A5083095386- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Developmental Biology and Gene Regulation
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Skin and Cellular Biology Research
- Invertebrate Immune Response Mechanisms
- Cancer-related gene regulation
- Retinal Development and Disorders
- Microtubule and mitosis dynamics
- Axon Guidance and Neuronal Signaling
- Protist diversity and phylogeny
- Kruppel-like factors research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Calcium signaling and nucleotide metabolism
- Plant Reproductive Biology
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- Cell Adhesion Molecules Research
- Barrier Structure and Function Studies
- Connexins and lens biology
- Erythrocyte Function and Pathophysiology
- Lysosomal Storage Disorders Research
University of Toronto
2013-2022
University of California, Los Angeles
1992-1996
University of Cologne
1987-1993
ABSTRACT We have studied the embryonic development of Drosophila hemocytes and their conversion into macrophages. Hemocytes derive exclusively from mesoderm head disperse along several invariant migratory paths throughout embryo. The origin is further supported by finding that in Bicaudal D, a mutation lacks all structures, twist snail double mutants, where no develops, do not form. All behave like homogenous population with respect to potential for phagocytosis. Thus, wild type, about...
Adhesion molecules of the cadherin superfamily have an important role during vertebrate development. The DE-cadherin homolog is first classic isolated from invertebrates. We report here that encoded by shotgun (shg) gene. shg expressed in most embryonic epithelia and decreases cells undergo epithelial-mesenchymal transitions like mesoderm or neural precursors. Removal both maternal zygotic function leads to severe defects all expressing shg, suggesting DE-cadherin, similar cadherins, has a...
Cadherins are involved in a variety of morphogenetic movements during animal development. However, it has been difficult to pinpoint the precise function cadherins processes due multifunctional nature cadherin requirement. The data presented here indicate that homophilic adhesion promoted by Drosophila E-cadherin (DE-cadherin) mediates two cell migration events oogenesis. In follicles, groups follicle cells, border cells and centripetal migrate on surface germline cells. We show as an...
ABSTRACT The complex embryonic phenotype of the six neurogenic mutations Notch, mastermind, big brain, Delta, Enhancer split and neuralized was analyzed by using different antibodies PlacZ markers, which allowed us to label most known tissues. Our results demonstrate that all mutants show abnormalities in many organs derived from three germ layers. Defects caused ectodermally tissues fell into two categories. First, cell types delaminate ectoderm (neuroblasts, sensory neurons, peripheral...
Epithelial tubes of the correct size and shape are vital for function lungs, kidneys, vascular system, yet little is known about epithelial tube regulation. Mutations in Drosophila gene sinuous have previously been shown to cause tracheal be elongated diameter increases. Our genetic analysis using a null mutation suggests that functions same pathway as septate junction genes neurexin scribble, but nervana 2, convoluted, varicose, cystic not shared by sinuous. molecular analyses reveal...
Cell rearrangements require dynamic changes in cell–cell contacts to maintain tissue integrity. We investigated the function of Cdc42 maintaining adherens junctions (AJs) and apical polarity Drosophila melanogaster neuroectodermal epithelium. About one third cells exit epithelium through ingression become neuroblasts. Cdc42-compromised embryos lost AJs neuroectoderm during neuroblast ingression. In contrast, when formation was suppressed, were maintained despite loss function. Loss caused an...
Laminins are heterotrimeric molecules found in all basement membranes. In mammals, they have been involved diverse developmental processes, from gastrulation to tissue maintenance. The Drosophila genome encodes two laminin alpha chains, one beta and Gamma, which form distinct trimers. So far, only mutations affecting or other trimer analysed. order study embryonic development the complete absence of laminins, we mutated gene encoding sole chain Drosophila, LanB1, so that no trimers can be...
α-catenin is a key mechanosensor that forms force-dependent interactions with F-actin, thereby coupling the cadherin-catenin complex to actin cytoskeleton at adherens junctions (AJs). However, molecular mechanisms by which engages F-actin under tension remained elusive. Here we show α1-helix of actin-binding domain (αcat-ABD) mechanosensing motif regulates tension-dependent binding and bundling. αcat-ABD containing an α1-helix-unfolding mutation (H1) shows enhanced in vitro. Although...
Analysis of the mechanisms that control epithelial polarization has revealed cues for are mediated by transmembrane proteins operate at apical, lateral, or basal surface cells. Whereas any given cell type only one two systems have been identified to date, we report here follicular epithelium in Drosophila ovaries uses three different mechanisms, each operating main domains. The arises through a mesenchymal–epithelial transition. Contact with basement membrane provides an initial cue leads...