A5020048204- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
- Alzheimer's disease research and treatments
- Fractal and DNA sequence analysis
- Nanopore and Nanochannel Transport Studies
- RNA Interference and Gene Delivery
- Lysosomal Storage Disorders Research
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- SARS-CoV-2 and COVID-19 Research
- Attention Deficit Hyperactivity Disorder
- Machine Learning in Bioinformatics
- Amino Acid Enzymes and Metabolism
- Tryptophan and brain disorders
- Histone Deacetylase Inhibitors Research
- Respiratory viral infections research
- Carbohydrate Chemistry and Synthesis
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Genetic Neurodegenerative Diseases
- Electrostatics and Colloid Interactions
- Retinal Development and Disorders
- Infectious Encephalopathies and Encephalitis
- Microtubule and mitosis dynamics
McGill University
2019-2022
Montreal Neurological Institute and Hospital
2019-2022
Simon Fraser University
2018-2021
National Institute of Neurological Disorders and Stroke
2020
National Institutes of Health
2020
Icahn School of Medicine at Mount Sinai
2020
keiyu Hospital
2020
Keio University
2020
University of Saskatchewan
2012-2014
Saskatoon Medical Imaging
2012
Abstract Background SMPD1 (acid‐sphingomyelinase) variants have been associated with Parkinson's disease in recent studies. The objective of this study was to further investigate the role mutations PD. Methods sequenced 3 cohorts (Israel Ashkenazi Jewish cohort, Montreal/Montpellier, and New York), including 1592 PD patients 975 controls. Additional data were available for 10,709 Acid‐sphingomyelinase activity measured by a mass spectrometry‐based assay York cohort. α‐Synuclein levels vitro...
α-Synuclein is an intrinsically disordered protein of 140 amino acids which abundant in dopaminergic neurons. Misfolding and aggregation α-synuclein leads to the formation Lewy bodies inside neurons hallmark Parkinson's disease related dementias. Here we show by nanopore analysis that recreational drug, methamphetamine, binds N-terminus causes a conformational change cannot be detected circular dichroism spectroscopy. The results suggest mechanism for psychoactivity methamphetamine as well...
α-Synuclein (AS) is an intrinsically disordered protein that can misfold and aggregate to form Lewy bodies in dopaminergic neurons, a classic hallmark of Parkinson's disease. The binding Cu(II) dopamine AS was evaluated by nanopore analysis with α-hemolysin. In the absence Cu(II), wild-type (1 μM) readily translocated through pore blockade current of--85 pA, but mostly bumping events were observed presence 25 μM Cu(II). A site N-terminus confirmed, because had no effect on event profile...
The pathological hallmark of Parkinson's disease (PD) is Lewy bodies that form within the brain from aggregated forms α-synuclein (α-syn). These toxic α-syn aggregates are transferred cell to by release fibrils dying neurons into extracellular environment, followed their subsequent uptake neighboring cells. This process leads spreading pathology throughout in a prion-like manner. Identifying new pathways hinder internalization such high interest for downstream potential exploitation as way...
Neurodegenerative disorders refer to a group of diseases commonly associated with abnormal protein accumulation and aggregation in the central nervous system. However, exact role pathophysiology these remains unclear. This gap knowledge is due lack experimental models that allow for spatiotemporal control aggregation, investigation early dynamic events inclusion formation. Here, we report on development light-inducible (LIPA) system enables α-synuclein (α-syn) into insoluble deposits called...
Rasagiline (N-propargyl-1-R-aminoindan) and selegiline (1-deprenyl) are MAO-B inhibitors which used in the treatment of Parkinson's disease. The binding rasagiline, selegiline, their metabolites including 1-aminoindan, 2-aminoindan, methamphetamine to α-synuclein was investigated by nanopore analysis isothermal titration calorimetry. Blockade current histograms alone give a peak at −86 pA is due translocation protein through pore. In presence rasagiline R-1-aminoindan, this shifts about −80...
Recent findings showed that preformed fibrils (PFFs) of misfolded proteins, including α-synuclein (α-syn) and amyloid-β (Aβ), activate EGFR in cell cultures animal models amyloid propagation. Comparing these supramolecular structures to normal ligands such as EGF HB-EGF suggests PFFs might trigger the formation high order clustering stimulates aggregation tyrosine kinase domain (EGFR-TKD) which is known form fibrils. Subsequently, self-assembled fibril EGFR-TKDs itself can serve a seed...
Chaos Game Representation (CGR) is an iterative mapping method which can convert a nucleotide sequence into unique and scale-independent image. In CGR image of DNA both global local patterns are displayed. These images allow us to investigate hidden self-similar in genomic sequences. this paper the fractal properties these have been quantified by dimension calculation quantitative characteristics considered multifractal analysis evaluate possible evolutionary mitochondrial (mtDNA) 10...
Chaos game representation (CGR) has been proposed as a method to convert genomic sequences scale-independent and unique images. Many useful properties applications have indicated for these In this paper, new procedure three- dimensional visualization of is introduced based on Game Representation algorithm. instead four points bases in nucleotide sequences, eight are considered corners cube. Four different nucleotides protein coding regions the sequence (A, C, G T) non- The mapping equations...
Chaos Game Representation (CGR) is an iterative mapping method which can convert a nucleotide sequence to unique and scale-independent image.There are some studies indicating different applications of CGR images sequences but there still limited information on their possible application in phylogenetics.In this study we have tried evolutionary evaluation mitochondrial DNA (mtDNA) species using image processing tools.In the algorithm chaos game for four points as bases were coded Matlab 7.0...
α-synuclein (α-syn) aggregation into insoluble deposits, referred to as Lewy bodies (LBs) is the paramount pathological hallmark of Parkinson's disease and related α-synucleinopathies. However, how these aggregates affect neuronal homeostasis leading neurodegeneration remains elusive. This gap in knowledge mainly due lack proper cellular animal models undertake such investigations. We have addressed this by developing a light-inducible protein (LIPA) system that allows for real-time...
The delivery of hydrophobic therapeutic agents to tumors is a challenge in the treatment cancers. Here, we review recent advances coiled-coil protein origami and discuss proposed programmable structure, switchable by kinase A/phosphatase switch, as an example functionalization for designing future nanorobots.
α-Synuclein (α-syn) aggregation into insoluble deposits, referred to as Lewy bodies (LBs), is the paramount pathological hallmark of Parkinson's disease (PD) and related α-synucleinopathies. However, mechanism by which these aggregates contribute neurodegeneration remains unclear. This gap in knowledge due lack experimental models that allow for spatiotemporal control α-syn investigation early dynamic events associated with inclusion formation. Here, we report on development a...