A5020129981- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Olfactory and Sensory Function Studies
- Mathematical Biology Tumor Growth
- Gene Regulatory Network Analysis
- Photoreceptor and optogenetics research
- Physiological and biochemical adaptations
- Microfluidic and Bio-sensing Technologies
- Evolution and Genetic Dynamics
- Molecular Communication and Nanonetworks
- Insect Pheromone Research and Control
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Mechanics and Biomechanics Studies
- Gear and Bearing Dynamics Analysis
- Adipose Tissue and Metabolism
- Insect Utilization and Effects
- Micro and Nano Robotics
- Neural dynamics and brain function
- Insect Pest Control Strategies
- Bacterial Genetics and Biotechnology
- Metallurgy and Material Forming
- Insect behavior and control techniques
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
Harvard University
2015-2021
Center for Pain and the Brain
2015-2021
Peking University
2009-2019
Center for Interdisciplinary Studies
2013
Center for Life Sciences
2009-2012
The sense of smell enables animals to react long-distance cues according learned and innate valences. Here, we have mapped with electron microscopy the complete wiring diagram Drosophila larval antennal lobe, an olfactory neuropil similar vertebrate bulb. We found a canonical circuit uniglomerular projection neurons (uPNs) relaying gain-controlled ORN activity mushroom body lateral horn. A second, parallel multiglomerular (mPNs) hierarchically connected local (LNs) selectively integrates...
Neural circuits for behavior transform sensory inputs into motor outputs in patterns with strategic value. Determining how neurons along a sensorimotor circuit contribute to this transformation is central understanding behavior. To do this, quantitative framework describe behavioral dynamics needed. In study, we built high-throughput optogenetic system Drosophila larva quantify the transformations underlying navigational We express CsChrimson, red-shifted variant of channelrhodopsin,...
Animal behavior is shaped both by evolution and individual experience. Parallel brain pathways encode innate learned valences of cues, but the way in which they are integrated during action-selection not well understood. We used electron microscopy to comprehensively map with synaptic resolution all neurons downstream mushroom body (MB) output (encoding valences) characterized their patterns interaction lateral horn (LH) Drosophila larva. The connectome revealed multiple convergence neuron...
We developed a multiple-channel microfluidic device for bacterial chemotaxis detection. Some characteristics such as easy operation, parallel sample adding design and fast result readout make this convenient most biology labs. The characteristic feature of the is agarose gel channels, which serve semi-permeable membrane. They can stop fluid flow prevent bacteria getting across, but permit diffusion small molecules. In fabrication process novel thermal-based method was used to control shape...
We develop a mean-field theory for Escherichia coli chemotaxis based on the coupled spatiotemporal dynamics of cell population and mean receptor methylation level field. This multiscale model connects cells' motility behavior with molecular pathway dynamics. It reveals simple scaling dependence velocity adaptation rate in exponential gradients. explains origin maximum velocity. Simulations our various stimuli profiles show quantitative agreements experiments. Moreover, it predicts surprising...
We study Escherichia coli chemotaxis behavior in environments with spatially and temporally varying attractant sources by developing a unique microfluidic system. Our measurements reveal frequency-dependent behavior. At low frequency, the E. population oscillates synchrony attractant. In contrast, fast-changing environments, response becomes smaller out of phase waveform. These observations are inconsistent well-known Keller-Segel equation. A new continuum model is proposed to describe level...
Significance Chemotaxis is a universal phenomenon whereby motile cells, like bacteria, navigate by following chemical gradients in their environment. Bacterial chemoreceptors can bind with specific chemoeffectors and transfer environmental signals to the cell. However, molecular mechanisms for chemoeffector binding signaling are not fully understood, rational design of bacteria respond new chemicals has been challenging. In this study, using combined experimental computational approach, we...
Significance Not all nutrient molecules act as attractant signals, and not attractants are good nutrients. Here, we study behaviors of bacterial cells in an environment with competing sources: One has a strong but poor nutrient, the other rich weak attractant. We find that, although initially attracted toward attractant, opposing gradient generated by consumption eventually becomes dominant when cell density reaches critical value. The then form band escaping “trap” migrating nutrient. Our...
A pathway-based mean-field theory (PBMFT) that incorporated the most recent quantitatively measured signaling pathway was recently proposed for E. coli chemotaxis in [G. Si, T. Wu, Q. Quyang, and Y. Tu, Phys. Rev. Lett., 109 (2012), 048101]. In this paper, we formally derive a new kinetic system of PBMFT under assumption methylation level is locally concentrated, whose turning operator takes into account dynamical intracellular hence more physically relevant. We recover by Si et al. as...
Abstract Animal behavior is shaped both by evolution and individual experience. In many species parallel brain pathways are thought to encode innate learnt drives as a result may link the same sensory cue different actions if in opposition. How these opposing integrated into single coherent action not well understood. insects, Mushroom Body Output Neurons (MBONs) Lateral Horn (LHNs) provide drives, respectively. However their patterns of convergence mechanisms which outputs used select We...
Abstract The sense of smell enables animals to react long-distance cues according learned and innate valences. Here, we have mapped with electron microscopy the complete wiring diagram Drosophila larval antennal lobe, an olfactory neuropil similar vertebrate bulb. We found a canonical circuit uniglomerular projection neurons (uPNs) relaying gain-controlled ORN activity mushroom body lateral horn. A second, parallel multiglomerular (mPNs) hierarchically connected local (LNs) selectively...
We study cell navigation in spatiotemporally complex environments by developing a microfluidic racetrack device that creates traveling wave with multiple peaks and tunable speed. find while the population-averaged chemotaxis drift speed increases for low speed, it decreases sharply high This reversed dependence of on is caused “barrier-crossing” phenomenon, where hops backwards from one peak attractant location to behind crossing an unfavorable (barrier) region concentrations. By using...
A pathway-based mean-field theory (PBMFT) was recently proposed for E. coli chemotaxis in [G. Si, T. Wu, Q. Quyang and Y. Tu, Phys. Rev. Lett., 109 (2012), 048101]. In this paper, we derived a new moment system of PBMFT by using the closure technique kinetic under assumption that methylation level is locally concentrated. The hyperbolic with linear convection terms. Under certain assumptions, can recover original model. Especially on difference made there be understood explicitly system. We...
Neural circuits for behavior transform sensory inputs into motor outputs in patterns with strategic value. Determining how neurons along a sensorimotor circuit contribute to this transformation is central understanding behavior. To do this, quantitative framework describe behavioral dynamics needed. Here, we built high-throughput optogenetic system Drosophila larva quantify the transformations underlying navigational We express CsChrimson, red-shifted variant of Channelrhodopsin, specific...
The Drosophila larva, a soft-body animal, can bend its body and roll efficiently to escape danger. However, contrary common belief, this rolling motion is not driven by the imbalance of gravity ground reaction forces. Through functional imaging ablation experiments, we demonstrate that sequential actuation axial muscles within an appropriate range angles critical for generating rolling. We model interplay between muscle contraction, hydrostatic skeleton deformation, body-environment...
Abstract Animals can identify an odorant type across a wide range of concentrations, as well detect changes in concentration for individual type. How olfactory representations are structured to support these functions remains poorly understood. Here, we studied how full complement ORNs the Drosophila larva encodes broad input space types and concentrations. We find that dose-response relationships odorants ORN follow Hill function with shared cooperativity but different activation...
ABSTRACT Olfactory systems employ combinatorial receptor codes for odors. Systematically generating stimuli that address the possibilities of an olfactory code poses unique challenges. Here, we present a stimulus method to probe code, demonstrated using Drosophila larva. This leverages set primary odorants, each which targets activity one neuron (ORN) type at optimal concentration. Our setup uses microfluidics mix any combination odorants on demand activate desired ORNs. We use this pattern...