A5077533094- Alzheimer's disease research and treatments
- Protein Structure and Dynamics
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Nerve injury and regeneration
- Computational Drug Discovery Methods
- Advanced Fluorescence Microscopy Techniques
- Genomics and Phylogenetic Studies
- PARP inhibition in cancer therapy
- Neurological disorders and treatments
- Wnt/β-catenin signaling in development and cancer
- Machine Learning in Bioinformatics
- Parkinson's Disease Mechanisms and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Neurological Disease Mechanisms and Treatments
- Heat shock proteins research
- Pleistocene-Era Hominins and Archaeology
- Drug Transport and Resistance Mechanisms
- Axon Guidance and Neuronal Signaling
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Neuroinflammation and Neurodegeneration Mechanisms
Universidade Federal do Rio de Janeiro
2006-2021
Institute of Biochemistry
2014
Max Planck Society
2010
Max Planck Institute for Evolutionary Anthropology
2010
University of Göttingen
2008-2009
European Synchrotron Radiation Facility
2005
Alzheimer's disease (AD) is a devastating neurodegenerative disorder and major medical problem. Here, we have investigated the impact of amyloid-β (Aβ) oligomers, AD-related neurotoxins, in brains rats adult nonhuman primates (cynomolgus macaques). Soluble Aβ oligomers are known to accumulate AD patients correlate with disease-associated cognitive dysfunction. When injected into lateral ventricle macaques, diffused brain accumulated several regions associated memory functions. Cardinal...
Abstract Alzheimer's disease (AD) is a devastating neurological disorder that still lacks an effective treatment, and this has stimulated intense pursuit of disease‐modifying therapeutics. Given the increasingly recognized link between AD defective brain insulin signaling, we investigated actions liraglutide, glucagon‐like peptide‐1 (GLP‐1) analog marketed for treatment type 2 diabetes, in experimental models AD. Insulin receptor pathology important feature brains impairs neuroprotective...
The flotillins/reggie proteins are associated with noncaveolar membrane microdomains and have been implicated in the regulation of a clathrin- caveolin-independent endocytosis pathway. Endocytosis is required for amyloidogenic processing amyloid precursor protein (APP) thus to initiate release neurotoxic β-amyloid peptide (Aβ), major component extracellular plaques found brains Alzheimer's disease patients. Here, we report that small interference RNA-mediated downregulation flotillin-2...
The amyloid precursor protein (APP) is implied both in cell growth and differentiation neurodegenerative processes Alzheimer disease. Regulated proteolysis of APP generates biologically active fragments such as the neuroprotective secreted ectodomain sAPPalpha neurotoxic beta-amyloid peptide. Furthermore, it has been suggested that intact transmembrane plays a signaling role, which might be important for normal synaptic plasticity neuronal dysfunction dementia. To understand signaling, we...
Intracellular pH is an important indicator for cellular metabolism and pathogenesis. sensing in living cells has been achieved using a number of synthetic organic dyes genetically expressible sensor proteins, even allowing the specific targeting intracellular organelles. Ideally, class encodeable sensors need to cover relevant ranges. We present FRET-based platform, based on modulation YFP acceptor fluorophores fusion construct with ECFP. The concurrent loss overlap integral upon...
The β-amyloid peptide (Aβ), the major component of senile plaques found in brains Alzheimer's disease patients, is derived from proteolytic processing a transmembrane glycoprotein known as amyloid precursor protein (APP). Human APP exists various isoforms, which ones contain 695, 751, and 770 amino acids. Proteolytic cleavage by α- or β-secretases releases extracellular soluble fragments sAPPα sAPPβ, respectively. Despite fact that plays important roles both physiological pathological...
The amyloid precursor protein (APP) is well known for giving rise to the amyloid-β peptide and its role in Alzheimer's disease. Much less known, however, on physiological roles of APP development plasticity central nervous system. We have used phage display a library identify high-affinity ligands purified recombinant human sAPPα695 (the soluble, secreted ectodomain from main neuronal isoform). Two peptides thus selected exhibited significant homologies with conserved extracellular domain...
Insulin signaling through the insulin receptor has long been studied in classic target organs, such as adipose tissue and skeletal muscle, where one of its effects is to increase glucose uptake. are present many areas brain, but functions brain outside feeding circuits not well defined. It proposed that hippocampal important for memory, deficient Alzheimer's disease, intranasal treatment improves cognition, mechanisms remain unclear do seem involve increased uptake by neurons. The molecular...
Brain-expressed proteins that have undergone functional change during human evolution may contribute to cognitive capacities, and also leave us vulnerable specifically diseases, such as schizophrenia, autism or Alzheimer’s disease. In order search systematically for those changed the most might brain function pathology, all with orthologs in chimpanzee, orangutan rhesus macaque annotated being expressed on surface of cells central nervous system were ordered by number human-specific amino...
Alzheimer's disease (AD) is a debilitating neurodegenerative disorder and major health problem worldwide. The lack of effective drugs to treat AD stimulates an intense pursuit disease-modifying therapeutics. However, impediment progress may lie in fundamental differences between humans animal model species, largely rodents. Disruption hippocampal insulin signaling has recently been described the brains patients models disease, which could contribute cognitive impairment this disease....
DJ-1 (PARK7) is the encoded protein from one of four chromosomal loci associated with autosomal recessive, early onset parkinsonism. While evidence genetic and biochemical studies in murine human cell lines indicates a role for as an antioxidant forming homodimer, function still remains controversial. In our study, we demonstrate interaction by co-immunoprecipitation between BAG1 (Bcl-2-associated athanogene-1), which was found to be regulator Hsp70/Hsc70 family molecular chaperones inducing...
For small-angle X-ray scattering (SAXS) experiments requiring high angular resolution and dynamic range, the Bonse-Hart camera [1] is still an attractive technique.The low divergence flux of undulator beam permits to use a crossed-analyser configuration, obtain unsmeared data on absolute scale (without calibration standard) within acquisition time few minutes.However, double-crystal diffractometers with multibounce channel-cut crystals show rocking curves which depart considerably in their...