Campus
2016-2025
Howard Hughes Medical Institute
2016-2025
MRC Laboratory of Molecular Biology
2017-2025
University of Cologne
2024-2025
University of Cambridge
2003-2024
The University of Queensland
2024
University of California, Berkeley
2024
Columbia University
2009-2024
George Washington University Virginia Campus
2022
Helix (United States)
2013-2020
The identification of active neurons and circuits in vivo is a fundamental challenge understanding the neural basis behavior. Genetically encoded calcium (Ca(2+)) indicators (GECIs) enable quantitative monitoring cellular-resolution activity during However, such require online within limited field view. Alternatively, post hoc staining immediate early genes (IEGs) indicates highly cells entire brain, albeit with poor temporal resolution. We designed fluorescent sensor, CaMPARI, that combines...
A single nervous system can generate many distinct motor patterns. Identifying which neurons and circuits control behaviors has been a laborious piecemeal process, usually for one observer-defined behavior at time. We present fundamentally different approach to neuron-behavior mapping. optogenetically activated 1054 identified neuron lines in Drosophila larvae tracked the behavioral responses from 37,780 animals. Application of multiscale unsupervised structure learning methods data enabled...
Brains contain networks of interconnected neurons and so knowing the network architecture is essential for understanding brain function. We therefore mapped synaptic-resolution connectome an entire insect ( Drosophila larva) with rich behavior, including learning, value computation, action selection, comprising 3016 548,000 synapses. characterized neuron types, hubs, feedforward feedback pathways, as well cross-hemisphere brain-nerve cord interactions. found pervasive multisensory...
Significance A previously unidentified set of thermosensory neurons embedded in the olfactory organ Drosophila larva is shown to be required drive animal up temperature gradients toward preferred environments. Optogenetics and optical neurophysiology reveal efficient sensory encoding both favorable (warming) unfavorable (cooling) stimuli for distinct components thermotactic strategy by this one neurons. Cooling-evoked activation used curtail forward movements directions; warming-evoked...
Rapid and efficient escape behaviors in response to noxious sensory stimuli are essential for protection survival. Yet, how transformed coordinated remains poorly understood. In Drosophila larvae, trigger sequential body bending corkscrew-like rolling behavior. We identified a population of interneurons the nerve cord Drosophila, termed Down-and-Back (DnB) neurons, that activated by heat, promote nociceptive behavior, required robust responses stimuli. Electron microscopic circuit...
The brain adaptively integrates present sensory input, past experience, and options for future action. insect mushroom body exemplifies how a central structure brings about such integration. Here we use combination of systematic single-cell labeling, connectomics, transgenic silencing, activation experiments to study the at resolution, focusing on behavioral architecture its input output neurons (MBINs MBONs), intrinsic APL neuron. Our results reveal identity morphology almost all these 44...
All organisms react to noxious and mechanical stimuli but we still lack a complete understanding of cellular molecular mechanisms by which somatosensory information is transformed into appropriate motor outputs. The small number neurons excellent genetic tools make Drosophila larva an especially tractable model system in address this problem. We developed high throughput assays with can simultaneously expose more than 1,000 larvae per man-hour precisely timed heat, vibration, air current, or...
Abstract Molecular profiles of neurons influence neural development and function but bridging the gap between genes, circuits, behavior has been very difficult. Here we used single cell RNAseq to generate a complete gene expression atlas Drosophila larval central nervous system composed 131,077 cells across three developmental stages (1 h, 24 h 48 after hatching). We identify 67 distinct clusters based on patterns expression. These include 31 functional mature neuron clusters, 1 ring gland...
Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy...
Visual systems transduce, process and transmit light-dependent environmental cues. Computation of visual features depends on photoreceptor neuron types (PR) present, organization the eye wiring underlying neural circuit. Here, we describe circuit architecture system Drosophila larvae by mapping synaptic diagram neurotransmitters. By contacting different targets, two larval PR-subtypes create converging pathways potentially computation ambient light intensity temporal changes already within...
Sensory navigation results from coordinated transitions between distinct behavioral programs. During chemotaxis in the Drosophila melanogaster larva, detection of positive odor gradients extends runs while negative promote stops and turns. This algorithm represents a foundation for control sensory across phyla. In present work, we identified an olfactory descending neuron, PDM-DN, which plays pivotal role organization turns response to graded changes concentrations. Artificial activation...
Abstract Efforts to map neural circuits have been galvanized by the development of genetic technologies that permit manipulation targeted sets neurons in brains freely behaving animals. The success these efforts relies on experimenter’s ability target arbitrarily small subsets for manipulation, but such specificity targeting cannot routinely be achieved using existing methods. In Drosophila melanogaster, a widely-used technique refined cell type-specific is Split GAL4 system, which augments...
Nervous systems have the ability to select appropriate actions and action sequences in response sensory cues. The circuit mechanisms by which nervous achieve choice, stability transitions between behaviors are still incompletely understood. To identify neurons brain areas involved controlling these processes, we combined a large-scale neuronal inactivation screen with automated detection mechanosensory cue Drosophila larva. We analyzed from 2.9x105 larvae identified 66 candidate lines for...
Animal behavior is shaped both by evolution and individual experience. Parallel brain pathways encode innate learned valences of cues, but the way in which they are integrated during action-selection not well understood. We used electron microscopy to comprehensively map with synaptic resolution all neurons downstream mushroom body (MB) output (encoding valences) characterized their patterns interaction lateral horn (LH) Drosophila larva. The connectome revealed multiple convergence neuron...
During the development of neural circuitry, neurons different kinds establish specific synaptic connections by selecting appropriate targets from large numbers alternatives. The range alternative is reduced well organised patterns growth, termination, and branching that deliver terminals pre- postsynaptic partners to restricted volumes developing nervous system. We use axons embryonic Drosophila sensory as a model system in which study way growing are guided terminate mediolateral positions...
ABSTRACT Mapping brain function to structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva simple enough be tractable, yet complex interesting. It features about 10,000 neurons and capable of various taxes, kineses Pavlovian conditioning. All its are currently being mapped into light-microscopical atlas, Gal4 strains generated experimentally access one at time. In addition, electron microscopic reconstruction nervous system seems within reach. Notably,...
Rhythmic motor patterns underlying many types of locomotion are thought to be produced by central pattern generators (CPGs). Our knowledge how CPG networks generate in complex nervous systems remains incomplete, despite decades work a variety model organisms. Substrate borne Drosophila larvae is driven waves muscular contraction that propagate through multiple body segments. We use the circuitry crawling larval as try understand segmentally coordinated rhythmic generated. Whereas muscles,...