A5056589572- Photoreceptor and optogenetics research
- Computational Drug Discovery Methods
- Medical Imaging Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- stochastic dynamics and bifurcation
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Ion channel regulation and function
University of Chicago
2020
Howard Hughes Medical Institute
2013-2015
Janelia Research Campus
2015
Helix (United States)
2015
The identification of active neurons and circuits in vivo is a fundamental challenge understanding the neural basis behavior. Genetically encoded calcium (Ca(2+)) indicators (GECIs) enable quantitative monitoring cellular-resolution activity during However, such require online within limited field view. Alternatively, post hoc staining immediate early genes (IEGs) indicates highly cells entire brain, albeit with poor temporal resolution. We designed fluorescent sensor, CaMPARI, that combines...
Fluorescent protein-based sensors for detecting neuronal activity have been developed largely based on non-neuronal screening systems. However, the dynamics of state variables (e.g., voltage, calcium, etc.) are typically very rapid compared to those non-excitable cells. We an electrical stimulation and fluorescence imaging platform dissociated rat primary cultures. describe its use in testing genetically-encoded calcium indicators (GECIs). Efficient GECI expression was achieved using...