A5023607846- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Axon Guidance and Neuronal Signaling
- Neurological disorders and treatments
- Parkinson's Disease Mechanisms and Treatments
- Nicotinic Acetylcholine Receptors Study
- Laser-Ablation Synthesis of Nanoparticles
- Neuropeptides and Animal Physiology
- Cellular transport and secretion
- Neurogenesis and neuroplasticity mechanisms
- Tuberous Sclerosis Complex Research
- Zebrafish Biomedical Research Applications
- Retinoids in leukemia and cellular processes
- Asthma and respiratory diseases
- Genetic Neurodegenerative Diseases
- Advanced Fluorescence Microscopy Techniques
- Electrochemical Analysis and Applications
- Attention Deficit Hyperactivity Disorder
- Wireless Power Transfer Systems
- Genetics and Neurodevelopmental Disorders
- Pain Mechanisms and Treatments
- Autism Spectrum Disorder Research
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
Université Laval
2004-2022
Montreal Clinical Research Institute
2020
Institut Universitaire en Santé Mentale de Québec
2016
Monitoring voltage dynamics in defined neurons deep the brain is critical for unraveling function of neuronal circuits but challenging due to limited performance existing tools. In particular, while genetically encoded indicators have shown promise optical detection transients, many exhibit low sensitivity when imaged under two-photon illumination. Previous studies thus fell short visualizing individual single trials. Here, we report ASAP2s, a novel indicator with improved sensitivity. By...
Abstract Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability perform subcellular light stimulation investigate localized been limited. Here, we introduce an all optical method for and monitoring Ca 2+ neurons that takes advantage plasmonic excitation gold nanoparticles (AuNPs). We show with confocal microscopy 800 nm laser pulse application onto neuron decorated few AuNPs triggers...
Anterolateral system neurons relay pain, itch, and temperature information from the spinal cord to pain-related brain regions, but differentiation of these their specific contribution pain perception remain poorly defined. Here, we show that most mouse embryonically express autonomic-system-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive targets, including parabrachial nucleus thalamus. We define Phox2a anterolateral neuron birth order, migration,...
Rationale: Urokinase plasminogen activator (uPA) interacts with its receptor on inflammatory and migrating cells to regulate extracellular matrix degradation, cell adhesion, activation. It is necessary for the development of an appropriate immune response involved in tissue remodeling. The PLAU gene codes this enzyme, located 10q24. This region has demonstrated evidence linkage a genome scan asthma sample from northeastern Quebec. Here, we hypothesized that uPA may function as regulator...
Neurodegenerative disorders refer to a group of diseases commonly associated with abnormal protein accumulation and aggregation in the central nervous system. However, exact role pathophysiology these remains unclear. This gap knowledge is due lack experimental models that allow for spatiotemporal control aggregation, investigation early dynamic events inclusion formation. Here, we report on development light-inducible (LIPA) system enables α-synuclein (α-syn) into insoluble deposits called...
The neurodevelopmental origin of hyperactivity disorder has been suggested to involve the dopaminergic system, but underlying mechanisms are still unknown. Here, transcription factors Lmx1a and Lmx1b shown be essential for midbrain (mDA) neuron excitatory synaptic inputs dendritic development. Strikingly, conditional knockout (cKO) Lmx1a/b in postmitotic mDA neurons results marked hyperactivity. In seeking target genes, we identify positively regulated Slitrk2 negatively Slitrk5. These two...
Stratum oriens-lacunosum moleculare interneurons (O-LM INs) represent the major element of hippocampal feedback inhibitory circuit, which provides inhibition to distal dendritic sites CA1 pyramidal neurons. Although intrinsic conductance profile and properties glutamatergic transmission O-LM INs have become a subject intense investigation, far less is known about synapses formed onto these cells. Here, we used whole-cell patch-clamp recordings in acute mouse slices study plasticity GABAergic...
Mesodiencephalic dopamine neurons play central roles in the regulation of a wide range brain functions, including voluntary movement and behavioral processes. These functions are served by distinct subtypes mesodiencephalic located substantia nigra pars compacta ventral tegmental area, which form nigrostriatal, mesolimbic, mesocortical pathways. Until now, mechanisms involved dopaminergic circuit formation remained largely unknown. Here, we show that Lmx1a, Lmx1b, Otx2 transcription factors...
Cell-surface molecules are dynamically regulated at the synapse to assemble and disassemble adhesive contacts that important for synaptogenesis tuning synaptic transmission. Metalloproteinases regulate cellular behaviors through processing of cell surface molecules. In present study, we evaluated role membrane-type metalloproteinases (MT-MMPs) in excitatory synaptogenesis. We find MT3-MMP MT5-MMP broadly expressed mouse cerebral cortex loss-of-function interferes with development dissociated...
Insulin signaling through the insulin receptor has long been studied in classic target organs, such as adipose tissue and skeletal muscle, where one of its effects is to increase glucose uptake. are present many areas brain, but functions brain outside feeding circuits not well defined. It proposed that hippocampal important for memory, deficient Alzheimer's disease, intranasal treatment improves cognition, mechanisms remain unclear do seem involve increased uptake by neurons. The molecular...
α-synuclein (α-syn) aggregation into insoluble deposits, referred to as Lewy bodies (LBs) is the paramount pathological hallmark of Parkinson's disease and related α-synucleinopathies. However, how these aggregates affect neuronal homeostasis leading neurodegeneration remains elusive. This gap in knowledge mainly due lack proper cellular animal models undertake such investigations. We have addressed this by developing a light-inducible protein (LIPA) system that allows for real-time...
α-Synuclein (α-syn) aggregation into insoluble deposits, referred to as Lewy bodies (LBs), is the paramount pathological hallmark of Parkinson's disease (PD) and related α-synucleinopathies. However, mechanism by which these aggregates contribute neurodegeneration remains unclear. This gap in knowledge due lack experimental models that allow for spatiotemporal control α-syn investigation early dynamic events associated with inclusion formation. Here, we report on development a...
Gold nanoparticles (AuNPs) have found numerous applications in nanomedicine view of their robustness, ease functionalization and low toxicity. Upon irradiation AuNPs by a pulsed ultrafast laser, various highly localized phenomena can be obtained including temperature rise, pressure wave, charge injection production nanobubbles close to the cellular membrane [1]. These used manipulate, optoperforate, transfect stimulate targeted cells [2-5]. Irradiating at 800 nm optically biological...
Summary Anterolateral system neurons relay pain, itch and temperature information from the spinal cord to pain-related brain regions, but differentiation of these their specific contribution pain perception remain poorly defined. Here, we show that virtually all mouse embryonically express autonomic system-associated Paired-like homeobox 2A (Phox2a) transcription factor innervate nociceptive targets, including parabrachial nucleus thalamus. We define Phox2a anterolateral neuron birth order,...