A5087613872- Neurobiology and Insect Physiology Research
- Neuroscience and Neuropharmacology Research
- Olfactory and Sensory Function Studies
- Ion channel regulation and function
- Insect Pheromone Research and Control
- Receptor Mechanisms and Signaling
- Cell Image Analysis Techniques
- Spondyloarthritis Studies and Treatments
- Rheumatoid Arthritis Research and Therapies
- Neural dynamics and brain function
- Semantic Web and Ontologies
- Research Data Management Practices
- Library Science and Information Systems
- Systemic Lupus Erythematosus Research
- Osteoarthritis Treatment and Mechanisms
- Bone and Joint Diseases
- Advanced Fluorescence Microscopy Techniques
- Regulation of Appetite and Obesity
- Psoriasis: Treatment and Pathogenesis
- Antioxidant Activity and Oxidative Stress
- Infrared Thermography in Medicine
- Photoreceptor and optogenetics research
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
Gesellschaft zur Förderung angewandter Informatik
2024
Fraunhofer Society
2024
Fraunhofer Institute for Applied Information Technology
2021-2022
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2009-2021
University of Cologne
2009-2021
Satiety-signaling, pro-opiomelanocortin (POMC)-expressing neurons in the arcuate nucleus of hypothalamus play a pivotal role regulation energy homeostasis. Recent studies reported altered mitochondrial dynamics and decreased mitochondria- endoplasmic reticulum contacts POMC during diet-induced obesity. Since mitochondria crucial Ca2+ signaling, we investigated whether obesity alters handling these mice. In obesity, cellular properties including uptake capacity are impaired, an increased...
We used patch-clamp recordings and fast optical Ca(2+) imaging to characterize an acetylcholine-induced current (I(ACh)) in leg motoneurons of the stick insect Carausius morosus. Our long-term goal is better understand synaptic integrative properties sensory-motor system, which has served extremely successfully as a model study basic principles walking locomotion on network level. The experiments were performed under biophysically controlled conditions freshly dissociated avoid secondary...
Measuring variations of intracellular free calcium concentration through the changes in fluorescence a calcium-sensitive dye is ubiquitous technique neuroscience. Despite its popularity, confidence intervals (CIs) on estimated parameters dynamics models are seldom given. To address this issue, we have developed two-stage model for ratiometric measurements obtained with charge-coupled device (CCD) camera. Its first element embeds parametric into intensity and second probabilistically...
Fluorescence optical imaging technique (FOI) is a well-established and valid method for visualization of changes in micro vascularization at different organ systems. As increased an early feature joint inflammation, FOI promising to assess arthritis the hands. But usability limited assessors experience as measurement semi-quantitative using individual grading system such fluorescence activity score (FOIAS). The goal study was automatically thus, objectively analyze measured intensity...
Background: Psoriasis (Pso) is one of the most common chronic inflammatory skin diseases in Europe. Psoriatic arthritis (PsA) closely associated to Pso whereas manifestation appears usually years before PsA-related symptoms emerge. Up 30% patients develop PsA, biomarkers for its early detection are major importance. In changes synovial vascularisation appear first. Imaging might be useful musculoskeletal disease. Fluorescence-optical imaging (FOI) a new method detect microvascularisation...
Abstract Ca 2+ functions as an important intracellular signal for a wide range of cellular processes. These processes are selectively activated by controlled spatiotemporal dynamics the free cytosolic . Intracellular regulated numerous parameters. Here, we established new way to determine neuronal handling properties combining ‘added buffer’ approach (Neher and Augustine, 1992) with perforated patch-clamp recordings (Horn Marty, 1988). Since added buffer typically employs standard whole-cell...